Lactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB 947 / NCTC 947) (Levilactobacillus brevis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Levilactobacillus; Levilactobacillus brevis

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q03QF8|Q03QF8_LACBA Uncharacterized protein OS=Lactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB 947 / NCTC 947) OX=387344 GN=LVIS_1467 PE=4 SV=1
MM1 pKa = 7.46FEE3 pKa = 4.53SNHH6 pKa = 5.57ARR8 pKa = 11.84YY9 pKa = 9.64ASYY12 pKa = 8.53GTVAALPGEE21 pKa = 4.57MIDD24 pKa = 5.11AVWQIIDD31 pKa = 5.23DD32 pKa = 4.29DD33 pKa = 4.42LQGLFPLANLLTFKK47 pKa = 10.66LHH49 pKa = 6.19NNQGQTTFEE58 pKa = 4.06YY59 pKa = 11.01VQTDD63 pKa = 3.33DD64 pKa = 4.18DD65 pKa = 4.47QEE67 pKa = 4.17VTLRR71 pKa = 11.84AGFDD75 pKa = 3.35TQFGYY80 pKa = 9.09TPEE83 pKa = 4.5LPATVLAYY91 pKa = 10.35DD92 pKa = 5.06DD93 pKa = 5.0GDD95 pKa = 3.9SQIILLPSEE104 pKa = 4.4TAEE107 pKa = 4.17HH108 pKa = 6.52

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q03NA6|ADDB_LACBA ATP-dependent helicase/deoxyribonuclease subunit B OS=Lactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB 947 / NCTC 947) OX=387344 GN=rexB PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.59KK9 pKa = 7.38RR10 pKa = 11.84HH11 pKa = 5.81RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.12GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2201

0

2201

645933

23

1895

293.5

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.366 ± 0.054

0.447 ± 0.012

5.638 ± 0.055

4.639 ± 0.056

3.993 ± 0.04

6.927 ± 0.05

2.406 ± 0.028

6.131 ± 0.049

5.052 ± 0.065

10.161 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.024

4.098 ± 0.034

3.914 ± 0.029

5.264 ± 0.059

4.568 ± 0.044

5.563 ± 0.056

7.143 ± 0.058

7.417 ± 0.044

1.239 ± 0.023

3.43 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski