Amino acid dipepetide frequency for Bacillus virus Riggi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.661AlaAla: 1.661 ± 0.369
0.532AlaCys: 0.532 ± 0.201
3.522AlaAsp: 3.522 ± 0.531
3.522AlaGlu: 3.522 ± 0.584
3.389AlaPhe: 3.389 ± 0.514
3.854AlaGly: 3.854 ± 0.556
1.13AlaHis: 1.13 ± 0.329
4.917AlaIle: 4.917 ± 0.767
5.582AlaLys: 5.582 ± 0.693
5.648AlaLeu: 5.648 ± 0.873
1.595AlaMet: 1.595 ± 0.469
2.459AlaAsn: 2.459 ± 0.351
1.927AlaPro: 1.927 ± 0.356
1.728AlaGln: 1.728 ± 0.353
2.99AlaArg: 2.99 ± 0.521
3.19AlaSer: 3.19 ± 0.463
3.455AlaThr: 3.455 ± 0.578
4.12AlaVal: 4.12 ± 0.657
0.731AlaTrp: 0.731 ± 0.227
2.724AlaTyr: 2.724 ± 0.315
0.0AlaXaa: 0.0 ± 0.0
Cys
0.598CysAla: 0.598 ± 0.226
0.066CysCys: 0.066 ± 0.066
0.664CysAsp: 0.664 ± 0.223
0.532CysGlu: 0.532 ± 0.173
0.797CysPhe: 0.797 ± 0.249
0.731CysGly: 0.731 ± 0.184
0.598CysHis: 0.598 ± 0.183
0.465CysIle: 0.465 ± 0.181
1.063CysLys: 1.063 ± 0.274
0.93CysLeu: 0.93 ± 0.247
0.133CysMet: 0.133 ± 0.083
0.266CysAsn: 0.266 ± 0.129
0.199CysPro: 0.199 ± 0.126
0.199CysGln: 0.199 ± 0.113
0.199CysArg: 0.199 ± 0.116
0.532CysSer: 0.532 ± 0.187
0.797CysThr: 0.797 ± 0.237
0.399CysVal: 0.399 ± 0.169
0.133CysTrp: 0.133 ± 0.111
0.332CysTyr: 0.332 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
3.522AspAla: 3.522 ± 0.477
0.465AspCys: 0.465 ± 0.19
3.721AspAsp: 3.721 ± 0.456
4.319AspGlu: 4.319 ± 0.697
3.788AspPhe: 3.788 ± 0.602
4.253AspGly: 4.253 ± 0.515
1.595AspHis: 1.595 ± 0.452
4.784AspIle: 4.784 ± 0.54
4.984AspLys: 4.984 ± 0.764
4.917AspLeu: 4.917 ± 0.611
2.06AspMet: 2.06 ± 0.378
2.592AspAsn: 2.592 ± 0.4
2.924AspPro: 2.924 ± 0.466
2.06AspGln: 2.06 ± 0.33
3.655AspArg: 3.655 ± 0.459
2.99AspSer: 2.99 ± 0.429
2.791AspThr: 2.791 ± 0.432
3.522AspVal: 3.522 ± 0.426
0.664AspTrp: 0.664 ± 0.208
2.126AspTyr: 2.126 ± 0.39
0.0AspXaa: 0.0 ± 0.0
Glu
5.382GluAla: 5.382 ± 0.607
0.864GluCys: 0.864 ± 0.2
4.784GluAsp: 4.784 ± 0.625
8.306GluGlu: 8.306 ± 1.166
3.389GluPhe: 3.389 ± 0.578
4.718GluGly: 4.718 ± 0.683
1.927GluHis: 1.927 ± 0.365
4.585GluIle: 4.585 ± 0.598
4.386GluLys: 4.386 ± 0.46
8.107GluLeu: 8.107 ± 0.862
2.326GluMet: 2.326 ± 0.374
3.854GluAsn: 3.854 ± 0.472
1.728GluPro: 1.728 ± 0.416
4.12GluGln: 4.12 ± 0.683
3.123GluArg: 3.123 ± 0.471
3.588GluSer: 3.588 ± 0.55
4.319GluThr: 4.319 ± 0.61
4.452GluVal: 4.452 ± 0.616
1.13GluTrp: 1.13 ± 0.265
3.854GluTyr: 3.854 ± 0.559
0.0GluXaa: 0.0 ± 0.0
Phe
2.525PheAla: 2.525 ± 0.432
0.399PheCys: 0.399 ± 0.18
3.057PheAsp: 3.057 ± 0.521
4.186PheGlu: 4.186 ± 0.469
2.126PhePhe: 2.126 ± 0.464
3.389PheGly: 3.389 ± 0.493
1.196PheHis: 1.196 ± 0.312
2.658PheIle: 2.658 ± 0.406
3.588PheLys: 3.588 ± 0.514
3.389PheLeu: 3.389 ± 0.448
1.462PheMet: 1.462 ± 0.355
2.06PheAsn: 2.06 ± 0.283
1.595PhePro: 1.595 ± 0.333
1.196PheGln: 1.196 ± 0.262
1.595PheArg: 1.595 ± 0.347
2.724PheSer: 2.724 ± 0.555
3.788PheThr: 3.788 ± 0.465
2.724PheVal: 2.724 ± 0.392
0.332PheTrp: 0.332 ± 0.15
1.728PheTyr: 1.728 ± 0.356
0.0PheXaa: 0.0 ± 0.0
Gly
5.05GlyAla: 5.05 ± 0.83
0.664GlyCys: 0.664 ± 0.235
4.519GlyAsp: 4.519 ± 0.585
4.784GlyGlu: 4.784 ± 0.464
3.455GlyPhe: 3.455 ± 0.502
5.117GlyGly: 5.117 ± 0.958
1.13GlyHis: 1.13 ± 0.322
4.784GlyIle: 4.784 ± 0.599
5.98GlyLys: 5.98 ± 0.651
5.25GlyLeu: 5.25 ± 0.659
1.993GlyMet: 1.993 ± 0.425
3.256GlyAsn: 3.256 ± 0.718
0.133GlyPro: 0.133 ± 0.128
2.259GlyGln: 2.259 ± 0.476
2.592GlyArg: 2.592 ± 0.49
4.585GlySer: 4.585 ± 0.557
2.525GlyThr: 2.525 ± 0.382
4.386GlyVal: 4.386 ± 0.587
0.864GlyTrp: 0.864 ± 0.221
3.389GlyTyr: 3.389 ± 0.517
0.0GlyXaa: 0.0 ± 0.0
His
1.13HisAla: 1.13 ± 0.25
0.133HisCys: 0.133 ± 0.079
0.532HisAsp: 0.532 ± 0.203
1.329HisGlu: 1.329 ± 0.371
1.462HisPhe: 1.462 ± 0.265
1.927HisGly: 1.927 ± 0.458
0.399HisHis: 0.399 ± 0.139
1.263HisIle: 1.263 ± 0.247
1.728HisLys: 1.728 ± 0.315
1.861HisLeu: 1.861 ± 0.309
0.664HisMet: 0.664 ± 0.217
1.196HisAsn: 1.196 ± 0.244
1.196HisPro: 1.196 ± 0.239
0.731HisGln: 0.731 ± 0.273
1.196HisArg: 1.196 ± 0.258
1.595HisSer: 1.595 ± 0.36
1.063HisThr: 1.063 ± 0.332
0.864HisVal: 0.864 ± 0.249
0.399HisTrp: 0.399 ± 0.163
1.13HisTyr: 1.13 ± 0.317
0.0HisXaa: 0.0 ± 0.0
Ile
3.522IleAla: 3.522 ± 0.481
0.532IleCys: 0.532 ± 0.22
4.851IleAsp: 4.851 ± 0.606
5.05IleGlu: 5.05 ± 0.479
2.592IlePhe: 2.592 ± 0.565
3.921IleGly: 3.921 ± 0.593
1.395IleHis: 1.395 ± 0.304
4.053IleIle: 4.053 ± 0.631
6.512IleLys: 6.512 ± 0.621
4.452IleLeu: 4.452 ± 0.549
1.794IleMet: 1.794 ± 0.405
3.854IleAsn: 3.854 ± 0.437
2.791IlePro: 2.791 ± 0.422
2.658IleGln: 2.658 ± 0.44
3.057IleArg: 3.057 ± 0.444
4.585IleSer: 4.585 ± 0.686
4.386IleThr: 4.386 ± 0.511
3.987IleVal: 3.987 ± 0.56
0.399IleTrp: 0.399 ± 0.158
2.392IleTyr: 2.392 ± 0.47
0.0IleXaa: 0.0 ± 0.0
Lys
3.987LysAla: 3.987 ± 0.531
0.598LysCys: 0.598 ± 0.222
4.585LysAsp: 4.585 ± 0.64
8.638LysGlu: 8.638 ± 1.047
3.921LysPhe: 3.921 ± 0.685
4.319LysGly: 4.319 ± 0.685
2.193LysHis: 2.193 ± 0.467
4.186LysIle: 4.186 ± 0.52
7.509LysLys: 7.509 ± 0.803
5.848LysLeu: 5.848 ± 0.593
3.123LysMet: 3.123 ± 0.461
4.519LysAsn: 4.519 ± 0.488
3.19LysPro: 3.19 ± 0.459
4.053LysGln: 4.053 ± 0.615
4.319LysArg: 4.319 ± 0.651
4.917LysSer: 4.917 ± 0.723
4.12LysThr: 4.12 ± 0.515
5.382LysVal: 5.382 ± 0.772
0.598LysTrp: 0.598 ± 0.168
3.123LysTyr: 3.123 ± 0.547
0.0LysXaa: 0.0 ± 0.0
Leu
3.721LeuAla: 3.721 ± 0.538
0.731LeuCys: 0.731 ± 0.199
4.917LeuAsp: 4.917 ± 0.637
6.246LeuGlu: 6.246 ± 0.965
2.06LeuPhe: 2.06 ± 0.312
6.246LeuGly: 6.246 ± 0.627
1.794LeuHis: 1.794 ± 0.358
4.851LeuIle: 4.851 ± 0.572
7.908LeuLys: 7.908 ± 0.86
5.848LeuLeu: 5.848 ± 0.676
2.06LeuMet: 2.06 ± 0.399
4.253LeuAsn: 4.253 ± 0.51
3.322LeuPro: 3.322 ± 0.461
2.724LeuGln: 2.724 ± 0.474
3.588LeuArg: 3.588 ± 0.452
5.515LeuSer: 5.515 ± 0.598
5.183LeuThr: 5.183 ± 0.424
5.316LeuVal: 5.316 ± 0.743
0.797LeuTrp: 0.797 ± 0.21
2.592LeuTyr: 2.592 ± 0.444
0.0LeuXaa: 0.0 ± 0.0
Met
2.525MetAla: 2.525 ± 0.448
0.199MetCys: 0.199 ± 0.095
1.794MetAsp: 1.794 ± 0.383
1.861MetGlu: 1.861 ± 0.326
1.329MetPhe: 1.329 ± 0.3
1.395MetGly: 1.395 ± 0.297
0.532MetHis: 0.532 ± 0.2
1.794MetIle: 1.794 ± 0.351
2.924MetLys: 2.924 ± 0.432
1.728MetLeu: 1.728 ± 0.35
0.864MetMet: 0.864 ± 0.227
1.13MetAsn: 1.13 ± 0.225
0.93MetPro: 0.93 ± 0.246
0.465MetGln: 0.465 ± 0.186
1.063MetArg: 1.063 ± 0.231
3.389MetSer: 3.389 ± 0.47
2.392MetThr: 2.392 ± 0.43
1.196MetVal: 1.196 ± 0.261
0.399MetTrp: 0.399 ± 0.185
1.196MetTyr: 1.196 ± 0.317
0.0MetXaa: 0.0 ± 0.0
Asn
2.326AsnAla: 2.326 ± 0.462
0.465AsnCys: 0.465 ± 0.153
3.19AsnAsp: 3.19 ± 0.465
3.455AsnGlu: 3.455 ± 0.458
1.794AsnPhe: 1.794 ± 0.295
3.721AsnGly: 3.721 ± 0.568
1.462AsnHis: 1.462 ± 0.258
3.256AsnIle: 3.256 ± 0.576
4.12AsnLys: 4.12 ± 0.506
3.322AsnLeu: 3.322 ± 0.494
1.329AsnMet: 1.329 ± 0.319
3.522AsnAsn: 3.522 ± 0.537
2.259AsnPro: 2.259 ± 0.415
1.661AsnGln: 1.661 ± 0.306
2.259AsnArg: 2.259 ± 0.386
3.522AsnSer: 3.522 ± 0.498
3.256AsnThr: 3.256 ± 0.887
2.924AsnVal: 2.924 ± 0.421
0.997AsnTrp: 0.997 ± 0.278
1.528AsnTyr: 1.528 ± 0.281
0.0AsnXaa: 0.0 ± 0.0
Pro
3.057ProAla: 3.057 ± 0.472
0.266ProCys: 0.266 ± 0.139
2.326ProAsp: 2.326 ± 0.466
3.057ProGlu: 3.057 ± 0.396
2.259ProPhe: 2.259 ± 0.376
0.0ProGly: 0.0 ± 0.0
0.797ProHis: 0.797 ± 0.216
1.794ProIle: 1.794 ± 0.37
2.592ProLys: 2.592 ± 0.488
3.854ProLeu: 3.854 ± 0.588
1.263ProMet: 1.263 ± 0.301
1.794ProAsn: 1.794 ± 0.358
1.063ProPro: 1.063 ± 0.278
1.063ProGln: 1.063 ± 0.246
1.063ProArg: 1.063 ± 0.296
2.193ProSer: 2.193 ± 0.521
1.927ProThr: 1.927 ± 0.476
1.927ProVal: 1.927 ± 0.285
0.266ProTrp: 0.266 ± 0.116
0.598ProTyr: 0.598 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
1.861GlnAla: 1.861 ± 0.296
0.332GlnCys: 0.332 ± 0.126
1.993GlnAsp: 1.993 ± 0.356
3.854GlnGlu: 3.854 ± 0.477
1.196GlnPhe: 1.196 ± 0.28
2.392GlnGly: 2.392 ± 0.431
0.664GlnHis: 0.664 ± 0.233
2.392GlnIle: 2.392 ± 0.42
2.724GlnLys: 2.724 ± 0.449
3.322GlnLeu: 3.322 ± 0.592
1.063GlnMet: 1.063 ± 0.253
1.329GlnAsn: 1.329 ± 0.299
1.329GlnPro: 1.329 ± 0.277
1.063GlnGln: 1.063 ± 0.231
2.193GlnArg: 2.193 ± 0.421
2.06GlnSer: 2.06 ± 0.37
2.06GlnThr: 2.06 ± 0.379
2.592GlnVal: 2.592 ± 0.398
0.598GlnTrp: 0.598 ± 0.19
1.462GlnTyr: 1.462 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
3.588ArgAla: 3.588 ± 0.524
0.465ArgCys: 0.465 ± 0.179
1.794ArgAsp: 1.794 ± 0.398
4.186ArgGlu: 4.186 ± 0.676
2.126ArgPhe: 2.126 ± 0.378
2.658ArgGly: 2.658 ± 0.402
0.797ArgHis: 0.797 ± 0.247
2.99ArgIle: 2.99 ± 0.427
3.987ArgLys: 3.987 ± 0.516
2.592ArgLeu: 2.592 ± 0.486
1.263ArgMet: 1.263 ± 0.262
2.326ArgAsn: 2.326 ± 0.54
1.196ArgPro: 1.196 ± 0.29
1.861ArgGln: 1.861 ± 0.344
2.99ArgArg: 2.99 ± 0.373
1.794ArgSer: 1.794 ± 0.361
2.658ArgThr: 2.658 ± 0.46
2.857ArgVal: 2.857 ± 0.424
0.598ArgTrp: 0.598 ± 0.228
1.794ArgTyr: 1.794 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
3.788SerAla: 3.788 ± 0.696
0.997SerCys: 0.997 ± 0.325
3.987SerAsp: 3.987 ± 0.704
3.788SerGlu: 3.788 ± 0.539
2.99SerPhe: 2.99 ± 0.413
5.449SerGly: 5.449 ± 0.944
1.462SerHis: 1.462 ± 0.288
4.651SerIle: 4.651 ± 0.612
5.25SerLys: 5.25 ± 0.702
4.851SerLeu: 4.851 ± 0.614
1.661SerMet: 1.661 ± 0.315
4.053SerAsn: 4.053 ± 0.552
1.528SerPro: 1.528 ± 0.361
3.19SerGln: 3.19 ± 0.457
1.595SerArg: 1.595 ± 0.336
4.186SerSer: 4.186 ± 0.811
2.857SerThr: 2.857 ± 0.546
3.322SerVal: 3.322 ± 0.544
0.797SerTrp: 0.797 ± 0.205
2.193SerTyr: 2.193 ± 0.415
0.0SerXaa: 0.0 ± 0.0
Thr
4.253ThrAla: 4.253 ± 0.495
0.664ThrCys: 0.664 ± 0.205
3.721ThrAsp: 3.721 ± 0.524
4.386ThrGlu: 4.386 ± 0.633
3.057ThrPhe: 3.057 ± 0.56
4.784ThrGly: 4.784 ± 0.644
0.997ThrHis: 0.997 ± 0.261
4.984ThrIle: 4.984 ± 0.591
3.588ThrLys: 3.588 ± 0.485
4.053ThrLeu: 4.053 ± 0.603
1.063ThrMet: 1.063 ± 0.273
2.525ThrAsn: 2.525 ± 0.485
3.256ThrPro: 3.256 ± 0.552
1.595ThrGln: 1.595 ± 0.268
2.326ThrArg: 2.326 ± 0.432
3.588ThrSer: 3.588 ± 0.87
3.987ThrThr: 3.987 ± 0.712
4.053ThrVal: 4.053 ± 0.529
0.797ThrTrp: 0.797 ± 0.348
2.06ThrTyr: 2.06 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
4.186ValAla: 4.186 ± 0.685
0.93ValCys: 0.93 ± 0.261
4.12ValAsp: 4.12 ± 0.504
3.455ValGlu: 3.455 ± 0.431
2.525ValPhe: 2.525 ± 0.348
3.788ValGly: 3.788 ± 0.536
0.93ValHis: 0.93 ± 0.226
4.917ValIle: 4.917 ± 0.532
4.851ValLys: 4.851 ± 0.697
4.851ValLeu: 4.851 ± 0.634
1.993ValMet: 1.993 ± 0.29
3.123ValAsn: 3.123 ± 0.409
1.661ValPro: 1.661 ± 0.324
2.658ValGln: 2.658 ± 0.409
2.525ValArg: 2.525 ± 0.393
3.522ValSer: 3.522 ± 0.72
4.253ValThr: 4.253 ± 0.619
3.987ValVal: 3.987 ± 0.75
0.465ValTrp: 0.465 ± 0.181
2.658ValTyr: 2.658 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
0.332TrpAla: 0.332 ± 0.166
0.133TrpCys: 0.133 ± 0.083
0.797TrpAsp: 0.797 ± 0.209
0.664TrpGlu: 0.664 ± 0.22
0.465TrpPhe: 0.465 ± 0.171
0.598TrpGly: 0.598 ± 0.201
0.133TrpHis: 0.133 ± 0.102
0.997TrpIle: 0.997 ± 0.273
0.664TrpLys: 0.664 ± 0.226
1.13TrpLeu: 1.13 ± 0.3
0.399TrpMet: 0.399 ± 0.185
0.797TrpAsn: 0.797 ± 0.211
0.0TrpPro: 0.0 ± 0.0
0.332TrpGln: 0.332 ± 0.117
0.598TrpArg: 0.598 ± 0.229
1.595TrpSer: 1.595 ± 0.438
0.598TrpThr: 0.598 ± 0.229
0.532TrpVal: 0.532 ± 0.225
0.199TrpTrp: 0.199 ± 0.125
0.399TrpTyr: 0.399 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.661TyrAla: 1.661 ± 0.312
0.199TyrCys: 0.199 ± 0.117
2.857TyrAsp: 2.857 ± 0.461
3.322TyrGlu: 3.322 ± 0.413
0.997TyrPhe: 0.997 ± 0.249
3.455TyrGly: 3.455 ± 0.461
0.664TyrHis: 0.664 ± 0.212
2.459TyrIle: 2.459 ± 0.488
3.19TyrLys: 3.19 ± 0.55
3.721TyrLeu: 3.721 ± 0.611
0.93TyrMet: 0.93 ± 0.244
1.528TyrAsn: 1.528 ± 0.315
0.93TyrPro: 0.93 ± 0.279
0.864TyrGln: 0.864 ± 0.225
1.595TyrArg: 1.595 ± 0.428
2.525TyrSer: 2.525 ± 0.385
3.322TyrThr: 3.322 ± 0.511
2.857TyrVal: 2.857 ± 0.508
0.199TyrTrp: 0.199 ± 0.134
1.196TyrTyr: 1.196 ± 0.265
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (15050 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski