Erwinia phage Midgardsormr38

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F5G3|A0A5Q2F5G3_9CAUD Uncharacterized protein OS=Erwinia phage Midgardsormr38 OX=2663326 PE=4 SV=1
MM1 pKa = 7.61SSLASWSYY9 pKa = 9.18TAQATIWKK17 pKa = 9.16PLGLDD22 pKa = 3.19EE23 pKa = 4.93YY24 pKa = 11.31GDD26 pKa = 3.84YY27 pKa = 11.15LGWSEE32 pKa = 4.72PLVIACDD39 pKa = 3.91YY40 pKa = 11.19QGGLSKK46 pKa = 10.91RR47 pKa = 11.84LGAIGGEE54 pKa = 3.78KK55 pKa = 9.85VVKK58 pKa = 8.71NTIWTEE64 pKa = 3.75YY65 pKa = 10.87ALADD69 pKa = 3.97AGDD72 pKa = 4.3YY73 pKa = 10.82ILIGASSNPDD83 pKa = 4.13PIAAGADD90 pKa = 3.0EE91 pKa = 4.51VMQAIRR97 pKa = 11.84YY98 pKa = 8.73ADD100 pKa = 3.6TFEE103 pKa = 4.0RR104 pKa = 11.84TADD107 pKa = 3.58DD108 pKa = 3.91FAILTGVV115 pKa = 3.11

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F927|A0A5Q2F927_9CAUD NinF family protein OS=Erwinia phage Midgardsormr38 OX=2663326 PE=4 SV=1
MM1 pKa = 6.79TTAQQLIHH9 pKa = 7.12HH10 pKa = 7.57DD11 pKa = 3.22IAQSNRR17 pKa = 11.84IMQRR21 pKa = 11.84YY22 pKa = 7.84YY23 pKa = 10.84KK24 pKa = 10.44SSSLPFTEE32 pKa = 4.13KK33 pKa = 10.17RR34 pKa = 11.84KK35 pKa = 10.07HH36 pKa = 5.7KK37 pKa = 10.68PEE39 pKa = 3.66VCKK42 pKa = 10.64YY43 pKa = 9.24RR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.91RR47 pKa = 11.84VLHH50 pKa = 6.47AIMNRR55 pKa = 11.84EE56 pKa = 3.89MEE58 pKa = 4.21RR59 pKa = 11.84VLRR62 pKa = 11.84IIGAGQPARR71 pKa = 11.84GSKK74 pKa = 10.1

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

15754

30

1136

169.4

18.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.118 ± 0.666

1.314 ± 0.185

5.776 ± 0.216

6.563 ± 0.345

3.231 ± 0.205

7.166 ± 0.315

1.574 ± 0.143

6.252 ± 0.183

5.954 ± 0.271

7.598 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.888 ± 0.171

4.17 ± 0.227

3.478 ± 0.213

4.202 ± 0.373

5.986 ± 0.303

6.722 ± 0.228

6.1 ± 0.318

6.271 ± 0.278

1.46 ± 0.113

3.174 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski