Blueberry necrotic ring blotch virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Kitaviridae; Blunervirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5DFD0|G5DFD0_9VIRU p24 OS=Blueberry necrotic ring blotch virus OX=1094249 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 5.91LNTVINCILLTSSFFSPVEE21 pKa = 3.78VRR23 pKa = 11.84KK24 pKa = 10.31CPEE27 pKa = 3.49FTLEE31 pKa = 4.05SKK33 pKa = 10.5RR34 pKa = 11.84VSHH37 pKa = 6.98HH38 pKa = 5.68FFRR41 pKa = 11.84NNLYY45 pKa = 10.28LYY47 pKa = 10.14KK48 pKa = 10.41PKK50 pKa = 10.6LPRR53 pKa = 11.84LLTYY57 pKa = 10.71KK58 pKa = 8.49FTPYY62 pKa = 10.76CEE64 pKa = 3.36ICARR68 pKa = 11.84KK69 pKa = 9.7ISDD72 pKa = 3.63YY73 pKa = 11.12EE74 pKa = 4.0YY75 pKa = 11.21DD76 pKa = 4.16DD77 pKa = 6.5DD78 pKa = 6.05ILILPLGNLCRR89 pKa = 11.84NCKK92 pKa = 10.18SIPPSDD98 pKa = 3.64DD99 pKa = 3.1TFGPVYY105 pKa = 10.36NFSFVVPTPSQHH117 pKa = 6.29CPASSEE123 pKa = 4.54VYY125 pKa = 10.48NVTSDD130 pKa = 2.87GSYY133 pKa = 10.89ILLHH137 pKa = 6.76RR138 pKa = 11.84NTAFSKK144 pKa = 9.61EE145 pKa = 3.66FRR147 pKa = 11.84YY148 pKa = 8.5TISKK152 pKa = 9.97CHH154 pKa = 6.68DD155 pKa = 3.96PYY157 pKa = 11.05ILLHH161 pKa = 5.21ITEE164 pKa = 5.11LGFVDD169 pKa = 5.82EE170 pKa = 4.52ILDD173 pKa = 4.78RR174 pKa = 11.84ITSSIGTIFRR184 pKa = 11.84TIVTEE189 pKa = 3.86LTHH192 pKa = 7.34IFGGPIHH199 pKa = 6.83AVLVTIPHH207 pKa = 6.75AFLSLVHH214 pKa = 6.29YY215 pKa = 9.33ISSVIEE221 pKa = 3.83YY222 pKa = 10.8LLFDD226 pKa = 3.77VGLANALLITIAFWFKK242 pKa = 10.26FPWMQATLCALLVIYY257 pKa = 7.2VTKK260 pKa = 10.98LNVLFHH266 pKa = 6.85

Molecular weight: 30.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5DFC9|G5DFC9_9VIRU Uncharacterized protein OS=Blueberry necrotic ring blotch virus OX=1094249 PE=4 SV=1
MM1 pKa = 7.69AYY3 pKa = 9.94VDD5 pKa = 3.83RR6 pKa = 11.84VVVTTVTPSASVHH19 pKa = 4.16RR20 pKa = 11.84TVYY23 pKa = 9.92SVHH26 pKa = 5.91VNSVVSSVDD35 pKa = 3.1TRR37 pKa = 11.84IGDD40 pKa = 3.74IFPSPEE46 pKa = 3.61RR47 pKa = 11.84YY48 pKa = 7.82TRR50 pKa = 11.84YY51 pKa = 8.5TISKK55 pKa = 7.73GARR58 pKa = 11.84WW59 pKa = 3.31

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4128

59

1887

589.7

66.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.475 ± 0.509

2.083 ± 0.134

5.911 ± 0.414

4.918 ± 0.36

5.136 ± 0.43

4.312 ± 0.287

3.173 ± 0.253

5.911 ± 0.445

5.354 ± 0.416

9.084 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.011 ± 0.149

4.675 ± 0.334

5.281 ± 0.809

2.762 ± 0.233

5.354 ± 0.249

8.309 ± 0.522

5.959 ± 0.362

9.157 ± 0.716

0.727 ± 0.104

4.409 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski