Adelie penguin polyomavirus
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7W5B8|A0A0A7W5B8_9POLY Large T antigen OS=Adelie penguin polyomavirus OX=1590650 PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.64 PEE4 pKa = 4.26 QKK6 pKa = 10.44 VEE8 pKa = 4.58 LIRR11 pKa = 11.84 LLEE14 pKa = 4.05 IPIGASKK21 pKa = 11.38 AEE23 pKa = 3.86 VQAAYY28 pKa = 10.22 RR29 pKa = 11.84 RR30 pKa = 11.84 QALRR34 pKa = 11.84 LHH36 pKa = 6.98 PDD38 pKa = 2.78 KK39 pKa = 11.24 GGCPEE44 pKa = 5.42 KK45 pKa = 10.33 MKK47 pKa = 10.61 QLNALYY53 pKa = 9.83 EE54 pKa = 4.3 LYY56 pKa = 10.16 KK57 pKa = 10.55 KK58 pKa = 10.22 HH59 pKa = 6.97 PEE61 pKa = 4.44 DD62 pKa = 5.16 PLDD65 pKa = 4.03 TDD67 pKa = 3.83 PLYY70 pKa = 11.16 CEE72 pKa = 4.33 EE73 pKa = 5.07 SEE75 pKa = 5.74 LSDD78 pKa = 3.19 TDD80 pKa = 3.77 EE81 pKa = 4.57 EE82 pKa = 4.56 EE83 pKa = 4.77 EE84 pKa = 4.45 PDD86 pKa = 3.34 SAYY89 pKa = 10.75 ASTQATRR96 pKa = 11.84 DD97 pKa = 3.57 TQQQDD102 pKa = 2.99 GTQRR106 pKa = 11.84 DD107 pKa = 3.74 QRR109 pKa = 11.84 EE110 pKa = 4.15 TPGPSNHH117 pKa = 6.54 HH118 pKa = 6.17 VSQEE122 pKa = 4.04 QYY124 pKa = 11.08 DD125 pKa = 3.69 RR126 pKa = 11.84 AYY128 pKa = 11.05 CRR130 pKa = 11.84 LLEE133 pKa = 4.49 LKK135 pKa = 9.89 WSLEE139 pKa = 3.89 DD140 pKa = 3.35 LFRR143 pKa = 11.84 RR144 pKa = 11.84 QSRR147 pKa = 11.84 KK148 pKa = 9.29 RR149 pKa = 11.84 KK150 pKa = 9.36 QNLEE154 pKa = 4.04 PEE156 pKa = 4.47 FLQLKK161 pKa = 9.57 RR162 pKa = 11.84 RR163 pKa = 11.84 YY164 pKa = 10.26 DD165 pKa = 3.63 SVPWDD170 pKa = 3.37 VFNNIFPNN178 pKa = 3.57
Molecular weight: 21.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.99
IPC2_protein 5.029
IPC_protein 4.952
Toseland 4.851
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.054
Wikipedia 4.762
Rodwell 4.825
Grimsley 4.762
Solomon 4.914
Lehninger 4.863
Nozaki 5.029
DTASelect 5.156
Thurlkill 4.838
EMBOSS 4.8
Sillero 5.092
Patrickios 4.101
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.05
Protein with the highest isoelectric point:
>tr|A0A0A7WCK8|A0A0A7WCK8_9POLY VP1 OS=Adelie penguin polyomavirus OX=1590650 PE=3 SV=1
MM1 pKa = 7.52 ALVPYY6 pKa = 10.36 DD7 pKa = 3.16 WGQLLDD13 pKa = 3.8 YY14 pKa = 8.5 EE15 pKa = 4.73 FPGLSFIANTLYY27 pKa = 10.99 DD28 pKa = 4.04 AVHH31 pKa = 6.8 TIDD34 pKa = 3.7 PRR36 pKa = 11.84 TWGPSLVNQLLEE48 pKa = 4.23 RR49 pKa = 11.84 LWYY52 pKa = 9.45 RR53 pKa = 11.84 ARR55 pKa = 11.84 LEE57 pKa = 3.96 IEE59 pKa = 4.22 AEE61 pKa = 4.15 GLRR64 pKa = 11.84 AAGEE68 pKa = 4.08 AAQAAGRR75 pKa = 11.84 GLGDD79 pKa = 3.09 VMARR83 pKa = 11.84 FLEE86 pKa = 4.1 NTRR89 pKa = 11.84 WAVTEE94 pKa = 4.38 TPSNAYY100 pKa = 9.02 EE101 pKa = 4.1 WLQQYY106 pKa = 9.05 YY107 pKa = 10.21 QDD109 pKa = 4.39 LPRR112 pKa = 11.84 MRR114 pKa = 11.84 PVQYY118 pKa = 9.95 RR119 pKa = 11.84 QQARR123 pKa = 11.84 ALGVDD128 pKa = 4.61 LPAQPVEE135 pKa = 4.09 EE136 pKa = 4.85 AMDD139 pKa = 3.83 SGDD142 pKa = 3.62 YY143 pKa = 9.42 VAPYY147 pKa = 8.49 PPPGGAGQRR156 pKa = 11.84 TAPQWLLPLLLGLYY170 pKa = 10.24 GDD172 pKa = 5.6 PNPKK176 pKa = 8.87 WDD178 pKa = 3.39 GGKK181 pKa = 10.05 AKK183 pKa = 10.07 KK184 pKa = 9.9 RR185 pKa = 11.84 RR186 pKa = 11.84 ADD188 pKa = 3.61 PSTQTPNKK196 pKa = 9.16 RR197 pKa = 11.84 RR198 pKa = 11.84 NRR200 pKa = 11.84 GTGGKK205 pKa = 9.68 DD206 pKa = 2.97 RR207 pKa = 11.84 SRR209 pKa = 11.84 QHH211 pKa = 5.92 NRR213 pKa = 11.84 SGMLSAAKK221 pKa = 10.26 NGLPQRR227 pKa = 11.84 RR228 pKa = 11.84 LPRR231 pKa = 11.84 SRR233 pKa = 11.84 KK234 pKa = 9.09
Molecular weight: 26.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.836
IPC2_protein 9.399
IPC_protein 9.853
Toseland 9.78
ProMoST 9.736
Dawson 10.087
Bjellqvist 9.853
Wikipedia 10.335
Rodwell 10.218
Grimsley 10.204
Solomon 10.131
Lehninger 10.072
Nozaki 9.75
DTASelect 9.853
Thurlkill 9.897
EMBOSS 10.218
Sillero 9.999
Patrickios 5.258
IPC_peptide 10.116
IPC2_peptide 8.595
IPC2.peptide.svr19 8.478
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1781
178
660
356.2
39.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.535 ± 1.667
1.909 ± 0.707
6.12 ± 0.565
6.794 ± 0.786
2.246 ± 0.284
7.355 ± 1.226
1.516 ± 0.181
3.537 ± 0.572
5.334 ± 0.873
10.163 ± 0.411
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.302 ± 0.241
3.593 ± 0.177
7.131 ± 0.778
5.446 ± 0.495
6.85 ± 0.755
4.997 ± 0.22
6.008 ± 0.464
5.39 ± 0.624
1.348 ± 0.347
3.425 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here