Ageratum yellow vein China betasatellite
Average proteome isoelectric point is 4.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4VZ15|Q4VZ15_9VIRU C1 protein OS=Ageratum yellow vein China betasatellite OX=329288 GN=c1 PE=4 SV=1
MM1 pKa = 7.36 TISYY5 pKa = 9.77 TNDD8 pKa = 2.67 KK9 pKa = 11.11 GMLFIINVKK18 pKa = 9.53 FRR20 pKa = 11.84 SEE22 pKa = 3.45 IKK24 pKa = 10.2 FRR26 pKa = 11.84 VDD28 pKa = 3.07 VEE30 pKa = 4.47 VFSTKK35 pKa = 10.37 SPALVKK41 pKa = 10.77 NKK43 pKa = 9.63 FSIPYY48 pKa = 7.92 GHH50 pKa = 6.72 NGIIPPFNFNALEE63 pKa = 3.99 EE64 pKa = 4.84 GIKK67 pKa = 10.57 NILRR71 pKa = 11.84 IMYY74 pKa = 9.36 RR75 pKa = 11.84 DD76 pKa = 3.43 STIEE80 pKa = 3.91 EE81 pKa = 4.39 FKK83 pKa = 11.11 SEE85 pKa = 4.11 DD86 pKa = 3.35 MVEE89 pKa = 4.58 IIDD92 pKa = 4.9 IMMQEE97 pKa = 4.37 EE98 pKa = 4.71 APVIDD103 pKa = 3.22 IQVNDD108 pKa = 4.07 EE109 pKa = 3.96 YY110 pKa = 11.19 HH111 pKa = 5.85 VYY113 pKa = 11.12 KK114 pKa = 10.85 NVTVV118 pKa = 3.5
Molecular weight: 13.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.984
IPC2_protein 5.041
IPC_protein 4.902
Toseland 4.8
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.711
Rodwell 4.762
Grimsley 4.711
Solomon 4.851
Lehninger 4.8
Nozaki 4.965
DTASelect 5.092
Thurlkill 4.787
EMBOSS 4.736
Sillero 5.041
Patrickios 4.151
IPC_peptide 4.863
IPC2_peptide 5.029
IPC2.peptide.svr19 5.063
Protein with the highest isoelectric point:
>tr|Q4VZ15|Q4VZ15_9VIRU C1 protein OS=Ageratum yellow vein China betasatellite OX=329288 GN=c1 PE=4 SV=1
MM1 pKa = 7.36 TISYY5 pKa = 9.77 TNDD8 pKa = 2.67 KK9 pKa = 11.11 GMLFIINVKK18 pKa = 9.53 FRR20 pKa = 11.84 SEE22 pKa = 3.45 IKK24 pKa = 10.2 FRR26 pKa = 11.84 VDD28 pKa = 3.07 VEE30 pKa = 4.47 VFSTKK35 pKa = 10.37 SPALVKK41 pKa = 10.77 NKK43 pKa = 9.63 FSIPYY48 pKa = 7.92 GHH50 pKa = 6.72 NGIIPPFNFNALEE63 pKa = 3.99 EE64 pKa = 4.84 GIKK67 pKa = 10.57 NILRR71 pKa = 11.84 IMYY74 pKa = 9.36 RR75 pKa = 11.84 DD76 pKa = 3.43 STIEE80 pKa = 3.91 EE81 pKa = 4.39 FKK83 pKa = 11.11 SEE85 pKa = 4.11 DD86 pKa = 3.35 MVEE89 pKa = 4.58 IIDD92 pKa = 4.9 IMMQEE97 pKa = 4.37 EE98 pKa = 4.71 APVIDD103 pKa = 3.22 IQVNDD108 pKa = 4.07 EE109 pKa = 3.96 YY110 pKa = 11.19 HH111 pKa = 5.85 VYY113 pKa = 11.12 KK114 pKa = 10.85 NVTVV118 pKa = 3.5
Molecular weight: 13.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.984
IPC2_protein 5.041
IPC_protein 4.902
Toseland 4.8
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.711
Rodwell 4.762
Grimsley 4.711
Solomon 4.851
Lehninger 4.8
Nozaki 4.965
DTASelect 5.092
Thurlkill 4.787
EMBOSS 4.736
Sillero 5.041
Patrickios 4.151
IPC_peptide 4.863
IPC2_peptide 5.029
IPC2.peptide.svr19 5.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.542 ± 0.0
0.0 ± 0.0
5.932 ± 0.0
9.322 ± 0.0
6.78 ± 0.0
3.39 ± 0.0
1.695 ± 0.0
13.559 ± 0.0
7.627 ± 0.0
3.39 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.085 ± 0.0
7.627 ± 0.0
4.237 ± 0.0
1.695 ± 0.0
3.39 ± 0.0
5.932 ± 0.0
4.237 ± 0.0
9.322 ± 0.0
0.0 ± 0.0
4.237 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here