Buttiauxella agrestis ATCC 33320

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Buttiauxella; Buttiauxella agrestis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085G449|A0A085G449_9ENTR Putative flavoprotein OS=Buttiauxella agrestis ATCC 33320 OX=1006004 GN=GBAG_3267 PE=4 SV=1
MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.12KK20 pKa = 10.0IRR22 pKa = 11.84DD23 pKa = 3.65IIGPEE28 pKa = 4.02LVTLHH33 pKa = 6.12NLKK36 pKa = 10.49DD37 pKa = 3.88DD38 pKa = 4.19SPAMMEE44 pKa = 4.15QYY46 pKa = 10.97DD47 pKa = 4.11VLILGIPTWDD57 pKa = 3.34FGEE60 pKa = 4.35LQEE63 pKa = 5.0DD64 pKa = 4.26WEE66 pKa = 4.59AVWDD70 pKa = 4.04QLDD73 pKa = 3.56NLNLEE78 pKa = 4.59GKK80 pKa = 9.18PVALYY85 pKa = 11.17GMGDD89 pKa = 3.29QLGYY93 pKa = 11.02GEE95 pKa = 4.87WFLDD99 pKa = 3.74ALGMLHH105 pKa = 7.51DD106 pKa = 4.42KK107 pKa = 10.32LAPRR111 pKa = 11.84GAQFIGYY118 pKa = 8.04WPTEE122 pKa = 4.1GYY124 pKa = 10.61EE125 pKa = 4.19FTSPKK130 pKa = 10.02PVIADD135 pKa = 3.41GQLFVGLALDD145 pKa = 3.64EE146 pKa = 4.38TNQYY150 pKa = 10.89DD151 pKa = 4.15LSDD154 pKa = 3.49EE155 pKa = 5.25RR156 pKa = 11.84IEE158 pKa = 4.1NWCDD162 pKa = 3.23QILIEE167 pKa = 4.27MAEE170 pKa = 4.11KK171 pKa = 10.15FAA173 pKa = 6.13

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085FZY7|A0A085FZY7_9ENTR Periplasmic alpha-amylase OS=Buttiauxella agrestis ATCC 33320 OX=1006004 GN=malS PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4332

0

4332

1401234

19

4216

323.5

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.51 ± 0.038

1.053 ± 0.015

5.171 ± 0.032

5.687 ± 0.04

3.942 ± 0.032

7.45 ± 0.04

2.27 ± 0.02

5.929 ± 0.031

4.331 ± 0.037

10.728 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.021

3.936 ± 0.029

4.434 ± 0.028

4.493 ± 0.033

5.336 ± 0.038

6.001 ± 0.029

5.45 ± 0.046

7.199 ± 0.032

1.485 ± 0.018

2.793 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski