Microbacterium phage Ciel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CX79|A0A514CX79_9CAUD Minor tail protein OS=Microbacterium phage Ciel OX=2588493 GN=15 PE=4 SV=1
MM1 pKa = 7.26TLIEE5 pKa = 5.13DD6 pKa = 3.87VLSEE10 pKa = 4.01QIAALRR16 pKa = 11.84ASAGLFVRR24 pKa = 11.84QRR26 pKa = 11.84ADD28 pKa = 2.85ALAAAEE34 pKa = 4.04AAAVQAAGADD44 pKa = 3.39ARR46 pKa = 11.84AAALQAEE53 pKa = 4.52LDD55 pKa = 3.79AYY57 pKa = 10.91RR58 pKa = 11.84EE59 pKa = 4.02LLPAPEE65 pKa = 4.43IPEE68 pKa = 4.26DD69 pKa = 3.87APAVEE74 pKa = 4.85LPADD78 pKa = 3.77EE79 pKa = 4.57TTPPLYY85 pKa = 10.19QQLLTEE91 pKa = 4.46RR92 pKa = 11.84EE93 pKa = 4.51GNN95 pKa = 3.52

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CX97|A0A514CX97_9CAUD Helix-turn-helix DNA binding protein OS=Microbacterium phage Ciel OX=2588493 GN=21 PE=4 SV=1
MM1 pKa = 7.33PTMTAVATNAPLPTNARR18 pKa = 11.84KK19 pKa = 8.15LTAGQVAEE27 pKa = 4.15LFGVRR32 pKa = 11.84VEE34 pKa = 4.62TVRR37 pKa = 11.84RR38 pKa = 11.84WADD41 pKa = 2.99AGKK44 pKa = 10.0IPCTRR49 pKa = 11.84TLGGDD54 pKa = 3.15RR55 pKa = 11.84RR56 pKa = 11.84FDD58 pKa = 3.51PAVVQRR64 pKa = 11.84ILHH67 pKa = 6.18DD68 pKa = 3.48VAAAA72 pKa = 3.54

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5430

60

701

217.2

23.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.849 ± 0.551

0.35 ± 0.128

5.488 ± 0.581

5.414 ± 0.482

3.02 ± 0.265

9.042 ± 0.572

1.51 ± 0.215

4.512 ± 0.349

1.897 ± 0.254

9.466 ± 0.722

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.242

3.002 ± 0.366

5.157 ± 0.286

4.144 ± 0.323

7.44 ± 0.672

5.93 ± 0.414

6.501 ± 0.627

7.551 ± 0.481

1.455 ± 0.201

2.228 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski