Mesorhizobium soli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7S029|A0A2P7S029_9RHIZ Uncharacterized protein OS=Mesorhizobium soli OX=1295366 GN=C7I85_26320 PE=4 SV=1
MM1 pKa = 7.49IPALSAQMMNGLGGGDD17 pKa = 3.95PGWIPEE23 pKa = 4.21GAVAHH28 pKa = 6.35LNFIGGNYY36 pKa = 8.8FAGGQSRR43 pKa = 11.84TVDD46 pKa = 3.89TILGGAFLPVSIDD59 pKa = 3.3PSMGMYY65 pKa = 10.33VDD67 pKa = 3.45NTSPNRR73 pKa = 11.84PNAIGPLFDD82 pKa = 6.7DD83 pKa = 4.32IAAGLAAGCTVVFEE97 pKa = 4.98IQFQDD102 pKa = 3.29DD103 pKa = 4.43PYY105 pKa = 11.64GSFIHH110 pKa = 7.16LMDD113 pKa = 4.58AAGANAATEE122 pKa = 4.49WVSGTIFGGAAYY134 pKa = 10.35VQDD137 pKa = 3.87QEE139 pKa = 5.7DD140 pKa = 3.98IEE142 pKa = 4.5LSAGAGSLTTAGLHH156 pKa = 6.38KK157 pKa = 10.28IAFTFARR164 pKa = 11.84RR165 pKa = 11.84ADD167 pKa = 3.47GDD169 pKa = 3.5FEE171 pKa = 4.74YY172 pKa = 10.72AVSSDD177 pKa = 3.1GSVAEE182 pKa = 4.32TMTVDD187 pKa = 3.67YY188 pKa = 11.22APLSPVNTITLFHH201 pKa = 7.36DD202 pKa = 4.37GDD204 pKa = 4.02DD205 pKa = 3.9TSILDD210 pKa = 4.06DD211 pKa = 3.69AWVHH215 pKa = 7.07SITLYY220 pKa = 10.19PAVAPAGLPALTAA233 pKa = 4.25

Molecular weight: 24.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7S4K0|A0A2P7S4K0_9RHIZ Uncharacterized protein OS=Mesorhizobium soli OX=1295366 GN=C7I85_22755 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.15GGRR28 pKa = 11.84AVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5837

0

5837

1783834

29

3998

305.6

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.105 ± 0.043

0.805 ± 0.01

5.532 ± 0.022

5.797 ± 0.032

3.903 ± 0.022

8.52 ± 0.036

2.02 ± 0.016

5.477 ± 0.023

3.65 ± 0.028

9.933 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.014

2.784 ± 0.021

5.001 ± 0.023

3.06 ± 0.018

6.753 ± 0.034

5.616 ± 0.025

5.279 ± 0.029

7.533 ± 0.027

1.347 ± 0.014

2.311 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski