Bacillus phage Page

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Pagevirus; Bacillus virus Page

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PVI2|U5PVI2_9CAUD Uncharacterized protein OS=Bacillus phage Page OX=1406786 GN=Page_17 PE=4 SV=1
MM1 pKa = 7.32NLGEE5 pKa = 4.62AKK7 pKa = 10.34AHH9 pKa = 6.22ALALMAEE16 pKa = 4.26YY17 pKa = 10.52SVDD20 pKa = 3.22GLEE23 pKa = 5.27ISDD26 pKa = 5.22AEE28 pKa = 4.07NADD31 pKa = 3.5YY32 pKa = 10.84LKK34 pKa = 10.85RR35 pKa = 11.84MPLFAHH41 pKa = 7.09EE42 pKa = 4.19AQMDD46 pKa = 3.72ISARR50 pKa = 11.84SGVTAVMSFEE60 pKa = 5.43LVKK63 pKa = 10.64QDD65 pKa = 3.65DD66 pKa = 3.49KK67 pKa = 11.41KK68 pKa = 11.28YY69 pKa = 11.28NYY71 pKa = 9.15IALPADD77 pKa = 3.74YY78 pKa = 10.93RR79 pKa = 11.84EE80 pKa = 4.09MKK82 pKa = 9.89YY83 pKa = 10.14VFWNEE88 pKa = 3.37EE89 pKa = 4.35LFTGNYY95 pKa = 10.01SILNNQIRR103 pKa = 11.84FNAGFDD109 pKa = 3.67GTIDD113 pKa = 3.92LYY115 pKa = 9.91YY116 pKa = 10.12WKK118 pKa = 10.42YY119 pKa = 8.95PQQLTTQTDD128 pKa = 3.41DD129 pKa = 3.35NYY131 pKa = 10.65EE132 pKa = 3.94FEE134 pKa = 4.41IDD136 pKa = 3.61RR137 pKa = 11.84EE138 pKa = 4.24YY139 pKa = 11.32HH140 pKa = 6.37HH141 pKa = 7.26LIPYY145 pKa = 9.22YY146 pKa = 10.75LGGKK150 pKa = 9.43CMQDD154 pKa = 3.15EE155 pKa = 4.35NQDD158 pKa = 3.02IAEE161 pKa = 4.59RR162 pKa = 11.84LLSDD166 pKa = 3.7YY167 pKa = 11.02YY168 pKa = 11.67NRR170 pKa = 11.84LSDD173 pKa = 3.48VKK175 pKa = 10.15EE176 pKa = 3.77AHH178 pKa = 6.97EE179 pKa = 5.11DD180 pKa = 3.5GQEE183 pKa = 4.06RR184 pKa = 11.84IDD186 pKa = 4.04NIFTIFF192 pKa = 3.35

Molecular weight: 22.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PVY5|U5PVY5_9CAUD Endolysin OS=Bacillus phage Page OX=1406786 GN=Page_24 PE=4 SV=1
MM1 pKa = 7.62TSTSTEE7 pKa = 3.88TLKK10 pKa = 11.4NKK12 pKa = 7.98GTAVKK17 pKa = 10.53GGIKK21 pKa = 9.95NAQPGDD27 pKa = 3.72LLFFDD32 pKa = 4.51TYY34 pKa = 10.87KK35 pKa = 11.08KK36 pKa = 10.13NGHH39 pKa = 4.46VVIYY43 pKa = 10.36LGNNQFIGAQGKK55 pKa = 7.34TGVGIVDD62 pKa = 3.64MNSSYY67 pKa = 11.42GKK69 pKa = 10.16YY70 pKa = 7.28FTKK73 pKa = 10.41RR74 pKa = 11.84FSGNIRR80 pKa = 11.84RR81 pKa = 11.84IAGGGNVGAALGGGGAAMSGAGTGTAYY108 pKa = 10.32RR109 pKa = 11.84QAPSNLMGPINNAAKK124 pKa = 10.29QYY126 pKa = 10.27GVNPNLIAAIIKK138 pKa = 10.22KK139 pKa = 10.2EE140 pKa = 4.13STFKK144 pKa = 11.0SGLTSSAGAKK154 pKa = 10.33GYY156 pKa = 7.86MQLMPATARR165 pKa = 11.84AMGVKK170 pKa = 10.22NPWDD174 pKa = 3.58TQQNINGGTKK184 pKa = 10.51YY185 pKa = 9.98IAQQLKK191 pKa = 8.97TYY193 pKa = 10.29KK194 pKa = 10.78NNIPLALAAYY204 pKa = 9.31NWGPGNLNKK213 pKa = 10.5AIRR216 pKa = 11.84KK217 pKa = 9.59AGGSKK222 pKa = 10.02DD223 pKa = 2.92WNQIRR228 pKa = 11.84RR229 pKa = 11.84FAPKK233 pKa = 8.44EE234 pKa = 3.76TRR236 pKa = 11.84DD237 pKa = 3.79YY238 pKa = 11.28VDD240 pKa = 5.67KK241 pKa = 11.37IMGWRR246 pKa = 3.39

Molecular weight: 26.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12721

30

1679

254.4

28.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.586 ± 0.356

0.723 ± 0.145

5.628 ± 0.253

7.083 ± 0.483

4.19 ± 0.184

6.91 ± 0.396

1.627 ± 0.146

5.864 ± 0.358

8.286 ± 0.509

7.193 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.877 ± 0.196

6.21 ± 0.267

3.608 ± 0.35

5.039 ± 0.553

4.221 ± 0.242

5.707 ± 0.276

6.037 ± 0.461

6.147 ± 0.22

1.187 ± 0.145

3.875 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski