Clostridium taeniosporum
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2948 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D7XJ94|A0A1D7XJ94_9CLOT NAD-dependent dehydratase OS=Clostridium taeniosporum OX=394958 GN=BGI42_06490 PE=4 SV=1
MM1 pKa = 7.68 PSILRR6 pKa = 11.84 YY7 pKa = 8.58 STTARR12 pKa = 11.84 GSMVITGNTLGLLGKK27 pKa = 9.53 FNPPDD32 pKa = 3.61 ATDD35 pKa = 3.33 TDD37 pKa = 4.14 VNANGIGAFISLNNGISAPGGYY59 pKa = 9.44 PLGTTFDD66 pKa = 3.37 WRR68 pKa = 11.84 EE69 pKa = 3.87 NGSDD73 pKa = 4.11 AIIDD77 pKa = 3.63 IPVSAKK83 pKa = 9.84 ILYY86 pKa = 10.41 AEE88 pKa = 4.78 LSWSGSYY95 pKa = 11.01 LNADD99 pKa = 3.21 QDD101 pKa = 3.8 VSAFLDD107 pKa = 3.41 NAITFNTPLGSFQVNPDD124 pKa = 3.12 AATAGSVVFPTNGVGQYY141 pKa = 9.62 FRR143 pKa = 11.84 SANVTTLVNSGTGTYY158 pKa = 9.77 SVQSIPGVLGGTPSNPAVEE177 pKa = 4.18 NNYY180 pKa = 10.36 LGWSLEE186 pKa = 3.74 IAYY189 pKa = 10.05 EE190 pKa = 4.31 DD191 pKa = 3.18 VTLPYY196 pKa = 10.94 RR197 pKa = 11.84 NMNVWTIFDD206 pKa = 4.38 PVDD209 pKa = 3.56 LAISPIVDD217 pKa = 3.2 IPVSGFSTPISGSIKK232 pKa = 10.36 ARR234 pKa = 11.84 LLISAGEE241 pKa = 4.22 GDD243 pKa = 3.96 PQVTGDD249 pKa = 3.65 QVLFGPDD256 pKa = 2.77 IVGLVALQGEE266 pKa = 4.86 PPSPPNNPVDD276 pKa = 3.42 NFFVSRR282 pKa = 11.84 INDD285 pKa = 3.64 GNSEE289 pKa = 4.43 SLTVGQVDD297 pKa = 3.78 TLGSFGTLNQPVPTTLAGRR316 pKa = 11.84 LAPGRR321 pKa = 11.84 YY322 pKa = 9.76 GYY324 pKa = 10.68 DD325 pKa = 2.81 ITNVDD330 pKa = 3.56 ASTTLVNSQTNALIRR345 pKa = 11.84 LTTSGDD351 pKa = 3.6 GYY353 pKa = 11.43 VPNLLGLQIDD363 pKa = 4.25 SNSPIISINKK373 pKa = 8.57 SAPATATRR381 pKa = 11.84 GSDD384 pKa = 2.66 ITYY387 pKa = 9.23 TLTVTNTGVIQADD400 pKa = 4.11 TVTVLDD406 pKa = 4.42 PAPTGTTVNPGSVTVQNATGPVVNNSTAATLNVDD440 pKa = 4.58 IGPLLPNQVVTIQYY454 pKa = 7.68 TVSTTGTTPTPVLNSTTANYY474 pKa = 9.36 TFVPIPGGNVITDD487 pKa = 3.76 TSNSNEE493 pKa = 4.4 TITAIANKK501 pKa = 9.0 FVCEE505 pKa = 3.89 PFGYY509 pKa = 9.64 RR510 pKa = 11.84 VAAEE514 pKa = 4.14 STGTNSTLLKK524 pKa = 10.48 LNLVTGEE531 pKa = 4.12 VDD533 pKa = 3.94 VLNPDD538 pKa = 2.53 IGMNINAIGYY548 pKa = 8.52 NVLDD552 pKa = 3.58 NLIYY556 pKa = 10.49 GIEE559 pKa = 4.05 NTTTNLVILDD569 pKa = 3.93 STGTTSVLGPVPNLPLLPSPFYY591 pKa = 10.26 NTGTIDD597 pKa = 3.92 NNGHH601 pKa = 7.2 LYY603 pKa = 10.08 IKK605 pKa = 10.67 SSTDD609 pKa = 3.01 PNYY612 pKa = 10.93 YY613 pKa = 10.29 VVDD616 pKa = 4.23 VNSSSPTFGQLVDD629 pKa = 3.99 PLNGFVLDD637 pKa = 3.96 TAPYY641 pKa = 7.3 GTPINPNMLVADD653 pKa = 3.96 WTVSSVDD660 pKa = 3.13 GNLYY664 pKa = 10.48 GVEE667 pKa = 4.08 NTSGNVVSLNPLTGVVTVLTTSGIPIGAYY696 pKa = 9.17 GAVYY700 pKa = 10.22 SINDD704 pKa = 3.27 QYY706 pKa = 11.59 IYY708 pKa = 10.36 ATNNTTGVIYY718 pKa = 10.41 RR719 pKa = 11.84 IEE721 pKa = 4.15 INGDD725 pKa = 3.03 NATGIVFSSDD735 pKa = 3.12 VPSNNNDD742 pKa = 2.87 GAACLNAEE750 pKa = 4.36 LLIDD754 pKa = 5.3 FGDD757 pKa = 4.52 APDD760 pKa = 4.11 PTPGNGPNDD769 pKa = 3.85 YY770 pKa = 9.78 STLLANNGPRR780 pKa = 11.84 HH781 pKa = 5.55 QIINQLYY788 pKa = 10.34 LGTQVTAEE796 pKa = 3.76 NDD798 pKa = 2.99 AYY800 pKa = 11.07 ANITSDD806 pKa = 3.13 ATGDD810 pKa = 4.45 DD811 pKa = 4.51 IIQGIQDD818 pKa = 4.21 DD819 pKa = 4.71 GLVVPLTPMLSTDD832 pKa = 2.86 TTYY835 pKa = 11.88 ALNVSVTNNTGLPANLYY852 pKa = 9.92 GWIDD856 pKa = 3.78 FNEE859 pKa = 4.33 DD860 pKa = 3.77 GIFQGNEE867 pKa = 3.41 AAPVQIVPSALGLQQFILNFIVPVGVVLQPDD898 pKa = 3.52 HH899 pKa = 6.1 TFVRR903 pKa = 11.84 LRR905 pKa = 11.84 LTTDD909 pKa = 3.36 DD910 pKa = 5.96 LINQNSLPIDD920 pKa = 3.77 EE921 pKa = 5.05 DD922 pKa = 3.79 TRR924 pKa = 11.84 SIGPASDD931 pKa = 4.48 GEE933 pKa = 4.37 VEE935 pKa = 5.3 DD936 pKa = 4.9 YY937 pKa = 11.06 YY938 pKa = 11.37 LTITPVADD946 pKa = 2.95 ISVVKK951 pKa = 9.79 TGAPNPVVVGNTLTYY966 pKa = 10.48 TMVVSNLGPSDD977 pKa = 3.77 AQSVSLTDD985 pKa = 4.01 AVPACILNPVYY996 pKa = 10.52 SVNGGTSNPWPVGGINLGTIASGATPITVTISGTVDD1032 pKa = 3.61 PACTEE1037 pKa = 3.91 SSLVNTATVSSPTPDD1052 pKa = 4.27 PDD1054 pKa = 3.74 PSNNTSTVTTQVNQSADD1071 pKa = 3.0 IKK1073 pKa = 9.93 VVKK1076 pKa = 8.71 TASPSPVVAGQTLTYY1091 pKa = 10.07 TMVVSNLGPSDD1102 pKa = 3.77 AQSVSLTDD1110 pKa = 4.01 AVPACILNPVYY1121 pKa = 10.54 SVNGGASNPWPVGGINLGTIAVGATPITVTISGTVDD1157 pKa = 3.52 PACAEE1162 pKa = 4.02 SSLVNTAIVSSPTPDD1177 pKa = 4.24 PDD1179 pKa = 3.74 PSNNTSTVTTQVNQSADD1196 pKa = 2.96 ISVVKK1201 pKa = 9.65 TGSSNPVVAGNILTYY1216 pKa = 10.79 TMVVSNLGPSDD1227 pKa = 3.77 AQSVSLTDD1235 pKa = 4.01 AVPACILNPVYY1246 pKa = 10.52 SVNGGTSNPWPVGGINLGTIASGATPITVTISGTVDD1282 pKa = 3.61 PACTEE1287 pKa = 3.91 SSLVNTATVSSPTPDD1302 pKa = 4.2 LDD1304 pKa = 4.15 PNNNTSTVTTTVNTLADD1321 pKa = 3.52 VSVVKK1326 pKa = 9.09 TANPINVNRR1335 pKa = 11.84 GDD1337 pKa = 3.66 VVTYY1341 pKa = 8.51 TIVVSNAGPSDD1352 pKa = 3.7 AQSVSLADD1360 pKa = 3.64 VVPSEE1365 pKa = 4.15 LLNPEE1370 pKa = 4.06 FSINNGVSWNIWSNPYY1386 pKa = 10.65 SIGTLTAGTSKK1397 pKa = 9.98 TIFIRR1402 pKa = 11.84 GTISNTAIGTVNNTATVSSSTPDD1425 pKa = 3.61 PNPDD1429 pKa = 3.44 NNTSSASIEE1438 pKa = 4.04 VNYY1441 pKa = 10.79 ADD1443 pKa = 5.44 LVAPGNFVKK1452 pKa = 10.71 AVDD1455 pKa = 3.87 KK1456 pKa = 10.97 QYY1458 pKa = 11.84 ADD1460 pKa = 3.23 IGDD1463 pKa = 4.53 EE1464 pKa = 3.59 ITYY1467 pKa = 8.5 TINVTNTGNTIANNVVITDD1486 pKa = 5.09 PIPNGTSYY1494 pKa = 11.63 VSGSLIASAPVTGTPAGGINVTNGIAPGATLTLSYY1529 pKa = 9.89 KK1530 pKa = 10.5 VKK1532 pKa = 10.12 VIQMPVPNPIPNTASISYY1550 pKa = 8.94 TYY1552 pKa = 10.36 TVDD1555 pKa = 3.47 PNNPNGASGSGDD1567 pKa = 3.34 TNTVFTQVNHH1577 pKa = 6.77 GEE1579 pKa = 4.18 ILPEE1583 pKa = 4.06 NAVKK1587 pKa = 9.41 TVDD1590 pKa = 3.95 TNPTTPGDD1598 pKa = 3.77 IITYY1602 pKa = 7.38 TVSIKK1607 pKa = 9.66 NTGNVPVDD1615 pKa = 3.5 ALVTDD1620 pKa = 4.22 VVPTGTQFVTGTVIINGVSKK1640 pKa = 9.96 PAKK1643 pKa = 9.84 NPNFGIKK1650 pKa = 9.86 IIGIPAGEE1658 pKa = 3.96 IANLSFKK1665 pKa = 11.21 VKK1667 pKa = 10.63 VLDD1670 pKa = 3.97 NAPSTLINNAKK1681 pKa = 9.86 INYY1684 pKa = 9.22 SYY1686 pKa = 11.47 VVDD1689 pKa = 3.9 PALPPVNKK1697 pKa = 10.15 DD1698 pKa = 3.04 VTTNTVNVTVLKK1710 pKa = 10.87 PEE1712 pKa = 3.96 LTLVKK1717 pKa = 10.56 SADD1720 pKa = 3.57 RR1721 pKa = 11.84 TGAVVGDD1728 pKa = 4.05 IIRR1731 pKa = 11.84 YY1732 pKa = 9.84 SITATNTGEE1741 pKa = 4.17 IPLQNVIVKK1750 pKa = 10.36 DD1751 pKa = 3.6 VLAPEE1756 pKa = 4.56 LSYY1759 pKa = 10.95 IGNLTINGVPSGQSILTGVNIEE1781 pKa = 4.48 DD1782 pKa = 3.9 LSIGEE1787 pKa = 4.35 VKK1789 pKa = 10.08 TISFDD1794 pKa = 3.55 AKK1796 pKa = 10.82 VNAIPADD1803 pKa = 3.5 KK1804 pKa = 10.23 TINNTSTATFNYY1816 pKa = 9.27 IVSGKK1821 pKa = 9.47 NFSGNATSNEE1831 pKa = 3.79 EE1832 pKa = 4.19 TIRR1835 pKa = 11.84 VYY1837 pKa = 10.97 NPEE1840 pKa = 3.61 LTMTKK1845 pKa = 9.55 MSNNPIVKK1853 pKa = 10.3 VGDD1856 pKa = 3.53 TFQYY1860 pKa = 10.68 TITAEE1865 pKa = 4.04 NTGDD1869 pKa = 3.68 IIINDD1874 pKa = 4.17 VIVKK1878 pKa = 10.39 DD1879 pKa = 3.86 DD1880 pKa = 4.39 LPPEE1884 pKa = 4.16 FEE1886 pKa = 4.26 VVQIIVNGDD1895 pKa = 3.58 VVNGDD1900 pKa = 3.11 IEE1902 pKa = 4.48 AGINIGNLAIGEE1914 pKa = 4.4 SVDD1917 pKa = 3.25 IVLTIQVLADD1927 pKa = 3.61 LTDD1930 pKa = 3.77 TFQNIATGTGTSITDD1945 pKa = 3.62 PNKK1948 pKa = 10.11 PPVIVEE1954 pKa = 4.41 GEE1956 pKa = 4.34 GEE1958 pKa = 4.18 DD1959 pKa = 4.22 PGVTVYY1965 pKa = 10.77 NPKK1968 pKa = 10.64 LEE1970 pKa = 4.31 LEE1972 pKa = 4.29 KK1973 pKa = 11.09 SVDD1976 pKa = 3.29 KK1977 pKa = 10.74 PYY1979 pKa = 11.05 VIVGDD1984 pKa = 3.9 TVTYY1988 pKa = 9.41 TVVAKK1993 pKa = 9.29 NTGDD1997 pKa = 3.25 IVLGNVDD2004 pKa = 5.07 LDD2006 pKa = 4.36 PIVIYY2011 pKa = 10.75 DD2012 pKa = 4.02 LLGPSLEE2019 pKa = 4.25 FVSGSVTIDD2028 pKa = 4.27 GISDD2032 pKa = 3.87 PLSGIVNGVILGVLNVGEE2050 pKa = 4.53 SKK2052 pKa = 9.78 TVTFKK2057 pKa = 11.47 AKK2059 pKa = 10.3 VLSNDD2064 pKa = 2.65 ISPIEE2069 pKa = 4.1 NTAQATFGYY2078 pKa = 9.31 QIPGDD2083 pKa = 3.84 EE2084 pKa = 4.74 PEE2086 pKa = 4.59 TGNATSNTVQVIPEE2100 pKa = 3.97 IANLEE2105 pKa = 4.06 ILKK2108 pKa = 10.49 EE2109 pKa = 3.92 ADD2111 pKa = 3.22 TNFAVMNDD2119 pKa = 3.64 VINYY2123 pKa = 7.03 TVTVTNTGTLDD2134 pKa = 3.4 ANNVIFTDD2142 pKa = 3.68 EE2143 pKa = 4.57 LPPEE2147 pKa = 4.24 VEE2149 pKa = 5.19 LIDD2152 pKa = 4.79 GSFKK2156 pKa = 10.95 VDD2158 pKa = 4.21 GIGINNVDD2166 pKa = 3.65 LRR2168 pKa = 11.84 QGVNIGTIKK2177 pKa = 9.94 TGEE2180 pKa = 3.95 EE2181 pKa = 3.89 AIIEE2185 pKa = 4.21 YY2186 pKa = 9.27 SVKK2189 pKa = 10.63 VIATNCEE2196 pKa = 3.97 LKK2198 pKa = 10.53 IKK2200 pKa = 10.57 NSAIAKK2206 pKa = 7.4 FQYY2209 pKa = 10.26 RR2210 pKa = 11.84 LPNGTTGTVTTDD2222 pKa = 3.21 PSEE2225 pKa = 4.1 TSTVIVNLGISNFKK2239 pKa = 10.51 QLFIEE2244 pKa = 4.42 EE2245 pKa = 4.2 NLIIPDD2251 pKa = 4.01 EE2252 pKa = 4.64 KK2253 pKa = 11.03 PDD2255 pKa = 3.37 IEE2257 pKa = 5.26 EE2258 pKa = 3.99 INEE2261 pKa = 3.84 VNGDD2265 pKa = 3.55 VEE2267 pKa = 4.59 IIKK2270 pKa = 10.12 HH2271 pKa = 4.96 YY2272 pKa = 10.71 VVEE2275 pKa = 4.33 TPILKK2280 pKa = 10.62 SNEE2283 pKa = 3.76 GQILTGCKK2291 pKa = 10.22 LIIQGVLNEE2300 pKa = 4.26 VVEE2303 pKa = 4.37 YY2304 pKa = 9.16 TALDD2308 pKa = 3.72 SEE2310 pKa = 4.62 QTIHH2314 pKa = 6.23 SAHH2317 pKa = 6.04 YY2318 pKa = 9.62 SIPFSTFIILPEE2330 pKa = 4.0 NYY2332 pKa = 9.7 CYY2334 pKa = 11.05 GNEE2337 pKa = 4.7 DD2338 pKa = 2.94 ITTEE2342 pKa = 4.22 VEE2344 pKa = 4.36 DD2345 pKa = 4.84 IYY2347 pKa = 11.4 FNSLNSRR2354 pKa = 11.84 KK2355 pKa = 9.83 LFTNATILIKK2365 pKa = 10.43 ATVSYY2370 pKa = 10.71 CKK2372 pKa = 10.51
Molecular weight: 247.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.999
Patrickios 1.723
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A1D7XNH1|A0A1D7XNH1_9CLOT HEPN domain-containing protein OS=Clostridium taeniosporum OX=394958 GN=BGI42_13800 PE=4 SV=1
MM1 pKa = 7.17 GRR3 pKa = 11.84 EE4 pKa = 4.13 DD5 pKa = 3.34 NRR7 pKa = 11.84 RR8 pKa = 11.84 SGGRR12 pKa = 11.84 GRR14 pKa = 11.84 RR15 pKa = 11.84 SKK17 pKa = 10.92 RR18 pKa = 11.84 KK19 pKa = 8.67 VCAFCVDD26 pKa = 3.31 KK27 pKa = 11.49 AEE29 pKa = 4.67 SIDD32 pKa = 3.62 YY33 pKa = 10.94 KK34 pKa = 11.04 DD35 pKa = 3.2 INKK38 pKa = 9.06 LRR40 pKa = 11.84 KK41 pKa = 9.21 YY42 pKa = 7.83 VTEE45 pKa = 4.22 RR46 pKa = 11.84 GKK48 pKa = 10.15 ILPRR52 pKa = 11.84 RR53 pKa = 11.84 ISGTCAKK60 pKa = 9.97 HH61 pKa = 5.6 QRR63 pKa = 11.84 QLTDD67 pKa = 4.19 AIKK70 pKa = 10.18 RR71 pKa = 11.84 ARR73 pKa = 11.84 NIALLPFTTEE83 pKa = 3.59
Molecular weight: 9.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.706
IPC_protein 10.54
Toseland 10.965
ProMoST 10.716
Dawson 11.023
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.242
Grimsley 11.052
Solomon 11.199
Lehninger 11.169
Nozaki 10.95
DTASelect 10.73
Thurlkill 10.95
EMBOSS 11.374
Sillero 10.965
Patrickios 10.979
IPC_peptide 11.213
IPC2_peptide 9.867
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2948
0
2948
916512
26
3051
310.9
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.084 ± 0.051
1.233 ± 0.018
5.699 ± 0.033
7.495 ± 0.051
4.366 ± 0.034
6.164 ± 0.045
1.25 ± 0.016
10.383 ± 0.064
9.691 ± 0.051
8.998 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.459 ± 0.019
7.133 ± 0.049
2.605 ± 0.03
2.187 ± 0.019
3.103 ± 0.031
6.325 ± 0.038
4.827 ± 0.049
6.14 ± 0.043
0.672 ± 0.014
4.187 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here