Mythimna unipuncta granulovirus B
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5YE08|A0A1S5YE08_9BBAC VLF-1 OS=Mythimna unipuncta granulovirus B OX=2169746 PE=3 SV=1
MM1 pKa = 6.76 QHH3 pKa = 6.96 LDD5 pKa = 3.19 IPLEE9 pKa = 4.1 RR10 pKa = 11.84 WSVPDD15 pKa = 3.5 VVDD18 pKa = 6.14 AIPLKK23 pKa = 10.64 LAYY26 pKa = 9.0 TSSKK30 pKa = 10.15 DD31 pKa = 3.37 TTPPSAQAVYY41 pKa = 10.71 NSKK44 pKa = 11.09 DD45 pKa = 3.56 DD46 pKa = 4.58 DD47 pKa = 4.25 AVSPPPATTDD57 pKa = 3.29 TNVWFIAFACITVLAIVLLISYY79 pKa = 7.75 YY80 pKa = 9.84 IVSALRR86 pKa = 11.84 IDD88 pKa = 4.07 DD89 pKa = 3.75 TAEE92 pKa = 4.1 PRR94 pKa = 11.84 DD95 pKa = 3.66 LDD97 pKa = 3.87 YY98 pKa = 11.88 DD99 pKa = 3.87 EE100 pKa = 5.33 LAVYY104 pKa = 9.95
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.63
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.859
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.291
Thurlkill 3.706
EMBOSS 3.872
Sillero 3.986
Patrickios 1.939
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A1S5YDY3|A0A1S5YDY3_9BBAC ORF63 OS=Mythimna unipuncta granulovirus B OX=2169746 PE=4 SV=1
MM1 pKa = 8.09 LFMLCSRR8 pKa = 11.84 FSLHH12 pKa = 7.0 ASSTLVHH19 pKa = 7.02 RR20 pKa = 11.84 SQPSLIDD27 pKa = 2.98 IKK29 pKa = 10.44 TCGKK33 pKa = 9.48 HH34 pKa = 5.57 RR35 pKa = 11.84 NHH37 pKa = 7.68 RR38 pKa = 11.84 YY39 pKa = 7.13 TSSSVSRR46 pKa = 11.84 VVRR49 pKa = 11.84 PEE51 pKa = 3.46 SAKK54 pKa = 10.0 RR55 pKa = 11.84 HH56 pKa = 5.72 AIRR59 pKa = 11.84 LRR61 pKa = 11.84 VRR63 pKa = 11.84 LKK65 pKa = 10.63 RR66 pKa = 11.84 EE67 pKa = 3.3 KK68 pKa = 9.9 ATAISIRR75 pKa = 11.84 PFSRR79 pKa = 11.84 WWRR82 pKa = 11.84 DD83 pKa = 2.8 RR84 pKa = 11.84 SDD86 pKa = 2.85 GGRR89 pKa = 11.84 RR90 pKa = 11.84 VWGRR94 pKa = 11.84 AFDD97 pKa = 3.89 TQRR100 pKa = 11.84 KK101 pKa = 8.64 RR102 pKa = 11.84 LLFVDD107 pKa = 4.3 CVDD110 pKa = 4.39 GFLHH114 pKa = 6.69 ALWRR118 pKa = 11.84 NYY120 pKa = 9.91 RR121 pKa = 11.84 ATEE124 pKa = 3.57 IGALCRR130 pKa = 11.84 TIQEE134 pKa = 4.29 KK135 pKa = 10.62 VSIYY139 pKa = 10.69 KK140 pKa = 10.37 KK141 pKa = 9.65 IVSITKK147 pKa = 8.16 IQNYY151 pKa = 8.74 KK152 pKa = 10.32 KK153 pKa = 10.34 SAACGGIEE161 pKa = 3.56 PGARR165 pKa = 11.84 HH166 pKa = 5.7 RR167 pKa = 11.84 QSCEE171 pKa = 3.33 IFSHH175 pKa = 6.71 HH176 pKa = 7.01 RR177 pKa = 11.84 LQNYY181 pKa = 8.52 PSEE184 pKa = 4.12 IAVAFLRR191 pKa = 11.84 LQVTFYY197 pKa = 11.17 ASCCALVCVYY207 pKa = 10.0 YY208 pKa = 10.84 LKK210 pKa = 10.69 QINLKK215 pKa = 9.77 II216 pKa = 3.84
Molecular weight: 25.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.78
IPC_protein 10.613
Toseland 10.73
ProMoST 10.452
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.052
Grimsley 10.877
Solomon 10.95
Lehninger 10.906
Nozaki 10.745
DTASelect 10.555
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.774
Patrickios 10.745
IPC_peptide 10.95
IPC2_peptide 9.809
IPC2.peptide.svr19 8.452
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
153
0
153
42765
59
1507
279.5
32.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.572 ± 0.16
2.359 ± 0.148
6.468 ± 0.142
5.631 ± 0.209
4.394 ± 0.156
3.952 ± 0.167
2.708 ± 0.144
5.163 ± 0.14
5.617 ± 0.253
9.41 ± 0.178
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.579 ± 0.09
5.989 ± 0.144
4.415 ± 0.317
3.564 ± 0.108
6.012 ± 0.166
6.281 ± 0.152
6.138 ± 0.258
8.175 ± 0.181
0.898 ± 0.059
4.677 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here