Arthrobacter woluwensis
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3355 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H4R2H8|A0A1H4R2H8_9MICC Protein N-acetyltransferase RimJ/RimL family OS=Arthrobacter woluwensis OX=156980 GN=SAMN04489745_2451 PE=4 SV=1
MM1 pKa = 7.38 SNIPAEE7 pKa = 4.07 LSYY10 pKa = 10.03 TAEE13 pKa = 4.41 HH14 pKa = 6.42 EE15 pKa = 4.58 WVASTEE21 pKa = 3.79 QDD23 pKa = 3.18 GVVRR27 pKa = 11.84 VGITDD32 pKa = 4.44 FAQDD36 pKa = 3.24 ALGDD40 pKa = 3.66 VVYY43 pKa = 10.65 AQVPEE48 pKa = 4.49 AGTAVTANDD57 pKa = 3.28 VVGEE61 pKa = 4.18 VEE63 pKa = 4.35 STKK66 pKa = 10.71 SVSDD70 pKa = 3.26 IYY72 pKa = 11.46 APVSGEE78 pKa = 3.27 IVARR82 pKa = 11.84 NEE84 pKa = 4.35 SLDD87 pKa = 3.98 ADD89 pKa = 3.98 PALINSDD96 pKa = 3.62 PYY98 pKa = 9.67 GAGWLFEE105 pKa = 3.99 VRR107 pKa = 11.84 IAGADD112 pKa = 3.64 EE113 pKa = 4.44 LSGLLSAEE121 pKa = 4.44 AYY123 pKa = 8.56 GQQVGG128 pKa = 3.53
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.668
IPC_protein 3.605
Toseland 3.414
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.338
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.884
Thurlkill 3.478
EMBOSS 3.528
Sillero 3.732
Patrickios 1.812
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A1H4MBM8|A0A1H4MBM8_9MICC Glycerol-3-phosphate dehydrogenase OS=Arthrobacter woluwensis OX=156980 GN=C6401_12175 PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.61 KK12 pKa = 9.72 IPGAQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GKK24 pKa = 8.59 TFVINKK30 pKa = 8.84 KK31 pKa = 9.15 NPRR34 pKa = 11.84 FKK36 pKa = 10.85 ARR38 pKa = 11.84 QGG40 pKa = 3.28
Molecular weight: 4.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.618
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.34
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.054
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3355
0
3355
1100570
29
2555
328.0
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.856 ± 0.058
0.593 ± 0.01
5.619 ± 0.033
5.787 ± 0.056
3.232 ± 0.028
9.244 ± 0.044
2.08 ± 0.02
4.024 ± 0.029
2.674 ± 0.031
10.412 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.815 ± 0.016
2.265 ± 0.027
5.564 ± 0.038
3.207 ± 0.025
6.787 ± 0.053
5.971 ± 0.038
5.884 ± 0.041
8.51 ± 0.039
1.481 ± 0.018
1.993 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here