Beihai weivirus-like virus 16
Average proteome isoelectric point is 7.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLA5|A0A1L3KLA5_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 16 OX=1922744 PE=4 SV=1
MM1 pKa = 7.48 HH2 pKa = 7.13 SFVPRR7 pKa = 11.84 PEE9 pKa = 4.55 CYY11 pKa = 10.24 APLCSRR17 pKa = 11.84 CDD19 pKa = 3.63 DD20 pKa = 3.32 QSFRR24 pKa = 11.84 SLQTGRR30 pKa = 11.84 VEE32 pKa = 3.4 IHH34 pKa = 6.11 MLVTDD39 pKa = 4.0 GCEE42 pKa = 3.88 ACRR45 pKa = 11.84 SLIPSCLRR53 pKa = 11.84 EE54 pKa = 3.89 FTRR57 pKa = 11.84 TVRR60 pKa = 11.84 TFRR63 pKa = 11.84 LEE65 pKa = 3.93 DD66 pKa = 3.45 DD67 pKa = 4.37 FFARR71 pKa = 11.84 LCIDD75 pKa = 3.4 YY76 pKa = 8.92 STAADD81 pKa = 3.36 KK82 pKa = 11.16 SLNQSLTSEE91 pKa = 4.06 IRR93 pKa = 11.84 SEE95 pKa = 3.95 KK96 pKa = 10.76 DD97 pKa = 2.83 PLRR100 pKa = 11.84 RR101 pKa = 11.84 VAMMRR106 pKa = 11.84 LVPEE110 pKa = 4.62 ALSQVHH116 pKa = 5.98 ARR118 pKa = 11.84 QRR120 pKa = 11.84 LLSPRR125 pKa = 11.84 FNRR128 pKa = 11.84 HH129 pKa = 4.46 SLFLYY134 pKa = 10.29 GAGGLTTVTVLTLGTRR150 pKa = 11.84 RR151 pKa = 11.84 LLANWDD157 pKa = 3.3 MPKK160 pKa = 10.0 PVRR163 pKa = 11.84 YY164 pKa = 9.7 GLTAFTMFPFWEE176 pKa = 4.12 MGVFFLGRR184 pKa = 11.84 KK185 pKa = 8.47 VSQMWSGHH193 pKa = 4.23 AHH195 pKa = 5.52 YY196 pKa = 10.31 KK197 pKa = 9.93 ILPLRR202 pKa = 11.84 DD203 pKa = 3.08 ITEE206 pKa = 4.35 TEE208 pKa = 4.3 DD209 pKa = 3.35 EE210 pKa = 5.44 DD211 pKa = 4.37 IVEE214 pKa = 4.8 DD215 pKa = 3.96 PAVPAPVPRR224 pKa = 11.84 IAPTLPEE231 pKa = 5.05 PDD233 pKa = 4.17 SDD235 pKa = 4.14 HH236 pKa = 7.27 SDD238 pKa = 3.44 DD239 pKa = 3.98 VSSVTTDD246 pKa = 3.52 PGAHH250 pKa = 6.08 MDD252 pKa = 4.57 EE253 pKa = 4.05 IRR255 pKa = 11.84 NWFQNLPPPPGLGPDD270 pKa = 3.56 DD271 pKa = 5.52 RR272 pKa = 11.84 IPVNTIVGPLEE283 pKa = 4.18 SVILEE288 pKa = 4.21 TNIEE292 pKa = 4.11 SGEE295 pKa = 4.25 DD296 pKa = 3.21 YY297 pKa = 10.62 LARR300 pKa = 11.84 VEE302 pKa = 4.28 TRR304 pKa = 11.84 DD305 pKa = 3.28 IPVPRR310 pKa = 11.84 VATPIGPTTQEE321 pKa = 3.77 VQHH324 pKa = 6.32 YY325 pKa = 10.47 SDD327 pKa = 3.79 ALEE330 pKa = 4.39 NIIRR334 pKa = 11.84 ACKK337 pKa = 10.17 GRR339 pKa = 11.84 VEE341 pKa = 4.97 GKK343 pKa = 10.15 AKK345 pKa = 10.19 PFKK348 pKa = 10.62 PSDD351 pKa = 3.32 KK352 pKa = 10.27 VYY354 pKa = 11.15 AGILSVQRR362 pKa = 11.84 ALKK365 pKa = 10.53 DD366 pKa = 3.23 HH367 pKa = 6.48 VFTKK371 pKa = 10.48 RR372 pKa = 11.84 RR373 pKa = 11.84 VQQWAADD380 pKa = 3.91 NPCLNEE386 pKa = 4.04 LVSKK390 pKa = 10.13 KK391 pKa = 8.21 WSQRR395 pKa = 11.84 TVVNALDD402 pKa = 3.54 MLMATSANQHH412 pKa = 4.44 FVKK415 pKa = 10.0 WAVSIKK421 pKa = 10.92 DD422 pKa = 3.61 EE423 pKa = 3.92 ILAAGKK429 pKa = 8.79 EE430 pKa = 3.96 PRR432 pKa = 11.84 MIMSCGGAGQLCALLTVKK450 pKa = 10.41 CFEE453 pKa = 5.05 DD454 pKa = 4.72 LYY456 pKa = 10.97 FDD458 pKa = 3.45 HH459 pKa = 7.57 FEE461 pKa = 3.96 RR462 pKa = 11.84 RR463 pKa = 11.84 SIKK466 pKa = 10.03 HH467 pKa = 5.27 RR468 pKa = 11.84 PKK470 pKa = 8.75 MVAMRR475 pKa = 11.84 EE476 pKa = 4.13 VAEE479 pKa = 4.1 KK480 pKa = 9.63 MRR482 pKa = 11.84 YY483 pKa = 7.68 GHH485 pKa = 6.66 VYY487 pKa = 10.46 EE488 pKa = 4.98 NDD490 pKa = 2.96 GSAWDD495 pKa = 3.79 VTVSPEE501 pKa = 4.36 LRR503 pKa = 11.84 HH504 pKa = 5.35 TLEE507 pKa = 4.85 DD508 pKa = 5.13 PILEE512 pKa = 4.71 HH513 pKa = 7.38 IAMLLFSHH521 pKa = 6.72 GNFEE525 pKa = 5.82 LITWDD530 pKa = 3.37 MQGADD535 pKa = 3.59 LEE537 pKa = 4.44 DD538 pKa = 3.46 RR539 pKa = 11.84 RR540 pKa = 11.84 KK541 pKa = 10.62 DD542 pKa = 3.39 QLKK545 pKa = 10.8 ASFNKK550 pKa = 10.11 HH551 pKa = 4.51 GARR554 pKa = 11.84 ACIIVRR560 pKa = 11.84 AFRR563 pKa = 11.84 KK564 pKa = 10.3 SGDD567 pKa = 3.44 RR568 pKa = 11.84 GTSALNNLVNLTLWSAMVLDD588 pKa = 5.28 DD589 pKa = 4.28 PAQVVYY595 pKa = 10.48 KK596 pKa = 9.95 PSARR600 pKa = 11.84 KK601 pKa = 9.54 YY602 pKa = 10.44 RR603 pKa = 11.84 INGQNTDD610 pKa = 3.36 FAFFYY615 pKa = 10.48 EE616 pKa = 4.74 GDD618 pKa = 3.91 DD619 pKa = 4.31 SIVSCGQRR627 pKa = 11.84 LDD629 pKa = 3.6 VDD631 pKa = 3.87 HH632 pKa = 6.86 LTKK635 pKa = 10.11 EE636 pKa = 3.98 WEE638 pKa = 4.2 RR639 pKa = 11.84 AGMRR643 pKa = 11.84 PKK645 pKa = 10.6 LFHH648 pKa = 6.82 RR649 pKa = 11.84 KK650 pKa = 9.66 KK651 pKa = 9.62 GDD653 pKa = 2.47 AWTFCGVNGIAGTLAEE669 pKa = 5.03 PVPQLARR676 pKa = 11.84 NIASSAYY683 pKa = 10.0 SVSQGLDD690 pKa = 2.84 TGLARR695 pKa = 11.84 AMTLVGRR702 pKa = 11.84 VEE704 pKa = 4.25 NFRR707 pKa = 11.84 DD708 pKa = 3.54 SCPVFAHH715 pKa = 5.95 YY716 pKa = 8.0 FACIARR722 pKa = 11.84 HH723 pKa = 5.53 HH724 pKa = 6.35 LQGCTLKK731 pKa = 10.58 SVPMDD736 pKa = 3.95 RR737 pKa = 11.84 EE738 pKa = 4.09 SQMQIYY744 pKa = 10.19 GDD746 pKa = 3.88 YY747 pKa = 10.88 DD748 pKa = 3.67 DD749 pKa = 5.54 SRR751 pKa = 11.84 TVNIEE756 pKa = 3.78 DD757 pKa = 4.67 LLVVDD762 pKa = 5.11 NRR764 pKa = 11.84 TSTKK768 pKa = 9.25 GTDD771 pKa = 3.6 VIQAACGAPLTQEE784 pKa = 4.21 LVSAIAGLDD793 pKa = 3.37 TLGPEE798 pKa = 4.13 DD799 pKa = 3.29 TWIFRR804 pKa = 11.84 HH805 pKa = 6.02 MPAEE809 pKa = 4.19 WFHH812 pKa = 7.48 VPAA815 pKa = 5.9
Molecular weight: 91.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.036
IPC2_protein 5.957
IPC_protein 6.033
Toseland 6.364
ProMoST 6.44
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.491
Solomon 6.364
Lehninger 6.351
Nozaki 6.649
DTASelect 6.825
Thurlkill 6.839
EMBOSS 6.81
Sillero 6.751
Patrickios 4.329
IPC_peptide 6.376
IPC2_peptide 6.707
IPC2.peptide.svr19 6.639
Protein with the highest isoelectric point:
>tr|A0A1L3KLA5|A0A1L3KLA5_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 16 OX=1922744 PE=4 SV=1
MM1 pKa = 7.48 AQRR4 pKa = 11.84 KK5 pKa = 7.12 SRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 NGNSQQNGQQNGQQLALRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 NGRR33 pKa = 11.84 RR34 pKa = 11.84 GPNARR39 pKa = 11.84 TLVPGVGSTPGSPFGGIVGSDD60 pKa = 3.35 LRR62 pKa = 11.84 CWDD65 pKa = 3.46 AKK67 pKa = 11.05 LPLHH71 pKa = 6.59 LPLPRR76 pKa = 11.84 AVGPYY81 pKa = 9.14 TVIRR85 pKa = 11.84 CTRR88 pKa = 11.84 RR89 pKa = 11.84 INTANRR95 pKa = 11.84 QMLFGTFQDD104 pKa = 4.08 DD105 pKa = 3.99 DD106 pKa = 4.08 GTLGLVWNNICALGAINHH124 pKa = 6.83 HH125 pKa = 6.85 DD126 pKa = 4.91 PINAANNTQRR136 pKa = 11.84 YY137 pKa = 6.91 TIDD140 pKa = 3.28 LGGLGSGCSLVPAAMTVQVMNPNALQTTSGVIYY173 pKa = 10.15 AGVMNTQAAFGGDD186 pKa = 3.03 STAYY190 pKa = 10.26 GDD192 pKa = 3.93 RR193 pKa = 11.84 FDD195 pKa = 4.76 TFVEE199 pKa = 4.71 FQNPRR204 pKa = 11.84 LCAAPKK210 pKa = 9.52 LALRR214 pKa = 11.84 GVQMSSYY221 pKa = 8.83 PLNMSKK227 pKa = 10.41 VSEE230 pKa = 4.17 FTGLGVATDD239 pKa = 4.75 SVITWSDD246 pKa = 3.04 PTLRR250 pKa = 11.84 PTGWAPIMIYY260 pKa = 8.89 NTAGTTLEE268 pKa = 3.94 YY269 pKa = 10.61 LVTIEE274 pKa = 3.64 YY275 pKa = 10.1 RR276 pKa = 11.84 VRR278 pKa = 11.84 FDD280 pKa = 3.99 LDD282 pKa = 3.22 NVAVASHH289 pKa = 5.21 THH291 pKa = 5.52 YY292 pKa = 10.63 PVAKK296 pKa = 9.08 DD297 pKa = 3.52 TTWDD301 pKa = 3.69 KK302 pKa = 9.29 LTHH305 pKa = 5.48 MASMLGNGVQDD316 pKa = 3.07 IADD319 pKa = 3.41 IVSTVGGAARR329 pKa = 11.84 AMRR332 pKa = 11.84 PYY334 pKa = 9.98 ISSGSSLPMIAAA346 pKa = 4.56
Molecular weight: 37.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.926
IPC2_protein 9.092
IPC_protein 9.355
Toseland 9.37
ProMoST 9.399
Dawson 9.765
Bjellqvist 9.633
Wikipedia 10.014
Rodwell 9.809
Grimsley 9.867
Solomon 9.823
Lehninger 9.765
Nozaki 9.633
DTASelect 9.575
Thurlkill 9.589
EMBOSS 9.853
Sillero 9.75
Patrickios 4.762
IPC_peptide 9.809
IPC2_peptide 8.829
IPC2.peptide.svr19 7.867
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1161
346
815
580.5
64.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.527 ± 0.502
2.067 ± 0.309
6.546 ± 0.668
4.307 ± 1.566
3.704 ± 0.548
7.063 ± 1.517
2.67 ± 0.465
4.565 ± 0.029
3.359 ± 0.808
8.613 ± 0.259
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.101 ± 0.183
4.048 ± 1.148
5.857 ± 0.038
3.704 ± 0.457
7.666 ± 0.075
6.288 ± 0.035
6.632 ± 0.869
7.235 ± 0.148
1.637 ± 0.095
2.412 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here