Hubei tombus-like virus 40

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG08|A0A1L3KG08_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 40 OX=1923289 PE=4 SV=1
MM1 pKa = 7.71PALTSDD7 pKa = 4.93LVTAPAKK14 pKa = 10.35SLQNMITGLEE24 pKa = 4.05TRR26 pKa = 11.84VLNLVPKK33 pKa = 9.41TSPLLAPSDD42 pKa = 3.76EE43 pKa = 4.18VAIIVVAVEE52 pKa = 4.35EE53 pKa = 4.39EE54 pKa = 4.37GHH56 pKa = 6.23PLSYY60 pKa = 11.01DD61 pKa = 2.98NHH63 pKa = 8.06LDD65 pKa = 3.52VLPCLGYY72 pKa = 10.4RR73 pKa = 11.84VIPMDD78 pKa = 3.86RR79 pKa = 11.84ALPPPRR85 pKa = 11.84PFEE88 pKa = 4.22TMVDD92 pKa = 4.04LQPVMDD98 pKa = 4.31RR99 pKa = 11.84YY100 pKa = 10.13EE101 pKa = 3.74RR102 pKa = 11.84QIRR105 pKa = 11.84YY106 pKa = 6.19NQKK109 pKa = 10.19RR110 pKa = 11.84GWKK113 pKa = 8.06TVILYY118 pKa = 10.63RR119 pKa = 11.84NGILLPGVYY128 pKa = 9.45PRR130 pKa = 11.84EE131 pKa = 4.13TGCIWRR137 pKa = 11.84LRR139 pKa = 11.84RR140 pKa = 11.84TIDD143 pKa = 3.32NGVEE147 pKa = 4.05CYY149 pKa = 7.78YY150 pKa = 8.9TTVARR155 pKa = 11.84SNSTIVRR162 pKa = 11.84HH163 pKa = 5.73PLINMQVEE171 pKa = 4.9PYY173 pKa = 8.99STEE176 pKa = 3.7LQPPVAGLHH185 pKa = 6.12GEE187 pKa = 4.13LSKK190 pKa = 10.71VTVTMPCRR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 10.62DD201 pKa = 3.72NPLKK205 pKa = 10.46AGARR209 pKa = 11.84EE210 pKa = 4.01FVRR213 pKa = 11.84TEE215 pKa = 3.51EE216 pKa = 4.19MAWLGDD222 pKa = 3.5VVAPVPFNEE231 pKa = 3.89WVKK234 pKa = 10.52RR235 pKa = 11.84YY236 pKa = 8.28PLKK239 pKa = 10.65RR240 pKa = 11.84RR241 pKa = 11.84EE242 pKa = 4.21QLSEE246 pKa = 3.61AHH248 pKa = 7.16DD249 pKa = 3.95RR250 pKa = 11.84LLQLGMLGTRR260 pKa = 11.84EE261 pKa = 4.24SIVKK265 pKa = 9.96NFLKK269 pKa = 10.4IEE271 pKa = 4.2TTTKK275 pKa = 10.75GVDD278 pKa = 3.45CRR280 pKa = 11.84NISPRR285 pKa = 11.84SDD287 pKa = 2.95VFLACVGPYY296 pKa = 9.32IAAVEE301 pKa = 4.25HH302 pKa = 6.45EE303 pKa = 4.51ALNAPFLVKK312 pKa = 10.25GRR314 pKa = 11.84NIRR317 pKa = 11.84ARR319 pKa = 11.84DD320 pKa = 3.1KK321 pKa = 11.13WMGSLSRR328 pKa = 11.84FSHH331 pKa = 7.18FIEE334 pKa = 5.07IDD336 pKa = 3.38FARR339 pKa = 11.84FDD341 pKa = 3.55MTLSKK346 pKa = 10.72EE347 pKa = 3.8LLEE350 pKa = 4.42NVEE353 pKa = 4.6HH354 pKa = 6.8EE355 pKa = 4.32LLLRR359 pKa = 11.84PFSEE363 pKa = 4.35DD364 pKa = 2.9QHH366 pKa = 8.33ALFHH370 pKa = 7.17IFMKK374 pKa = 10.15MALRR378 pKa = 11.84TKK380 pKa = 10.17GVSEE384 pKa = 4.78FGTSYY389 pKa = 10.51RR390 pKa = 11.84ISGTRR395 pKa = 11.84CSGDD399 pKa = 2.7AHH401 pKa = 5.81TSIGNGLLNRR411 pKa = 11.84FAIWWCLRR419 pKa = 11.84YY420 pKa = 9.63IPEE423 pKa = 3.93QDD425 pKa = 2.36WVSFHH430 pKa = 6.9EE431 pKa = 4.98GDD433 pKa = 3.99DD434 pKa = 3.99GVIGLVEE441 pKa = 4.03GRR443 pKa = 11.84VDD445 pKa = 3.42EE446 pKa = 4.79ARR448 pKa = 11.84AALEE452 pKa = 4.14SLWCLGLQAKK462 pKa = 9.73IDD464 pKa = 3.95VYY466 pKa = 11.28HH467 pKa = 7.32DD468 pKa = 4.07LSQTSFCGRR477 pKa = 11.84FMADD481 pKa = 2.95TASGMMSYY489 pKa = 10.72CDD491 pKa = 4.15PLRR494 pKa = 11.84ALAKK498 pKa = 10.15FHH500 pKa = 5.87TTVSDD505 pKa = 3.97GDD507 pKa = 3.97PKK509 pKa = 11.62ALMLAKK515 pKa = 10.31ALSYY519 pKa = 11.26YY520 pKa = 9.86STDD523 pKa = 2.96RR524 pKa = 11.84NTPVVGVLCYY534 pKa = 10.9ALITILRR541 pKa = 11.84PEE543 pKa = 4.06VTAKK547 pKa = 10.5RR548 pKa = 11.84LRR550 pKa = 11.84KK551 pKa = 9.4ALRR554 pKa = 11.84FNARR558 pKa = 11.84NLSWFAQYY566 pKa = 10.88EE567 pKa = 4.14DD568 pKa = 6.01AEE570 pKa = 5.09DD571 pKa = 3.88FWQYY575 pKa = 7.37WRR577 pKa = 11.84RR578 pKa = 11.84GVTRR582 pKa = 11.84PCCDD586 pKa = 2.68ILRR589 pKa = 11.84PFFALRR595 pKa = 11.84SGVSIRR601 pKa = 11.84EE602 pKa = 4.07QQSLEE607 pKa = 3.81QMFDD611 pKa = 2.55NWVSVGYY618 pKa = 10.73VPGEE622 pKa = 3.84FGKK625 pKa = 10.47IPVDD629 pKa = 3.26WTPLAEE635 pKa = 4.55HH636 pKa = 6.65KK637 pKa = 9.54WVTMRR642 pKa = 11.84TSDD645 pKa = 2.84WVTT648 pKa = 2.92

Molecular weight: 73.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFY2|A0A1L3KFY2_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 40 OX=1923289 PE=4 SV=1
MM1 pKa = 7.42LSYY4 pKa = 11.47YY5 pKa = 9.47EE6 pKa = 4.33GRR8 pKa = 11.84WIVLISVGVCIMVHH22 pKa = 6.32AEE24 pKa = 3.86YY25 pKa = 10.95VSAVWAVAGYY35 pKa = 8.03TVMGYY40 pKa = 8.12TYY42 pKa = 10.87SVARR46 pKa = 11.84TWGRR50 pKa = 11.84YY51 pKa = 5.24VVRR54 pKa = 11.84VRR56 pKa = 11.84SGTQFGRR63 pKa = 11.84WNEE66 pKa = 3.76IADD69 pKa = 3.68MFRR72 pKa = 11.84QYY74 pKa = 11.33VGTLDD79 pKa = 4.33RR80 pKa = 11.84PVHH83 pKa = 4.64QHH85 pKa = 4.02GHH87 pKa = 5.59HH88 pKa = 7.08NILAWEE94 pKa = 4.03RR95 pKa = 11.84RR96 pKa = 11.84VAEE99 pKa = 4.03EE100 pKa = 3.86FVVNVYY106 pKa = 10.77LGVQEE111 pKa = 5.04RR112 pKa = 11.84IRR114 pKa = 11.84DD115 pKa = 3.67VGGSRR120 pKa = 11.84TRR122 pKa = 11.84HH123 pKa = 5.51RR124 pKa = 11.84ALGDD128 pKa = 3.44RR129 pKa = 11.84KK130 pKa = 9.83HH131 pKa = 6.12VCGPTLQALDD141 pKa = 3.81NMRR144 pKa = 11.84EE145 pKa = 4.16DD146 pKa = 3.82KK147 pKa = 11.19DD148 pKa = 3.63RR149 pKa = 11.84DD150 pKa = 3.72AVFSNCRR157 pKa = 11.84KK158 pKa = 10.3SGGDD162 pKa = 3.36CPLRR166 pKa = 11.84EE167 pKa = 4.96LIPVAMLSHH176 pKa = 6.59TDD178 pKa = 3.58YY179 pKa = 11.36YY180 pKa = 9.8CTNEE184 pKa = 4.08EE185 pKa = 4.06LCSIVTGPTFIINHH199 pKa = 6.63DD200 pKa = 3.81FDD202 pKa = 4.02STTEE206 pKa = 3.93LGVYY210 pKa = 9.62EE211 pKa = 5.64DD212 pKa = 4.86EE213 pKa = 5.47DD214 pKa = 3.78EE215 pKa = 4.41QRR217 pKa = 11.84CEE219 pKa = 3.74ARR221 pKa = 11.84ATITGGQVTMQPEE234 pKa = 4.33AGSPYY239 pKa = 10.23GPHH242 pKa = 7.55RR243 pKa = 11.84YY244 pKa = 9.08HH245 pKa = 6.46LWKK248 pKa = 10.68SEE250 pKa = 3.8GVVVSRR256 pKa = 11.84TGAFTYY262 pKa = 10.38RR263 pKa = 11.84KK264 pKa = 9.48LGTIGSTTVLLGYY277 pKa = 8.41PCRR280 pKa = 11.84GIYY283 pKa = 10.23RR284 pKa = 11.84SDD286 pKa = 4.43DD287 pKa = 3.48SCQLAFADD295 pKa = 3.7QRR297 pKa = 11.84AGARR301 pKa = 11.84LSEE304 pKa = 4.29RR305 pKa = 11.84GDD307 pKa = 3.53VYY309 pKa = 11.42GKK311 pKa = 10.87GGMYY315 pKa = 10.46YY316 pKa = 10.59VDD318 pKa = 3.97GEE320 pKa = 4.42NIIFTEE326 pKa = 4.25TSDD329 pKa = 3.73RR330 pKa = 11.84LGPTGDD336 pKa = 4.07ADD338 pKa = 4.3CLPLAVLEE346 pKa = 4.64GVALQLATANRR357 pKa = 11.84DD358 pKa = 3.41EE359 pKa = 5.26KK360 pKa = 11.16YY361 pKa = 10.29LSHH364 pKa = 7.25LISYY368 pKa = 7.55TTAKK372 pKa = 10.4LHH374 pKa = 5.33AQRR377 pKa = 11.84IRR379 pKa = 11.84FRR381 pKa = 11.84RR382 pKa = 11.84GEE384 pKa = 4.23AIARR388 pKa = 11.84WVAKK392 pKa = 10.3RR393 pKa = 11.84ADD395 pKa = 3.92EE396 pKa = 4.25LALGLVQNCSLPIGDD411 pKa = 5.7PIHH414 pKa = 6.9ISTFGVIYY422 pKa = 10.0YY423 pKa = 9.95RR424 pKa = 11.84VIRR427 pKa = 11.84WVLDD431 pKa = 3.3RR432 pKa = 11.84LNKK435 pKa = 10.15FNVAEE440 pKa = 4.05YY441 pKa = 10.72LHH443 pKa = 6.39EE444 pKa = 4.45CLYY447 pKa = 11.38KK448 pKa = 10.78NLSTHH453 pKa = 5.91YY454 pKa = 9.82ALPHH458 pKa = 4.93MWVARR463 pKa = 11.84EE464 pKa = 3.72IPAYY468 pKa = 9.84NCEE471 pKa = 3.49VDD473 pKa = 4.26AIRR476 pKa = 11.84VRR478 pKa = 11.84LGRR481 pKa = 11.84LPRR484 pKa = 11.84VNRR487 pKa = 11.84PFRR490 pKa = 11.84HH491 pKa = 5.75EE492 pKa = 4.28GPRR495 pKa = 11.84ADD497 pKa = 3.28ARR499 pKa = 11.84ANIGPGHH506 pKa = 7.28RR507 pKa = 11.84PRR509 pKa = 11.84QEE511 pKa = 3.75PAEE514 pKa = 4.36HH515 pKa = 7.54DD516 pKa = 3.29NGARR520 pKa = 11.84NARR523 pKa = 11.84PEE525 pKa = 4.32FGAQNQPPAGAFGRR539 pKa = 11.84GGNYY543 pKa = 9.52RR544 pKa = 11.84GRR546 pKa = 11.84GRR548 pKa = 11.84GRR550 pKa = 11.84GAPAVVRR557 pKa = 11.84QPFRR561 pKa = 11.84RR562 pKa = 11.84PALPRR567 pKa = 11.84IPRR570 pKa = 11.84NPNGPRR576 pKa = 11.84VAPAEE581 pKa = 3.98AVRR584 pKa = 11.84DD585 pKa = 4.18DD586 pKa = 4.39GGPAAGDD593 pKa = 3.77GQIRR597 pKa = 11.84AADD600 pKa = 3.49PVQPEE605 pKa = 4.24EE606 pKa = 4.17GVEE609 pKa = 4.26DD610 pKa = 4.38GDD612 pKa = 5.97IVPEE616 pKa = 3.91WDD618 pKa = 3.35LVAWSIPEE626 pKa = 3.75RR627 pKa = 11.84DD628 pKa = 3.37RR629 pKa = 11.84LYY631 pKa = 11.44LEE633 pKa = 3.94VAEE636 pKa = 5.3NDD638 pKa = 3.5RR639 pKa = 11.84QRR641 pKa = 11.84CRR643 pKa = 11.84VLLHH647 pKa = 6.08NRR649 pKa = 11.84RR650 pKa = 11.84QVEE653 pKa = 4.72FYY655 pKa = 11.23NRR657 pKa = 11.84AAPFDD662 pKa = 3.72QHH664 pKa = 7.18AGRR667 pKa = 11.84ALLHH671 pKa = 6.28RR672 pKa = 11.84VATTRR677 pKa = 11.84GRR679 pKa = 11.84ITRR682 pKa = 11.84RR683 pKa = 11.84TIEE686 pKa = 4.74GYY688 pKa = 10.91CNDD691 pKa = 3.56ALQEE695 pKa = 4.02EE696 pKa = 4.68RR697 pKa = 11.84QPAQGRR703 pKa = 11.84GQGVRR708 pKa = 11.84PHH710 pKa = 6.9RR711 pKa = 11.84GDD713 pKa = 3.21GVVRR717 pKa = 11.84GRR719 pKa = 11.84GRR721 pKa = 11.84ARR723 pKa = 11.84PFQRR727 pKa = 11.84VGG729 pKa = 2.94

Molecular weight: 81.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1857

480

729

619.0

69.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.774

2.423 ± 0.301

4.685 ± 0.787

5.924 ± 0.294

2.854 ± 0.475

7.593 ± 1.219

2.423 ± 0.492

4.631 ± 0.144

3.016 ± 0.764

9.532 ± 1.506

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.652

3.069 ± 0.345

5.439 ± 0.272

3.285 ± 0.273

9.155 ± 1.726

6.354 ± 1.871

6.139 ± 1.032

7.324 ± 0.708

2.208 ± 0.366

3.931 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski