Streptococcus phage Javan457

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AGQ1|A0A4D6AGQ1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan457 OX=2548180 GN=Javan457_0037 PE=4 SV=1
MM1 pKa = 7.94IYY3 pKa = 10.55LQGEE7 pKa = 4.67GIKK10 pKa = 10.45PEE12 pKa = 4.46DD13 pKa = 3.51MTIDD17 pKa = 3.5MSGVQALQSTIDD29 pKa = 3.67YY30 pKa = 10.88INFLEE35 pKa = 4.48SEE37 pKa = 4.16KK38 pKa = 10.86HH39 pKa = 4.62RR40 pKa = 4.24

Molecular weight: 4.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AE09|A0A4D6AE09_9CAUD Paratox OS=Streptococcus phage Javan457 OX=2548180 GN=Javan457_0001 PE=4 SV=1
MM1 pKa = 6.64NTKK4 pKa = 10.0EE5 pKa = 3.95MTSIEE10 pKa = 4.29IKK12 pKa = 10.45VLNAIKK18 pKa = 10.65NGASFDD24 pKa = 4.02LPIQARR30 pKa = 11.84EE31 pKa = 3.85LRR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 2.98IGLSKK40 pKa = 10.7RR41 pKa = 11.84KK42 pKa = 9.96LEE44 pKa = 4.0EE45 pKa = 3.9VIEE48 pKa = 4.17NLRR51 pKa = 11.84VTHH54 pKa = 6.42RR55 pKa = 11.84QPIVAKK61 pKa = 8.28KK62 pKa = 8.01TKK64 pKa = 9.86PNGYY68 pKa = 8.92YY69 pKa = 10.33LPKK72 pKa = 10.15TKK74 pKa = 10.31EE75 pKa = 3.76EE76 pKa = 4.08RR77 pKa = 11.84DD78 pKa = 3.49AGIAPYY84 pKa = 9.86RR85 pKa = 11.84AQIRR89 pKa = 11.84TEE91 pKa = 4.09QKK93 pKa = 9.77NLAAVMSINLEE104 pKa = 4.25DD105 pKa = 3.76YY106 pKa = 7.69WTKK109 pKa = 10.28QKK111 pKa = 11.06KK112 pKa = 9.56PDD114 pKa = 3.32GNQALKK120 pKa = 10.88

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

9519

40

911

216.3

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.322 ± 0.526

0.546 ± 0.099

6.66 ± 0.289

7.259 ± 0.387

3.698 ± 0.21

5.914 ± 0.43

1.607 ± 0.205

7.039 ± 0.244

8.824 ± 0.376

8.583 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.16

5.557 ± 0.23

2.5 ± 0.19

3.939 ± 0.192

4.16 ± 0.362

6.618 ± 0.372

6.618 ± 0.347

5.946 ± 0.249

1.156 ± 0.148

3.614 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski