Chrysochromulina parva virophage Larry
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P1LUE8|A0A4P1LUE8_9VIRU Tyrosine recombinase OS=Chrysochromulina parva virophage Larry OX=2420056 PE=4 SV=1
MM1 pKa = 7.33 TVPFSTQYY9 pKa = 10.72 YY10 pKa = 8.13 PEE12 pKa = 4.87 PSGGADD18 pKa = 3.22 LTANSVDD25 pKa = 3.14 ASHH28 pKa = 6.94 IVNGSITTADD38 pKa = 3.49 IADD41 pKa = 3.72 HH42 pKa = 6.53 AVTYY46 pKa = 10.73 DD47 pKa = 3.93 KK48 pKa = 10.94 IAVNAVTNTRR58 pKa = 11.84 IANNAVTHH66 pKa = 6.46 AKK68 pKa = 8.76 MASDD72 pKa = 5.25 AIEE75 pKa = 3.82 THH77 pKa = 6.19 NIKK80 pKa = 10.74 DD81 pKa = 4.51 AMVTDD86 pKa = 4.45 AKK88 pKa = 10.5 LAKK91 pKa = 9.56 IKK93 pKa = 10.7 VMLMIHH99 pKa = 6.27 NSVDD103 pKa = 3.28 STVNLNYY110 pKa = 10.43 GVYY113 pKa = 10.56 LRR115 pKa = 11.84 DD116 pKa = 3.56 NNTSVLYY123 pKa = 9.37 FAYY126 pKa = 9.0 PAQPIPPGSSHH137 pKa = 7.03 SLLLNLSSSATIVEE151 pKa = 4.29 VQVDD155 pKa = 3.62 SDD157 pKa = 4.36 GITVVAHH164 pKa = 5.73 EE165 pKa = 4.78 VYY167 pKa = 10.77 SGGTEE172 pKa = 4.32 TEE174 pKa = 4.15 WQDD177 pKa = 3.26 DD178 pKa = 3.93 TLKK181 pKa = 9.81 FTRR184 pKa = 11.84 NAGADD189 pKa = 3.59 NIQIAFGVSDD199 pKa = 3.48 NN200 pKa = 3.98
Molecular weight: 21.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.478
IPC2_protein 4.584
IPC_protein 4.533
Toseland 4.38
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.469
Rodwell 4.393
Grimsley 4.291
Solomon 4.533
Lehninger 4.495
Nozaki 4.66
DTASelect 4.914
Thurlkill 4.418
EMBOSS 4.495
Sillero 4.685
Patrickios 3.376
IPC_peptide 4.533
IPC2_peptide 4.673
IPC2.peptide.svr19 4.609
Protein with the highest isoelectric point:
>tr|A0A499SAH4|A0A499SAH4_9VIRU S3H helicase with TVpol domain OS=Chrysochromulina parva virophage Larry OX=2420056 PE=4 SV=1
MM1 pKa = 7.81 AEE3 pKa = 5.04 DD4 pKa = 4.34 IPTGGWPVRR13 pKa = 11.84 DD14 pKa = 3.41 PFVPKK19 pKa = 10.02 MEE21 pKa = 4.1 EE22 pKa = 3.83 TKK24 pKa = 10.52 DD25 pKa = 3.22 IRR27 pKa = 11.84 DD28 pKa = 3.38 ARR30 pKa = 11.84 SRR32 pKa = 11.84 EE33 pKa = 3.9 MRR35 pKa = 11.84 AMMATPHH42 pKa = 5.43 GRR44 pKa = 11.84 EE45 pKa = 4.02 MIEE48 pKa = 3.55 TRR50 pKa = 11.84 ARR52 pKa = 11.84 QTRR55 pKa = 11.84 AMMATPRR62 pKa = 11.84 GRR64 pKa = 11.84 EE65 pKa = 3.33 EE66 pKa = 3.51 AAARR70 pKa = 11.84 EE71 pKa = 4.02 AAAAAEE77 pKa = 4.27 EE78 pKa = 4.21 EE79 pKa = 4.55 RR80 pKa = 11.84 GAEE83 pKa = 4.08 AARR86 pKa = 11.84 EE87 pKa = 4.14 GQRR90 pKa = 11.84 RR91 pKa = 11.84 VEE93 pKa = 4.3 DD94 pKa = 3.51 YY95 pKa = 10.79 FRR97 pKa = 11.84 EE98 pKa = 3.95 QRR100 pKa = 11.84 AQQLRR105 pKa = 11.84 AEE107 pKa = 4.36 QQRR110 pKa = 11.84 VEE112 pKa = 4.06 QRR114 pKa = 11.84 AEE116 pKa = 3.56 QRR118 pKa = 11.84 MLMRR122 pKa = 11.84 QLPYY126 pKa = 10.69 VEE128 pKa = 4.79 AALGAGPSTSNSDD141 pKa = 3.38 APRR144 pKa = 11.84 PSPEE148 pKa = 3.54 QPARR152 pKa = 11.84 RR153 pKa = 11.84 TGIIPYY159 pKa = 10.0 LSNILNIRR167 pKa = 11.84 QRR169 pKa = 11.84 PRR171 pKa = 11.84 RR172 pKa = 11.84 RR173 pKa = 11.84 NVRR176 pKa = 11.84 PNDD179 pKa = 3.69 DD180 pKa = 3.86 LTGLDD185 pKa = 3.47
Molecular weight: 21.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.092
IPC_protein 10.175
Toseland 10.028
ProMoST 9.97
Dawson 10.248
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.131
Grimsley 10.335
Solomon 10.394
Lehninger 10.35
Nozaki 9.984
DTASelect 10.058
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.175
Patrickios 9.663
IPC_peptide 10.394
IPC2_peptide 9.004
IPC2.peptide.svr19 8.5
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
6851
102
1931
360.6
40.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.736 ± 0.863
0.774 ± 0.284
6.656 ± 0.349
6.014 ± 0.451
3.839 ± 0.494
6.028 ± 0.677
1.825 ± 0.202
6.422 ± 0.643
5.24 ± 1.469
7.371 ± 0.422
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.715 ± 0.186
5.999 ± 0.354
5.693 ± 0.811
3.707 ± 0.309
5.926 ± 0.788
6.262 ± 0.329
7.97 ± 0.642
5.211 ± 0.482
0.613 ± 0.12
3.999 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here