Pelistega ratti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pelistega

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9Y4Q7|A0A6L9Y4Q7_9BURK CvpA family protein OS=Pelistega ratti OX=2652177 GN=F9B74_02435 PE=4 SV=1
MM1 pKa = 7.57SEE3 pKa = 3.6QDD5 pKa = 3.28ALEE8 pKa = 4.1TSFEE12 pKa = 4.23RR13 pKa = 11.84EE14 pKa = 3.39FDD16 pKa = 3.19HH17 pKa = 7.08GFEE20 pKa = 4.26VKK22 pKa = 10.02TIVNQMTLYY31 pKa = 10.34ISFYY35 pKa = 11.02LGDD38 pKa = 3.82TDD40 pKa = 6.03FDD42 pKa = 4.4CLPAIIPASRR52 pKa = 11.84FEE54 pKa = 4.19EE55 pKa = 4.68GFNVHH60 pKa = 6.39VGQLNQQTPDD70 pKa = 3.05IADD73 pKa = 3.37EE74 pKa = 4.31MEE76 pKa = 5.17SILANMDD83 pKa = 3.97DD84 pKa = 3.86NDD86 pKa = 4.08TVVFFCEE93 pKa = 4.12SEE95 pKa = 4.2AEE97 pKa = 4.07IAEE100 pKa = 4.17GLTFLNFSSNDD111 pKa = 3.62HH112 pKa = 6.0ATHH115 pKa = 7.1

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9Y4N9|A0A6L9Y4N9_9BURK Barnase inhibitor OS=Pelistega ratti OX=2652177 GN=F9B74_01200 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1934

0

1934

627191

32

3011

324.3

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.459 ± 0.058

0.844 ± 0.017

5.147 ± 0.051

6.024 ± 0.056

4.193 ± 0.047

6.709 ± 0.061

2.369 ± 0.029

7.364 ± 0.05

5.729 ± 0.054

10.24 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.029

4.308 ± 0.06

4.062 ± 0.035

4.797 ± 0.051

4.612 ± 0.045

5.988 ± 0.033

5.669 ± 0.056

6.617 ± 0.052

1.208 ± 0.022

3.278 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski