Amino acid dipepetide frequency for Bacillus sp. (strain OxB-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.342AlaAla: 7.342 ± 0.126	0.639AlaCys: 0.639 ± 0.025	4.366AlaAsp: 4.366 ± 0.071	5.962AlaGlu: 5.962 ± 0.094	3.575AlaPhe: 3.575 ± 0.061	6.332AlaGly: 6.332 ± 0.08	1.408AlaHis: 1.408 ± 0.04	6.343AlaIle: 6.343 ± 0.081	4.777AlaLys: 4.777 ± 0.065	7.937AlaLeu: 7.937 ± 0.1	2.363AlaMet: 2.363 ± 0.045	2.708AlaAsn: 2.708 ± 0.057	2.491AlaPro: 2.491 ± 0.053	2.491AlaGln: 2.491 ± 0.051	3.276AlaArg: 3.276 ± 0.059	4.401AlaSer: 4.401 ± 0.071	4.07AlaThr: 4.07 ± 0.066	6.272AlaVal: 6.272 ± 0.088	0.635AlaTrp: 0.635 ± 0.027	2.489AlaTyr: 2.489 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.428CysAla: 0.428 ± 0.022	0.079CysCys: 0.079 ± 0.009	0.35CysAsp: 0.35 ± 0.019	0.405CysGlu: 0.405 ± 0.023	0.238CysPhe: 0.238 ± 0.017	0.642CysGly: 0.642 ± 0.033	0.2CysHis: 0.2 ± 0.014	0.437CysIle: 0.437 ± 0.023	0.325CysLys: 0.325 ± 0.02	0.567CysLeu: 0.567 ± 0.026	0.148CysMet: 0.148 ± 0.011	0.217CysAsn: 0.217 ± 0.015	0.369CysPro: 0.369 ± 0.021	0.208CysGln: 0.208 ± 0.016	0.299CysArg: 0.299 ± 0.015	0.421CysSer: 0.421 ± 0.024	0.416CysThr: 0.416 ± 0.02	0.35CysVal: 0.35 ± 0.021	0.066CysTrp: 0.066 ± 0.009	0.199CysTyr: 0.199 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.12AspAla: 4.12 ± 0.073	0.344AspCys: 0.344 ± 0.019	2.639AspAsp: 2.639 ± 0.059	4.783AspGlu: 4.783 ± 0.072	2.42AspPhe: 2.42 ± 0.047	4.009AspGly: 4.009 ± 0.077	1.173AspHis: 1.173 ± 0.031	3.886AspIle: 3.886 ± 0.07	2.573AspLys: 2.573 ± 0.052	5.131AspLeu: 5.131 ± 0.082	1.475AspMet: 1.475 ± 0.041	1.593AspAsn: 1.593 ± 0.04	2.304AspPro: 2.304 ± 0.054	1.785AspGln: 1.785 ± 0.041	2.844AspArg: 2.844 ± 0.058	2.742AspSer: 2.742 ± 0.052	2.414AspThr: 2.414 ± 0.049	4.286AspVal: 4.286 ± 0.075	0.661AspTrp: 0.661 ± 0.025	1.993AspTyr: 1.993 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.422GluAla: 6.422 ± 0.081	0.335GluCys: 0.335 ± 0.021	4.042GluAsp: 4.042 ± 0.064	7.756GluGlu: 7.756 ± 0.115	2.545GluPhe: 2.545 ± 0.051	4.889GluGly: 4.889 ± 0.083	1.505GluHis: 1.505 ± 0.042	5.241GluIle: 5.241 ± 0.08	5.869GluLys: 5.869 ± 0.091	7.475GluLeu: 7.475 ± 0.099	2.754GluMet: 2.754 ± 0.054	3.07GluAsn: 3.07 ± 0.061	2.321GluPro: 2.321 ± 0.048	3.61GluGln: 3.61 ± 0.066	4.254GluArg: 4.254 ± 0.078	3.664GluSer: 3.664 ± 0.063	4.389GluThr: 4.389 ± 0.074	5.333GluVal: 5.333 ± 0.085	0.865GluTrp: 0.865 ± 0.03	1.982GluTyr: 1.982 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.26PheAla: 3.26 ± 0.056	0.296PheCys: 0.296 ± 0.018	2.482PheAsp: 2.482 ± 0.05	2.905PheGlu: 2.905 ± 0.054	2.211PhePhe: 2.211 ± 0.052	3.539PheGly: 3.539 ± 0.063	1.04PheHis: 1.04 ± 0.037	3.554PheIle: 3.554 ± 0.074	1.945PheLys: 1.945 ± 0.042	4.426PheLeu: 4.426 ± 0.096	1.189PheMet: 1.189 ± 0.04	1.612PheAsn: 1.612 ± 0.037	1.743PhePro: 1.743 ± 0.038	1.5PheGln: 1.5 ± 0.033	1.839PheArg: 1.839 ± 0.043	2.897PheSer: 2.897 ± 0.059	2.644PheThr: 2.644 ± 0.054	3.004PheVal: 3.004 ± 0.057	0.458PheTrp: 0.458 ± 0.021	1.472PheTyr: 1.472 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	5.46GlyAla: 5.46 ± 0.078	0.599GlyCys: 0.599 ± 0.027	3.544GlyAsp: 3.544 ± 0.066	4.994GlyGlu: 4.994 ± 0.073	3.297GlyPhe: 3.297 ± 0.055	5.14GlyGly: 5.14 ± 0.095	1.526GlyHis: 1.526 ± 0.041	5.74GlyIle: 5.74 ± 0.079	5.199GlyLys: 5.199 ± 0.081	6.91GlyLeu: 6.91 ± 0.099	2.351GlyMet: 2.351 ± 0.05	2.707GlyAsn: 2.707 ± 0.062	1.954GlyPro: 1.954 ± 0.043	2.544GlyGln: 2.544 ± 0.057	3.3GlyArg: 3.3 ± 0.063	4.138GlySer: 4.138 ± 0.063	4.354GlyThr: 4.354 ± 0.077	5.344GlyVal: 5.344 ± 0.07	0.834GlyTrp: 0.834 ± 0.034	2.652GlyTyr: 2.652 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.529HisAla: 1.529 ± 0.039	0.189HisCys: 0.189 ± 0.012	1.047HisAsp: 1.047 ± 0.031	1.41HisGlu: 1.41 ± 0.035	1.081HisPhe: 1.081 ± 0.033	1.505HisGly: 1.505 ± 0.04	0.615HisHis: 0.615 ± 0.026	1.434HisIle: 1.434 ± 0.037	0.846HisLys: 0.846 ± 0.027	2.105HisLeu: 2.105 ± 0.048	0.55HisMet: 0.55 ± 0.024	0.636HisAsn: 0.636 ± 0.024	1.256HisPro: 1.256 ± 0.04	0.761HisGln: 0.761 ± 0.028	1.024HisArg: 1.024 ± 0.034	1.168HisSer: 1.168 ± 0.035	1.013HisThr: 1.013 ± 0.032	1.53HisVal: 1.53 ± 0.038	0.216HisTrp: 0.216 ± 0.016	0.799HisTyr: 0.799 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.448IleAla: 6.448 ± 0.088	0.499IleCys: 0.499 ± 0.021	4.211IleAsp: 4.211 ± 0.07	5.511IleGlu: 5.511 ± 0.085	2.821IlePhe: 2.821 ± 0.059	6.206IleGly: 6.206 ± 0.08	1.68IleHis: 1.68 ± 0.038	5.062IleIle: 5.062 ± 0.084	2.966IleLys: 2.966 ± 0.063	6.581IleLeu: 6.581 ± 0.1	1.678IleMet: 1.678 ± 0.041	2.304IleAsn: 2.304 ± 0.047	3.254IlePro: 3.254 ± 0.061	2.85IleGln: 2.85 ± 0.053	3.8IleArg: 3.8 ± 0.077	4.441IleSer: 4.441 ± 0.08	3.955IleThr: 3.955 ± 0.071	5.646IleVal: 5.646 ± 0.08	0.616IleTrp: 0.616 ± 0.026	2.03IleTyr: 2.03 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.452LysAla: 4.452 ± 0.07	0.271LysCys: 0.271 ± 0.017	3.148LysAsp: 3.148 ± 0.059	5.875LysGlu: 5.875 ± 0.082	1.79LysPhe: 1.79 ± 0.045	4.023LysGly: 4.023 ± 0.064	1.019LysHis: 1.019 ± 0.035	3.638LysIle: 3.638 ± 0.064	4.54LysLys: 4.54 ± 0.087	5.143LysLeu: 5.143 ± 0.072	2.167LysMet: 2.167 ± 0.049	2.367LysAsn: 2.367 ± 0.051	2.225LysPro: 2.225 ± 0.055	2.441LysGln: 2.441 ± 0.045	3.331LysArg: 3.331 ± 0.062	3.197LysSer: 3.197 ± 0.064	3.467LysThr: 3.467 ± 0.062	4.091LysVal: 4.091 ± 0.067	0.748LysTrp: 0.748 ± 0.029	1.719LysTyr: 1.719 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	8.357LeuAla: 8.357 ± 0.126	0.541LeuCys: 0.541 ± 0.025	5.153LeuAsp: 5.153 ± 0.081	6.768LeuGlu: 6.768 ± 0.098	4.942LeuPhe: 4.942 ± 0.1	6.461LeuGly: 6.461 ± 0.1	2.131LeuHis: 2.131 ± 0.05	6.802LeuIle: 6.802 ± 0.103	5.533LeuLys: 5.533 ± 0.078	10.58LeuLeu: 10.58 ± 0.157	2.421LeuMet: 2.421 ± 0.05	3.504LeuAsn: 3.504 ± 0.057	4.245LeuPro: 4.245 ± 0.061	3.81LeuGln: 3.81 ± 0.07	4.243LeuArg: 4.243 ± 0.069	6.331LeuSer: 6.331 ± 0.071	5.555LeuThr: 5.555 ± 0.08	6.376LeuVal: 6.376 ± 0.078	0.73LeuTrp: 0.73 ± 0.031	2.999LeuTyr: 2.999 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.475MetAla: 2.475 ± 0.056	0.117MetCys: 0.117 ± 0.011	1.689MetAsp: 1.689 ± 0.041	2.525MetGlu: 2.525 ± 0.052	0.969MetPhe: 0.969 ± 0.033	1.834MetGly: 1.834 ± 0.045	0.426MetHis: 0.426 ± 0.02	1.948MetIle: 1.948 ± 0.052	2.606MetLys: 2.606 ± 0.055	2.589MetLeu: 2.589 ± 0.042	0.903MetMet: 0.903 ± 0.031	1.416MetAsn: 1.416 ± 0.031	1.152MetPro: 1.152 ± 0.033	0.993MetGln: 0.993 ± 0.03	1.348MetArg: 1.348 ± 0.036	1.654MetSer: 1.654 ± 0.038	1.984MetThr: 1.984 ± 0.046	1.764MetVal: 1.764 ± 0.047	0.183MetTrp: 0.183 ± 0.014	0.722MetTyr: 0.722 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.756AsnAla: 2.756 ± 0.059	0.275AsnCys: 0.275 ± 0.018	1.815AsnAsp: 1.815 ± 0.042	3.163AsnGlu: 3.163 ± 0.074	1.427AsnPhe: 1.427 ± 0.037	3.072AsnGly: 3.072 ± 0.061	0.762AsnHis: 0.762 ± 0.029	2.562AsnIle: 2.562 ± 0.053	1.934AsnLys: 1.934 ± 0.056	3.24AsnLeu: 3.24 ± 0.062	1.092AsnMet: 1.092 ± 0.03	1.28AsnAsn: 1.28 ± 0.047	2.02AsnPro: 2.02 ± 0.046	1.364AsnGln: 1.364 ± 0.041	2.188AsnArg: 2.188 ± 0.05	1.868AsnSer: 1.868 ± 0.051	1.784AsnThr: 1.784 ± 0.041	2.594AsnVal: 2.594 ± 0.053	0.433AsnTrp: 0.433 ± 0.022	1.202AsnTyr: 1.202 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	2.979ProAla: 2.979 ± 0.066	0.213ProCys: 0.213 ± 0.014	2.426ProAsp: 2.426 ± 0.05	3.275ProGlu: 3.275 ± 0.063	2.082ProPhe: 2.082 ± 0.049	2.634ProGly: 2.634 ± 0.063	0.841ProHis: 0.841 ± 0.033	2.868ProIle: 2.868 ± 0.052	2.102ProLys: 2.102 ± 0.05	3.721ProLeu: 3.721 ± 0.062	0.981ProMet: 0.981 ± 0.029	1.435ProAsn: 1.435 ± 0.043	1.225ProPro: 1.225 ± 0.041	1.137ProGln: 1.137 ± 0.04	1.255ProArg: 1.255 ± 0.037	2.471ProSer: 2.471 ± 0.049	1.976ProThr: 1.976 ± 0.047	3.34ProVal: 3.34 ± 0.065	0.342ProTrp: 0.342 ± 0.021	1.413ProTyr: 1.413 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.121GlnAla: 3.121 ± 0.057	0.187GlnCys: 0.187 ± 0.013	1.686GlnAsp: 1.686 ± 0.044	2.828GlnGlu: 2.828 ± 0.052	1.63GlnPhe: 1.63 ± 0.043	2.097GlnGly: 2.097 ± 0.053	0.781GlnHis: 0.781 ± 0.029	2.315GlnIle: 2.315 ± 0.05	2.29GlnLys: 2.29 ± 0.052	4.041GlnLeu: 4.041 ± 0.066	1.192GlnMet: 1.192 ± 0.036	1.375GlnAsn: 1.375 ± 0.037	1.279GlnPro: 1.279 ± 0.038	1.788GlnGln: 1.788 ± 0.042	1.664GlnArg: 1.664 ± 0.043	2.116GlnSer: 2.116 ± 0.046	1.967GlnThr: 1.967 ± 0.044	2.424GlnVal: 2.424 ± 0.049	0.401GlnTrp: 0.401 ± 0.019	1.141GlnTyr: 1.141 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	3.07ArgAla: 3.07 ± 0.059	0.246ArgCys: 0.246 ± 0.015	2.367ArgAsp: 2.367 ± 0.056	3.71ArgGlu: 3.71 ± 0.066	2.25ArgPhe: 2.25 ± 0.047	2.724ArgGly: 2.724 ± 0.062	1.009ArgHis: 1.009 ± 0.028	3.431ArgIle: 3.431 ± 0.065	3.484ArgLys: 3.484 ± 0.072	4.864ArgLeu: 4.864 ± 0.077	1.62ArgMet: 1.62 ± 0.044	2.062ArgAsn: 2.062 ± 0.053	1.672ArgPro: 1.672 ± 0.04	1.941ArgGln: 1.941 ± 0.046	2.297ArgArg: 2.297 ± 0.056	2.633ArgSer: 2.633 ± 0.052	2.59ArgThr: 2.59 ± 0.051	3.022ArgVal: 3.022 ± 0.058	0.455ArgTrp: 0.455 ± 0.022	1.595ArgTyr: 1.595 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	4.292SerAla: 4.292 ± 0.072	0.35SerCys: 0.35 ± 0.017	2.922SerAsp: 2.922 ± 0.054	3.814SerGlu: 3.814 ± 0.059	3.054SerPhe: 3.054 ± 0.062	4.681SerGly: 4.681 ± 0.077	1.146SerHis: 1.146 ± 0.035	4.555SerIle: 4.555 ± 0.072	3.115SerLys: 3.115 ± 0.055	5.746SerLeu: 5.746 ± 0.073	1.74SerMet: 1.74 ± 0.043	2.047SerAsn: 2.047 ± 0.062	2.258SerPro: 2.258 ± 0.048	1.755SerGln: 1.755 ± 0.041	2.651SerArg: 2.651 ± 0.058	3.474SerSer: 3.474 ± 0.064	3.105SerThr: 3.105 ± 0.064	4.327SerVal: 4.327 ± 0.069	0.559SerTrp: 0.559 ± 0.023	1.973SerTyr: 1.973 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.65ThrAla: 4.65 ± 0.078	0.317ThrCys: 0.317 ± 0.022	3.026ThrAsp: 3.026 ± 0.067	4.051ThrGlu: 4.051 ± 0.062	2.59ThrPhe: 2.59 ± 0.059	4.58ThrGly: 4.58 ± 0.061	1.08ThrHis: 1.08 ± 0.029	4.44ThrIle: 4.44 ± 0.07	2.928ThrLys: 2.928 ± 0.058	5.111ThrLeu: 5.111 ± 0.071	1.507ThrMet: 1.507 ± 0.037	2.051ThrAsn: 2.051 ± 0.05	2.346ThrPro: 2.346 ± 0.048	1.307ThrGln: 1.307 ± 0.036	2.039ThrArg: 2.039 ± 0.044	3.086ThrSer: 3.086 ± 0.057	3.032ThrThr: 3.032 ± 0.065	4.787ThrVal: 4.787 ± 0.075	0.493ThrTrp: 0.493 ± 0.024	1.896ThrTyr: 1.896 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	5.768ValAla: 5.768 ± 0.082	0.532ValCys: 0.532 ± 0.024	3.88ValAsp: 3.88 ± 0.065	5.354ValGlu: 5.354 ± 0.079	3.107ValPhe: 3.107 ± 0.058	5.02ValGly: 5.02 ± 0.088	1.529ValHis: 1.529 ± 0.042	5.433ValIle: 5.433 ± 0.082	4.278ValLys: 4.278 ± 0.073	7.157ValLeu: 7.157 ± 0.092	2.002ValMet: 2.002 ± 0.046	2.853ValAsn: 2.853 ± 0.057	2.985ValPro: 2.985 ± 0.059	2.534ValGln: 2.534 ± 0.059	3.287ValArg: 3.287 ± 0.063	4.388ValSer: 4.388 ± 0.074	4.315ValThr: 4.315 ± 0.085	5.243ValVal: 5.243 ± 0.09	0.663ValTrp: 0.663 ± 0.024	2.134ValTyr: 2.134 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.624TrpAla: 0.624 ± 0.026	0.059TrpCys: 0.059 ± 0.008	0.495TrpAsp: 0.495 ± 0.025	0.639TrpGlu: 0.639 ± 0.027	0.449TrpPhe: 0.449 ± 0.022	0.707TrpGly: 0.707 ± 0.028	0.181TrpHis: 0.181 ± 0.015	0.777TrpIle: 0.777 ± 0.026	0.731TrpLys: 0.731 ± 0.03	1.102TrpLeu: 1.102 ± 0.041	0.342TrpMet: 0.342 ± 0.017	0.502TrpAsn: 0.502 ± 0.022	0.279TrpPro: 0.279 ± 0.017	0.364TrpGln: 0.364 ± 0.02	0.43TrpArg: 0.43 ± 0.019	0.596TrpSer: 0.596 ± 0.026	0.594TrpThr: 0.594 ± 0.026	0.561TrpVal: 0.561 ± 0.024	0.112TrpTrp: 0.112 ± 0.011	0.278TrpTyr: 0.278 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.327TyrAla: 2.327 ± 0.054	0.277TyrCys: 0.277 ± 0.019	1.89TyrAsp: 1.89 ± 0.044	2.591TyrGlu: 2.591 ± 0.05	1.538TyrPhe: 1.538 ± 0.043	2.489TyrGly: 2.489 ± 0.05	0.664TyrHis: 0.664 ± 0.024	2.094TyrIle: 2.094 ± 0.046	1.609TyrLys: 1.609 ± 0.04	3.058TyrLeu: 3.058 ± 0.057	0.82TyrMet: 0.82 ± 0.029	1.092TyrAsn: 1.092 ± 0.035	1.338TyrPro: 1.338 ± 0.038	1.139TyrGln: 1.139 ± 0.038	1.671TyrArg: 1.671 ± 0.041	1.88TyrSer: 1.88 ± 0.044	1.696TyrThr: 1.696 ± 0.039	2.167TyrVal: 2.167 ± 0.044	0.35TyrTrp: 0.35 ± 0.018	1.192TyrTyr: 1.192 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3544 proteins (1011903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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