Arthrobacter phage Dewayne

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Decurrovirus; unclassified Decurrovirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345M4H3|A0A345M4H3_9CAUD Major tail protein OS=Arthrobacter phage Dewayne OX=2283241 GN=10 PE=4 SV=1
MM1 pKa = 7.29RR2 pKa = 11.84TMGNSLKK9 pKa = 10.69DD10 pKa = 3.21IADD13 pKa = 3.66MVTAAGVPAAVDD25 pKa = 3.62PRR27 pKa = 11.84DD28 pKa = 3.77LNLPGAWVTPGLVSFDD44 pKa = 3.65VLDD47 pKa = 3.6VDD49 pKa = 4.07TAFMTFDD56 pKa = 3.68IYY58 pKa = 11.28LVAPDD63 pKa = 4.23HH64 pKa = 6.37GAVHH68 pKa = 6.69SLNSLGDD75 pKa = 3.72MLAKK79 pKa = 10.02IRR81 pKa = 11.84PALQVGEE88 pKa = 4.45AMPVMVNLPNHH99 pKa = 6.38GADD102 pKa = 4.12ALPALLISIDD112 pKa = 3.6AQLTEE117 pKa = 4.5DD118 pKa = 3.42

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345M4H5|A0A345M4H5_9CAUD Uncharacterized protein OS=Arthrobacter phage Dewayne OX=2283241 GN=12 PE=4 SV=1
MM1 pKa = 6.88GTKK4 pKa = 10.26LYY6 pKa = 10.93AVVGGAKK13 pKa = 9.71LRR15 pKa = 11.84STLRR19 pKa = 11.84KK20 pKa = 9.66AGADD24 pKa = 3.43MKK26 pKa = 10.74EE27 pKa = 3.83LSAVNRR33 pKa = 11.84DD34 pKa = 3.12VANIVLPVARR44 pKa = 11.84ATAPTTSGKK53 pKa = 10.47LGSTVRR59 pKa = 11.84AGATQKK65 pKa = 10.84SAIIRR70 pKa = 11.84VGSAKK75 pKa = 10.36APYY78 pKa = 9.74GPVVHH83 pKa = 6.22YY84 pKa = 7.52WHH86 pKa = 7.23KK87 pKa = 11.36GNYY90 pKa = 7.62TPNPWVSLAAQKK102 pKa = 9.29TEE104 pKa = 4.13PTWLARR110 pKa = 11.84YY111 pKa = 8.27HH112 pKa = 6.75AGIEE116 pKa = 4.11RR117 pKa = 11.84IINQVTGAA125 pKa = 3.91

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

5024

67

665

193.2

20.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.893 ± 1.001

0.836 ± 0.266

5.752 ± 0.295

4.737 ± 0.492

2.727 ± 0.325

8.439 ± 0.501

1.652 ± 0.297

4.578 ± 0.328

3.682 ± 0.29

7.962 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.35

3.643 ± 0.454

5.414 ± 0.608

3.762 ± 0.266

5.553 ± 0.557

5.593 ± 0.27

6.708 ± 0.38

8.101 ± 0.451

1.871 ± 0.165

2.528 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski