Setaria viridis (Green bristlegrass) (Setaria italica subsp. viridis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Panicodae; Paniceae; Cenchrinae; Setaria

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U6U9S9|A0A4U6U9S9_SETVI Uncharacterized protein OS=Setaria viridis OX=4556 GN=SEVIR_6G230750v2 PE=4 SV=1
MM1 pKa = 7.49GSDD4 pKa = 3.52FFVFLTGCFTFGCLGLEE21 pKa = 4.27GGARR25 pKa = 11.84GFLRR29 pKa = 11.84GVAGSLLVGGLSPSDD44 pKa = 3.79GDD46 pKa = 3.59WEE48 pKa = 4.56GVLWSSSLAPLGFYY62 pKa = 10.89GDD64 pKa = 4.59GYY66 pKa = 10.77DD67 pKa = 5.12DD68 pKa = 4.87QGGCDD73 pKa = 4.22GPVGDD78 pKa = 5.19DD79 pKa = 3.91GPRR82 pKa = 11.84GGCAGDD88 pKa = 4.67DD89 pKa = 3.54GLKK92 pKa = 10.38FF93 pKa = 3.58

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U6TPC2|A0A4U6TPC2_SETVI Uncharacterized protein OS=Setaria viridis OX=4556 GN=SEVIR_7G003400v2 PE=4 SV=1
MM1 pKa = 7.85ASRR4 pKa = 11.84SITYY8 pKa = 9.4PGLLLLVVALVIVPSSQAKK27 pKa = 9.64RR28 pKa = 11.84SPPRR32 pKa = 11.84SAPTPAPRR40 pKa = 11.84TAPSPAPRR48 pKa = 11.84SAPTPAPRR56 pKa = 11.84SAPTPAPRR64 pKa = 11.84APQAPSPTPAPTPAPRR80 pKa = 11.84TAPTPAPRR88 pKa = 11.84SAPTPAPRR96 pKa = 11.84APQAPSPTPAPKK108 pKa = 9.98PPPRR112 pKa = 11.84TAPTTAPTPAPRR124 pKa = 11.84SAPTPAPRR132 pKa = 11.84APQAPSPTPAPTPAPTPAPRR152 pKa = 11.84TAPTPAPRR160 pKa = 11.84SAPTPAPRR168 pKa = 11.84APQAPSPTPAPMPAPRR184 pKa = 11.84TAPTLAPRR192 pKa = 11.84SAPTPAPRR200 pKa = 11.84APQAPSPTPAPTPAPRR216 pKa = 11.84TAPISAPTPAPRR228 pKa = 11.84SAPTPTPRR236 pKa = 11.84APQAPSPTPAPTPAPRR252 pKa = 11.84TAPTQAPTPAPRR264 pKa = 11.84SAPTPAPRR272 pKa = 11.84APQVPSPTPAPTPAPRR288 pKa = 11.84TAPTPAPTPAPRR300 pKa = 11.84STPTPAPRR308 pKa = 11.84APQAPSPTPAPTPAPRR324 pKa = 11.84TAPTPAPTPTPRR336 pKa = 11.84SAPTPAPRR344 pKa = 11.84APQVPSPTPTPTPAPRR360 pKa = 11.84TAPTPAPRR368 pKa = 11.84SAPTPAPRR376 pKa = 11.84APQAPSPTPAPTPAPRR392 pKa = 11.84TAPTPAPTPAPRR404 pKa = 11.84SAPTPAPRR412 pKa = 11.84APQAPSPTPTPTPAPRR428 pKa = 11.84TAPTPAPTPAPTPAPRR444 pKa = 11.84SAPTPAPRR452 pKa = 11.84APQAPSPTPAPTPAPRR468 pKa = 11.84TAPTPAPRR476 pKa = 11.84SAPTPAPRR484 pKa = 11.84APQAPSPTPTPTPAPRR500 pKa = 11.84TAPTPAPTPAPTPAPRR516 pKa = 11.84SAPTPAPRR524 pKa = 11.84APQAPSPTPAPTPAPRR540 pKa = 11.84TAPTPAPRR548 pKa = 11.84SAPTPAPRR556 pKa = 11.84APQAPSPTPAPTPAPRR572 pKa = 11.84TAPTPAPRR580 pKa = 11.84SAPTPAPRR588 pKa = 11.84APQAPSPTPAPTPAPRR604 pKa = 11.84TAPTPAPRR612 pKa = 11.84SAPTPAPRR620 pKa = 11.84APQAPSPTPAPTPAPRR636 pKa = 11.84MAPTPASTPAPRR648 pKa = 11.84SAPTPAPRR656 pKa = 11.84ASQAPSPTPAPTPAPRR672 pKa = 11.84MAPTPAPRR680 pKa = 11.84SVPTPAPRR688 pKa = 11.84APLAPSPTPAPTLAPRR704 pKa = 11.84TAPTPAPRR712 pKa = 11.84SAPTLAPRR720 pKa = 11.84APQAPSPRR728 pKa = 11.84PAPTPAPRR736 pKa = 11.84TAPTPAPTPTPRR748 pKa = 11.84SAPSPAPRR756 pKa = 11.84APQVPSPTRR765 pKa = 11.84APTPAPRR772 pKa = 11.84TAPTMAPTPAPRR784 pKa = 11.84SAPTPAPRR792 pKa = 11.84APQAPSPTPAPTPAPRR808 pKa = 11.84TAPTPAPTPAPRR820 pKa = 11.84SAPTPAPRR828 pKa = 11.84APQAPSPTHH837 pKa = 6.79APMLAPRR844 pKa = 11.84TAPTPAPTPTPRR856 pKa = 11.84LAPTPAPRR864 pKa = 11.84APQAPSPTTTPTPAPRR880 pKa = 11.84TAPTPTPRR888 pKa = 11.84SAPTPAPRR896 pKa = 11.84APQAPSPTPAPTQAPRR912 pKa = 11.84TAPTPAPTPAPRR924 pKa = 11.84SAPTPAPRR932 pKa = 11.84APQAPSPTPAPTPAPRR948 pKa = 11.84TAPTPAPTPAPRR960 pKa = 11.84SAPTPAPRR968 pKa = 11.84PPQAPSPTPAPTPASRR984 pKa = 11.84TAPTPSPRR992 pKa = 11.84SAPTPAPRR1000 pKa = 11.84APLAPSPTPAPTPAPRR1016 pKa = 11.84TAPTPAPRR1024 pKa = 11.84SAPTPAPRR1032 pKa = 11.84APQAPSPTPAPTPAPRR1048 pKa = 11.84TAPTPAPRR1056 pKa = 11.84SAPTPTPRR1064 pKa = 11.84APQAPSPTPAPTPAPRR1080 pKa = 11.84MAPTPAPTPAPRR1092 pKa = 11.84SAPTPAPRR1100 pKa = 11.84ASQAPSPTPAPTPAPRR1116 pKa = 11.84MAPTPAPRR1124 pKa = 11.84SVPTPAPRR1132 pKa = 11.84APLAPSPTPAPTLAPRR1148 pKa = 11.84TAPTPAPRR1156 pKa = 11.84SAPTLAPRR1164 pKa = 11.84APQAPSPTPAPTPAPRR1180 pKa = 11.84TAPTPAPTPTPRR1192 pKa = 11.84SAPSPAPRR1200 pKa = 11.84APQVPSPTPAPTPAPRR1216 pKa = 11.84TAPTTAPTPAPRR1228 pKa = 11.84SAPTPAPRR1236 pKa = 11.84APQAPSPTPAPTPAPRR1252 pKa = 11.84TAPTPAPTPTPRR1264 pKa = 11.84SAPTPAPRR1272 pKa = 11.84APQAPSPTPAPMLAPRR1288 pKa = 11.84TAPTPAPTPTPRR1300 pKa = 11.84LAPTPAPRR1308 pKa = 11.84APQAPSPTPTPTPAPRR1324 pKa = 11.84MAPTPAPRR1332 pKa = 11.84SAPTPAPRR1340 pKa = 11.84APQAPSPTPAPTQAPRR1356 pKa = 11.84TAPTPAPTPAPRR1368 pKa = 11.84SAPTPAPRR1376 pKa = 11.84APQAPSPTPAPTPAPRR1392 pKa = 11.84TAPTPAPTPAPRR1404 pKa = 11.84SAPTPTPRR1412 pKa = 11.84PPQAPSPTPAPTPASRR1428 pKa = 11.84TAPTPAPRR1436 pKa = 11.84SAPTPAPRR1444 pKa = 11.84APQAPSPTPAPRR1456 pKa = 11.84TAPTPAPRR1464 pKa = 11.84SAPTPAPRR1472 pKa = 11.84APQAPSPTPAPTPAPRR1488 pKa = 11.84TAPTPAPTPAPRR1500 pKa = 11.84FAPTPAPRR1508 pKa = 11.84APQAPSPTPAPTPAPRR1524 pKa = 11.84TAPTPAPTPAPRR1536 pKa = 11.84SAPTLAPRR1544 pKa = 11.84APQAPSPTPTPTPAPRR1560 pKa = 11.84TAPTPAPTPAPRR1572 pKa = 11.84SAPTPAPRR1580 pKa = 11.84APQAPSPTPAPTPAPRR1596 pKa = 11.84TAPTPAPRR1604 pKa = 11.84SAPTPAPRR1612 pKa = 11.84APQAPSPTPAPTPAPRR1628 pKa = 11.84TAPTPAPRR1636 pKa = 11.84SAPTPAPRR1644 pKa = 11.84APQAPSPTPAPTPAPRR1660 pKa = 11.84TAPTPAPAPAPRR1672 pKa = 11.84SAPTPAPRR1680 pKa = 11.84APQAPSPTPAPTPAPMTAPTPSPRR1704 pKa = 11.84SAPTPAPRR1712 pKa = 11.84APQAPSPTPAPTPAPRR1728 pKa = 11.84TAPTPAPRR1736 pKa = 11.84SAPTPAPRR1744 pKa = 11.84APQAPSPTPAPTPTPRR1760 pKa = 11.84TAPTPAPAPAPRR1772 pKa = 11.84SAPTPAPRR1780 pKa = 11.84APQAPSPTPAPTPAPMTAPTPAPAPAPRR1808 pKa = 11.84SAPTPAPRR1816 pKa = 11.84APQAPSPTPPPTPAPRR1832 pKa = 11.84TAPTLAPTPAPRR1844 pKa = 11.84SAPTPAPRR1852 pKa = 11.84APLVPSPTPAPTPAPRR1868 pKa = 11.84MAPTPAPRR1876 pKa = 11.84SAPTPAPRR1884 pKa = 11.84APQAPSPTRR1893 pKa = 11.84APTPAPRR1900 pKa = 11.84TAPTPAPIAAPRR1912 pKa = 11.84SAPTPAPRR1920 pKa = 11.84APQAPSPTPAPTPVPMLAPTPVPAPTPTPARR1951 pKa = 11.84TPAPTPAPTPTPTSAPTPAPTPVPTPSPTPAPTSAPTPVPTPAPTPVPKK2000 pKa = 9.67PAPMPTLTPPPATPPPPPPPTPPPPPTPVPKK2031 pKa = 9.49PAPMPTLTPPPPTPPPPPPPTPPPPPPPTQSVCPAGFDD2069 pKa = 3.48DD2070 pKa = 5.65SKK2072 pKa = 11.63AFLSAIPVYY2081 pKa = 10.57LKK2083 pKa = 10.91RR2084 pKa = 11.84NILLLIVSKK2093 pKa = 10.93NIADD2097 pKa = 4.24ASPGTSTTKK2106 pKa = 10.38CLCYY2110 pKa = 10.59YY2111 pKa = 8.77STNLYY2116 pKa = 10.66LPIPTPNVKK2125 pKa = 10.41GPIACRR2131 pKa = 11.84RR2132 pKa = 11.84GG2133 pKa = 3.21

Molecular weight: 208.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38120

8394

46514

18246505

29

5148

392.3

43.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.183 ± 0.017

1.974 ± 0.006

5.337 ± 0.008

5.994 ± 0.014

3.655 ± 0.007

7.252 ± 0.013

2.498 ± 0.006

4.383 ± 0.009

4.926 ± 0.011

9.583 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.004

3.524 ± 0.009

5.727 ± 0.015

3.541 ± 0.01

6.337 ± 0.011

8.39 ± 0.013

4.768 ± 0.007

6.659 ± 0.009

1.315 ± 0.004

2.547 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski