Trichinella britovi (Parasitic roundworm)
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19931 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V1CIE4|A0A0V1CIE4_TRIBR Uncharacterized protein (Fragment) OS=Trichinella britovi OX=45882 GN=T03_4668 PE=3 SV=1
MM1 pKa = 7.83 AILGVTNSPNFDD13 pKa = 5.83 AILCWLFHH21 pKa = 6.09 HH22 pKa = 7.06 VLMEE26 pKa = 3.83 WTTAYY31 pKa = 9.26 EE32 pKa = 3.82 WSEE35 pKa = 3.98 LLIHH39 pKa = 7.07 CDD41 pKa = 2.78 ASLYY45 pKa = 10.97 FEE47 pKa = 4.81 VSHH50 pKa = 6.25 LQNDD54 pKa = 4.32 FLHH57 pKa = 6.56 SRR59 pKa = 11.84 IRR61 pKa = 11.84 PSGNLNAVDD70 pKa = 3.65 SANRR74 pKa = 11.84 PFFVGDD80 pKa = 3.82 EE81 pKa = 4.15 YY82 pKa = 11.62 HH83 pKa = 7.2 CIDD86 pKa = 4.06 PFFDD90 pKa = 3.0 ISAFVDD96 pKa = 3.67 EE97 pKa = 5.32 EE98 pKa = 4.33 KK99 pKa = 11.08 APSLDD104 pKa = 3.37 SSLDD108 pKa = 3.5 EE109 pKa = 4.44 SVLLGGWTSDD119 pKa = 3.78 DD120 pKa = 3.57 NSGEE124 pKa = 4.17 SSVSEE129 pKa = 3.71 ISSSISEE136 pKa = 4.06 YY137 pKa = 10.63 SLEE140 pKa = 3.91 GRR142 pKa = 11.84 ISSLVDD148 pKa = 3.65 DD149 pKa = 5.2 LSDD152 pKa = 3.25 MM153 pKa = 4.73
Molecular weight: 17.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.961
IPC_protein 3.935
Toseland 3.732
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.757
Grimsley 3.643
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.24
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.05
Patrickios 1.074
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.936
Protein with the highest isoelectric point:
>tr|A0A0V0YXX0|A0A0V0YXX0_TRIBR Uncharacterized protein (Fragment) OS=Trichinella britovi OX=45882 GN=T03_11587 PE=4 SV=1
MM1 pKa = 6.95 ITSKK5 pKa = 10.62 AARR8 pKa = 11.84 WMNTWRR14 pKa = 11.84 TKK16 pKa = 7.65 WKK18 pKa = 10.22 RR19 pKa = 11.84 GRR21 pKa = 11.84 FWHH24 pKa = 5.69 SAARR28 pKa = 11.84 KK29 pKa = 8.03 KK30 pKa = 10.45 QSPSRR35 pKa = 11.84 PFMMRR40 pKa = 11.84 KK41 pKa = 9.49 LPP43 pKa = 3.77
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.475
IPC2_protein 11.052
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.574
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.31
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15466
4465
19931
9386352
30
15218
470.9
53.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.449 ± 0.017
2.65 ± 0.018
5.252 ± 0.015
6.345 ± 0.021
4.555 ± 0.014
4.989 ± 0.018
2.457 ± 0.007
5.535 ± 0.014
5.985 ± 0.019
9.722 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.007
4.96 ± 0.012
4.366 ± 0.017
4.275 ± 0.016
5.677 ± 0.016
8.353 ± 0.02
5.299 ± 0.012
6.433 ± 0.012
1.212 ± 0.006
3.077 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here