White sturgeon adenovirus 1
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8PIX1|A0A4P8PIX1_9ADEN PVI OS=White sturgeon adenovirus 1 OX=2580388 PE=4 SV=1
MM1 pKa = 7.76 LLEE4 pKa = 3.89 FLEE7 pKa = 4.75 AFVEE11 pKa = 4.97 AIGSFLAYY19 pKa = 9.64 ICFCEE24 pKa = 4.25 EE25 pKa = 3.71 ASLLCIEE32 pKa = 4.46 VSEE35 pKa = 4.25 EE36 pKa = 3.69 QFEE39 pKa = 4.55 NFVKK43 pKa = 10.66 FCVEE47 pKa = 3.78 QTLLTLFITLFYY59 pKa = 10.52 ILRR62 pKa = 11.84 DD63 pKa = 3.31 GWW65 pKa = 3.6
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.981
IPC2_protein 3.986
IPC_protein 3.719
Toseland 3.617
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.541
Rodwell 3.592
Grimsley 3.554
Solomon 3.617
Lehninger 3.567
Nozaki 3.821
DTASelect 3.795
Thurlkill 3.656
EMBOSS 3.554
Sillero 3.834
Patrickios 0.006
IPC_peptide 3.63
IPC2_peptide 3.821
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A4P8PQV5|A0A4P8PQV5_9ADEN ORF1 OS=White sturgeon adenovirus 1 OX=2580388 PE=4 SV=1
MM1 pKa = 7.44 YY2 pKa = 9.16 STGDD6 pKa = 3.25 STGWGRR12 pKa = 11.84 RR13 pKa = 11.84 VSQIRR18 pKa = 11.84 FSFQIFF24 pKa = 3.19
Molecular weight: 2.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.911
IPC_protein 11.038
Toseland 10.584
ProMoST 10.921
Dawson 10.789
Bjellqvist 10.73
Wikipedia 11.213
Rodwell 10.496
Grimsley 10.891
Solomon 11.111
Lehninger 11.038
Nozaki 10.555
DTASelect 10.745
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.745
Patrickios 10.716
IPC_peptide 11.096
IPC2_peptide 10.087
IPC2.peptide.svr19 8.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
14671
24
1166
305.6
34.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.855 ± 0.228
2.488 ± 0.269
5.323 ± 0.178
5.392 ± 0.343
4.792 ± 0.209
5.617 ± 0.336
2.331 ± 0.192
5.61 ± 0.257
5.398 ± 0.354
8.636 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.74 ± 0.138
6.032 ± 0.3
5.712 ± 0.315
4.015 ± 0.174
4.69 ± 0.339
7.232 ± 0.228
6.4 ± 0.277
5.916 ± 0.205
1.254 ± 0.117
4.567 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here