Streptococcus satellite phage Javan409

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLX9|A0A4D5ZLX9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan409 OX=2558685 GN=JavanS409_0008 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 9.64TLSDD6 pKa = 3.59TPKK9 pKa = 9.76TFTFTTTLPNFEE21 pKa = 4.31QAQKK25 pKa = 10.83AGSALIGYY33 pKa = 7.54MIGTYY38 pKa = 7.49EE39 pKa = 4.14QSAIDD44 pKa = 3.23ITYY47 pKa = 8.68TGNSIQVDD55 pKa = 4.25YY56 pKa = 11.26ISDD59 pKa = 3.48EE60 pKa = 4.16DD61 pKa = 3.76LAEE64 pKa = 4.17SFEE67 pKa = 5.17RR68 pKa = 11.84ITNSLDD74 pKa = 3.36HH75 pKa = 7.11LDD77 pKa = 5.61DD78 pKa = 4.59YY79 pKa = 11.99DD80 pKa = 6.3DD81 pKa = 5.09YY82 pKa = 12.1DD83 pKa = 5.26DD84 pKa = 6.78FEE86 pKa = 7.45DD87 pKa = 6.1DD88 pKa = 5.33DD89 pKa = 4.07MTIKK93 pKa = 10.93GDD95 pKa = 3.73LDD97 pKa = 3.66NYY99 pKa = 8.62TALIGSFDD107 pKa = 3.9TLGEE111 pKa = 4.09AQAFTEE117 pKa = 4.44NLDD120 pKa = 3.58NDD122 pKa = 4.14LTNGNNFIFEE132 pKa = 4.34QLPDD136 pKa = 3.68MVVLYY141 pKa = 8.61VSPQDD146 pKa = 3.9TIASSTIDD154 pKa = 3.69KK155 pKa = 10.79LIEE158 pKa = 4.31AYY160 pKa = 10.22VKK162 pKa = 11.05YY163 pKa = 9.73EE164 pKa = 3.99AEE166 pKa = 4.2YY167 pKa = 10.95KK168 pKa = 10.45PDD170 pKa = 3.62TFKK173 pKa = 11.38LNYY176 pKa = 9.39HH177 pKa = 5.95GMRR180 pKa = 11.84VSQLQALDD188 pKa = 3.9KK189 pKa = 10.26DD190 pKa = 4.2TLISDD195 pKa = 4.18IIAYY199 pKa = 9.24EE200 pKa = 4.46LEE202 pKa = 4.52LLDD205 pKa = 4.44HH206 pKa = 7.54ADD208 pKa = 3.89RR209 pKa = 11.84LLSDD213 pKa = 4.22EE214 pKa = 5.14PLPLDD219 pKa = 4.13DD220 pKa = 3.48QHH222 pKa = 8.88AFEE225 pKa = 4.97TIEE228 pKa = 4.21LLGDD232 pKa = 3.61DD233 pKa = 4.78VINLVKK239 pKa = 10.56EE240 pKa = 3.81LDD242 pKa = 3.75TNKK245 pKa = 10.01EE246 pKa = 3.34FDD248 pKa = 4.86GIHH251 pKa = 6.49SYY253 pKa = 10.41IISDD257 pKa = 3.45

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMX9|A0A4D5ZMX9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan409 OX=2558685 GN=JavanS409_0004 PE=4 SV=1
MM1 pKa = 7.52SNYY4 pKa = 10.02KK5 pKa = 8.6VTQIYY10 pKa = 10.32RR11 pKa = 11.84DD12 pKa = 3.95DD13 pKa = 4.07KK14 pKa = 11.35STITKK19 pKa = 10.14NKK21 pKa = 10.2AEE23 pKa = 4.71AIEE26 pKa = 4.21KK27 pKa = 9.53KK28 pKa = 10.03KK29 pKa = 9.98KK30 pKa = 9.38HH31 pKa = 5.28EE32 pKa = 3.75RR33 pKa = 11.84VTRR36 pKa = 11.84FKK38 pKa = 10.85RR39 pKa = 11.84IRR41 pKa = 11.84KK42 pKa = 9.08RR43 pKa = 11.84GKK45 pKa = 9.37

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

3384

44

495

141.0

16.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.935 ± 0.465

0.621 ± 0.144

6.708 ± 0.722

8.865 ± 0.78

3.96 ± 0.362

4.196 ± 0.352

1.537 ± 0.198

7.949 ± 0.292

9.722 ± 0.743

10.402 ± 0.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.176

6.531 ± 0.475

3.162 ± 0.385

3.162 ± 0.362

3.753 ± 0.381

5.26 ± 0.44

6.087 ± 0.513

4.521 ± 0.398

0.709 ± 0.162

5.26 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski