Tuhoko virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Pararubulavirus; Tuhoko pararubulavirus 2

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8WJ30|D8WJ30_9MONO Phosphoprotein OS=Tuhoko virus 2 OX=798073 GN=V/P PE=3 SV=1
MM1 pKa = 7.48SSVFQAFEE9 pKa = 4.07QFTLEE14 pKa = 3.65QDD16 pKa = 3.61QQVGGGNLEE25 pKa = 4.19VPPEE29 pKa = 4.04TLQTTIKK36 pKa = 10.68VFILNTQNPQIRR48 pKa = 11.84YY49 pKa = 9.52QMLCFCLRR57 pKa = 11.84IIASNSARR65 pKa = 11.84TAQKK69 pKa = 10.34HH70 pKa = 4.8GALLTILSLPTAMMQNHH87 pKa = 5.88IRR89 pKa = 11.84IADD92 pKa = 4.23RR93 pKa = 11.84SPDD96 pKa = 3.55SVIEE100 pKa = 4.4RR101 pKa = 11.84IEE103 pKa = 3.87IEE105 pKa = 4.1GFEE108 pKa = 4.41PGTYY112 pKa = 9.72RR113 pKa = 11.84LRR115 pKa = 11.84ANARR119 pKa = 11.84TPMTAGEE126 pKa = 4.39VIALEE131 pKa = 4.13NMAEE135 pKa = 4.24DD136 pKa = 5.5LPDD139 pKa = 3.44TLANQTPFLNPNTEE153 pKa = 4.97DD154 pKa = 3.96EE155 pKa = 4.67ICDD158 pKa = 3.68EE159 pKa = 4.22MEE161 pKa = 4.09KK162 pKa = 10.78FLDD165 pKa = 3.45AVYY168 pKa = 10.83SVLIQVWVMVCKK180 pKa = 10.77AMTAFDD186 pKa = 4.13QPTGSDD192 pKa = 2.96TRR194 pKa = 11.84RR195 pKa = 11.84LAKK198 pKa = 10.1YY199 pKa = 7.72QQQGRR204 pKa = 11.84MDD206 pKa = 3.61QKK208 pKa = 11.24YY209 pKa = 10.1LLQNEE214 pKa = 4.29VRR216 pKa = 11.84RR217 pKa = 11.84LIQLGIRR224 pKa = 11.84EE225 pKa = 4.55SLTIRR230 pKa = 11.84QFLVFEE236 pKa = 4.25MQTARR241 pKa = 11.84RR242 pKa = 11.84QGPITSKK249 pKa = 9.45YY250 pKa = 8.55YY251 pKa = 11.74AMIGDD256 pKa = 3.91IARR259 pKa = 11.84YY260 pKa = 9.21IEE262 pKa = 4.13NAGMGPFFMTIKK274 pKa = 10.32FALGTRR280 pKa = 11.84WPPLALAAFSGEE292 pKa = 4.01LVKK295 pKa = 10.85IKK297 pKa = 10.93GLMQLYY303 pKa = 9.93RR304 pKa = 11.84RR305 pKa = 11.84LGDD308 pKa = 3.29RR309 pKa = 11.84AKK311 pKa = 11.15YY312 pKa = 8.54MALLEE317 pKa = 4.21MSEE320 pKa = 4.12XMDD323 pKa = 4.67FAPANYY329 pKa = 9.71QLLFSYY335 pKa = 11.03AMGIGRR341 pKa = 11.84VQDD344 pKa = 3.84PQLQGYY350 pKa = 8.67NFARR354 pKa = 11.84PFLNAAFFQLGMEE367 pKa = 4.37TANRR371 pKa = 11.84QQGSVDD377 pKa = 3.22RR378 pKa = 11.84EE379 pKa = 3.97MAEE382 pKa = 4.08EE383 pKa = 4.8LGLTDD388 pKa = 3.67ADD390 pKa = 3.89KK391 pKa = 11.59RR392 pKa = 11.84EE393 pKa = 4.09MAAQLTRR400 pKa = 11.84LTTGRR405 pKa = 11.84GGDD408 pKa = 3.71DD409 pKa = 2.95QQGIVNVFGRR419 pKa = 11.84RR420 pKa = 11.84AQRR423 pKa = 11.84QQVIQNQDD431 pKa = 2.63QFRR434 pKa = 11.84VVEE437 pKa = 4.16EE438 pKa = 4.31EE439 pKa = 3.8EE440 pKa = 4.36SEE442 pKa = 4.23EE443 pKa = 3.95EE444 pKa = 3.95EE445 pKa = 4.79EE446 pKa = 4.53EE447 pKa = 4.77IEE449 pKa = 4.14EE450 pKa = 4.18QPVRR454 pKa = 11.84GAAPAPIRR462 pKa = 11.84RR463 pKa = 11.84PNDD466 pKa = 3.07EE467 pKa = 3.64EE468 pKa = 4.45AAWQARR474 pKa = 11.84LNEE477 pKa = 4.12LEE479 pKa = 3.99GMQRR483 pKa = 11.84RR484 pKa = 11.84PHH486 pKa = 6.7PPRR489 pKa = 11.84HH490 pKa = 5.34VQLHH494 pKa = 6.23PLQPVPVGLAAAQIHH509 pKa = 6.29RR510 pKa = 11.84EE511 pKa = 3.98PPAQEE516 pKa = 4.05LVADD520 pKa = 4.47LVEE523 pKa = 4.12

Molecular weight: 59.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8WJ33|D8WJ33_9MONO Fusion glycoprotein F0 OS=Tuhoko virus 2 OX=798073 GN=F PE=3 SV=1
MM1 pKa = 7.76AHH3 pKa = 6.39RR4 pKa = 11.84QATVSIKK11 pKa = 10.54VNHH14 pKa = 6.07EE15 pKa = 3.85SEE17 pKa = 4.53KK18 pKa = 9.89NHH20 pKa = 6.12LRR22 pKa = 11.84PFPIVQLPPSPGGSRR37 pKa = 11.84GRR39 pKa = 11.84LTRR42 pKa = 11.84QIRR45 pKa = 11.84IKK47 pKa = 10.74DD48 pKa = 3.6LTPAGSTEE56 pKa = 3.91PSLTFINTYY65 pKa = 10.58GFAKK69 pKa = 10.18PLRR72 pKa = 11.84TRR74 pKa = 11.84TEE76 pKa = 4.15FFSEE80 pKa = 3.77LHH82 pKa = 6.59KK83 pKa = 10.64PDD85 pKa = 3.98SLPSVTACTLPFGAGPNIEE104 pKa = 4.27HH105 pKa = 7.61PMRR108 pKa = 11.84LLDD111 pKa = 4.75EE112 pKa = 4.39IEE114 pKa = 4.14KK115 pKa = 10.19VHH117 pKa = 7.59IVMRR121 pKa = 11.84KK122 pKa = 7.68SASARR127 pKa = 11.84EE128 pKa = 3.87EE129 pKa = 3.73IVFNVCRR136 pKa = 11.84LPPILSKK143 pKa = 10.77HH144 pKa = 6.29HH145 pKa = 6.72IATQKK150 pKa = 10.19LVCISSEE157 pKa = 4.34KK158 pKa = 9.82YY159 pKa = 10.35LKK161 pKa = 10.93APGKK165 pKa = 9.23MISGMDD171 pKa = 3.79YY172 pKa = 9.66NYY174 pKa = 10.24HH175 pKa = 5.96IAFISVVYY183 pKa = 10.16CPASLKK189 pKa = 10.19FRR191 pKa = 11.84VMRR194 pKa = 11.84PLQLLRR200 pKa = 11.84SSTMRR205 pKa = 11.84SIQLEE210 pKa = 4.42VILVIEE216 pKa = 4.9CSDD219 pKa = 3.48NSPATRR225 pKa = 11.84NLIFDD230 pKa = 4.98DD231 pKa = 4.89EE232 pKa = 5.04SKK234 pKa = 8.87TWRR237 pKa = 11.84ASVWFHH243 pKa = 6.53LCNILKK249 pKa = 10.31SNKK252 pKa = 9.47SAEE255 pKa = 4.41KK256 pKa = 10.87YY257 pKa = 9.83DD258 pKa = 3.36DD259 pKa = 4.2HH260 pKa = 8.34YY261 pKa = 11.87FNEE264 pKa = 4.53KK265 pKa = 8.69CKK267 pKa = 10.89KK268 pKa = 8.52MDD270 pKa = 4.06LEE272 pKa = 4.46VGIADD277 pKa = 3.19MWGPTFLVKK286 pKa = 10.59AHH288 pKa = 5.9GKK290 pKa = 8.94IPKK293 pKa = 8.06TAQVYY298 pKa = 7.28FSPKK302 pKa = 9.38GWSCHH307 pKa = 5.53PLVDD311 pKa = 4.92AAPALSKK318 pKa = 10.7ILWSTGARR326 pKa = 11.84IVNVNAILQPSDD338 pKa = 3.52LGQLVQVSDD347 pKa = 3.81VIYY350 pKa = 9.81PKK352 pKa = 10.98VKK354 pKa = 10.12INKK357 pKa = 9.27KK358 pKa = 10.27LMNTAPSRR366 pKa = 11.84WNPVKK371 pKa = 10.6KK372 pKa = 10.45AVLAA376 pKa = 4.44

Molecular weight: 42.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

4935

241

2267

705.0

79.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.856 ± 0.671

2.006 ± 0.231

5.187 ± 0.462

5.593 ± 0.724

3.465 ± 0.229

5.046 ± 0.514

1.803 ± 0.214

8.024 ± 0.791

5.471 ± 0.555

10.902 ± 1.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.25

4.985 ± 0.46

5.431 ± 0.707

5.187 ± 0.751

4.965 ± 0.585

8.065 ± 0.65

5.876 ± 0.303

5.512 ± 0.522

0.952 ± 0.204

3.161 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski