Yokapox virus
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 184 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3EIB0|G3EIB0_9POXV Protein F15 OS=Yokapox virus OX=1076255 GN=YKV032c PE=3 SV=1
MM1 pKa = 7.59 EE2 pKa = 6.03 EE3 pKa = 3.47 IALYY7 pKa = 10.86 DD8 pKa = 3.84 EE9 pKa = 5.75 DD10 pKa = 6.11 SILLSSEE17 pKa = 3.96 LNNIISIIEE26 pKa = 4.27 NGDD29 pKa = 4.23 DD30 pKa = 3.67 IPNNIYY36 pKa = 10.75 NIITITSDD44 pKa = 3.09 AYY46 pKa = 11.91 NMIKK50 pKa = 10.54 NLTDD54 pKa = 3.27 NDD56 pKa = 3.85 INNIICFLNTLNDD69 pKa = 3.23 NYY71 pKa = 11.13 DD72 pKa = 3.35 IVKK75 pKa = 10.59 SSFDD79 pKa = 3.53 TLISKK84 pKa = 10.69 LNDD87 pKa = 2.93 ITHH90 pKa = 7.05 EE91 pKa = 4.22 DD92 pKa = 3.52 MNITFDD98 pKa = 4.87 DD99 pKa = 4.48 LLNMLFIIKK108 pKa = 10.18 NLTDD112 pKa = 3.46 EE113 pKa = 4.56 EE114 pKa = 4.74 FNNLLNNFVDD124 pKa = 6.41 DD125 pKa = 5.11 INNDD129 pKa = 3.49 DD130 pKa = 4.37 NIEE133 pKa = 4.26 LRR135 pKa = 11.84 TEE137 pKa = 4.26 LNNFLYY143 pKa = 10.16 SINMAIDD150 pKa = 5.82 DD151 pKa = 4.12 ITNDD155 pKa = 3.01 INMDD159 pKa = 4.24 TISILDD165 pKa = 4.17 IILDD169 pKa = 3.82 SLNSYY174 pKa = 9.04 IQQ176 pKa = 3.14
Molecular weight: 20.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.965
IPC2_protein 3.3
IPC_protein 3.325
Toseland 3.096
ProMoST 3.528
Dawson 3.338
Bjellqvist 3.503
Wikipedia 3.312
Rodwell 3.147
Grimsley 3.007
Solomon 3.312
Lehninger 3.274
Nozaki 3.465
DTASelect 3.732
Thurlkill 3.172
EMBOSS 3.325
Sillero 3.452
Patrickios 0.833
IPC_peptide 3.312
IPC2_peptide 3.414
IPC2.peptide.svr19 3.636
Protein with the highest isoelectric point:
>tr|G3EI10|G3EI10_9POXV Nonessential IMV membrane protein OS=Yokapox virus OX=1076255 GN=YKV118c PE=4 SV=1
MM1 pKa = 7.45 ILSIILIGVCIVIVGGIIYY20 pKa = 10.24 AVYY23 pKa = 10.13 YY24 pKa = 10.37 RR25 pKa = 11.84 NKK27 pKa = 9.78 QRR29 pKa = 11.84 PNNSNNNKK37 pKa = 6.9 KK38 pKa = 8.07 TKK40 pKa = 10.08 YY41 pKa = 9.75 VNSLGKK47 pKa = 9.39 EE48 pKa = 4.33 HH49 pKa = 7.56 IDD51 pKa = 3.54 SLYY54 pKa = 11.21 NLFKK58 pKa = 10.93 SSPP61 pKa = 3.44
Molecular weight: 6.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.162
IPC2_protein 9.458
IPC_protein 9.399
Toseland 9.78
ProMoST 9.589
Dawson 10.058
Bjellqvist 9.78
Wikipedia 10.262
Rodwell 10.409
Grimsley 10.16
Solomon 10.101
Lehninger 10.072
Nozaki 9.809
DTASelect 9.765
Thurlkill 9.882
EMBOSS 10.204
Sillero 9.984
Patrickios 7.468
IPC_peptide 10.101
IPC2_peptide 8.521
IPC2.peptide.svr19 8.337
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
184
0
184
53878
33
1852
292.8
33.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.613 ± 0.102
2.032 ± 0.099
6.502 ± 0.115
5.104 ± 0.134
4.8 ± 0.157
3.61 ± 0.117
1.867 ± 0.068
11.528 ± 0.189
8.428 ± 0.152
8.603 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.522 ± 0.077
8.504 ± 0.179
2.905 ± 0.109
1.901 ± 0.075
3.465 ± 0.108
7.814 ± 0.135
5.605 ± 0.135
5.551 ± 0.111
0.666 ± 0.046
5.978 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here