Amino acid dipepetide frequency for Neorhizobium alkalisoli

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.581AlaAla: 15.581 ± 0.121
0.928AlaCys: 0.928 ± 0.022
6.758AlaAsp: 6.758 ± 0.057
7.347AlaGlu: 7.347 ± 0.077
4.522AlaPhe: 4.522 ± 0.051
10.052AlaGly: 10.052 ± 0.083
2.056AlaHis: 2.056 ± 0.032
6.955AlaIle: 6.955 ± 0.063
4.668AlaLys: 4.668 ± 0.067
12.157AlaLeu: 12.157 ± 0.103
3.599AlaMet: 3.599 ± 0.039
3.094AlaAsn: 3.094 ± 0.049
4.684AlaPro: 4.684 ± 0.062
3.522AlaGln: 3.522 ± 0.053
7.052AlaArg: 7.052 ± 0.076
6.751AlaSer: 6.751 ± 0.059
5.89AlaThr: 5.89 ± 0.068
8.33AlaVal: 8.33 ± 0.072
1.302AlaTrp: 1.302 ± 0.026
2.615AlaTyr: 2.615 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.777CysAla: 0.777 ± 0.019
0.099CysCys: 0.099 ± 0.008
0.467CysAsp: 0.467 ± 0.016
0.417CysGlu: 0.417 ± 0.017
0.324CysPhe: 0.324 ± 0.012
0.852CysGly: 0.852 ± 0.019
0.222CysHis: 0.222 ± 0.01
0.407CysIle: 0.407 ± 0.014
0.189CysLys: 0.189 ± 0.01
0.795CysLeu: 0.795 ± 0.02
0.153CysMet: 0.153 ± 0.008
0.2CysAsn: 0.2 ± 0.01
0.342CysPro: 0.342 ± 0.013
0.22CysGln: 0.22 ± 0.011
0.576CysArg: 0.576 ± 0.018
0.449CysSer: 0.449 ± 0.016
0.337CysThr: 0.337 ± 0.014
0.55CysVal: 0.55 ± 0.018
0.098CysTrp: 0.098 ± 0.007
0.181CysTyr: 0.181 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.447AspAla: 6.447 ± 0.078
0.464AspCys: 0.464 ± 0.015
3.242AspAsp: 3.242 ± 0.05
3.468AspGlu: 3.468 ± 0.048
2.474AspPhe: 2.474 ± 0.04
5.203AspGly: 5.203 ± 0.082
1.272AspHis: 1.272 ± 0.032
3.588AspIle: 3.588 ± 0.049
2.047AspLys: 2.047 ± 0.035
6.026AspLeu: 6.026 ± 0.063
1.504AspMet: 1.504 ± 0.028
1.58AspAsn: 1.58 ± 0.033
3.241AspPro: 3.241 ± 0.045
1.789AspGln: 1.789 ± 0.03
4.115AspArg: 4.115 ± 0.047
2.094AspSer: 2.094 ± 0.04
2.724AspThr: 2.724 ± 0.068
4.135AspVal: 4.135 ± 0.043
0.949AspTrp: 0.949 ± 0.022
1.56AspTyr: 1.56 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.155GluAla: 7.155 ± 0.076
0.328GluCys: 0.328 ± 0.013
2.856GluAsp: 2.856 ± 0.043
3.415GluGlu: 3.415 ± 0.056
1.94GluPhe: 1.94 ± 0.031
4.128GluGly: 4.128 ± 0.049
1.136GluHis: 1.136 ± 0.023
3.935GluIle: 3.935 ± 0.047
3.014GluLys: 3.014 ± 0.044
5.217GluLeu: 5.217 ± 0.056
1.58GluMet: 1.58 ± 0.028
1.987GluAsn: 1.987 ± 0.032
2.512GluPro: 2.512 ± 0.038
2.092GluGln: 2.092 ± 0.037
4.38GluArg: 4.38 ± 0.061
2.266GluSer: 2.266 ± 0.034
3.729GluThr: 3.729 ± 0.043
3.604GluVal: 3.604 ± 0.043
0.677GluTrp: 0.677 ± 0.019
1.035GluTyr: 1.035 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
4.523PheAla: 4.523 ± 0.046
0.379PheCys: 0.379 ± 0.012
2.83PheAsp: 2.83 ± 0.038
2.277PheGlu: 2.277 ± 0.034
1.573PhePhe: 1.573 ± 0.03
3.815PheGly: 3.815 ± 0.049
0.782PheHis: 0.782 ± 0.022
1.982PheIle: 1.982 ± 0.031
1.257PheLys: 1.257 ± 0.024
3.581PheLeu: 3.581 ± 0.05
0.905PheMet: 0.905 ± 0.022
1.15PheAsn: 1.15 ± 0.026
1.653PhePro: 1.653 ± 0.031
1.079PheGln: 1.079 ± 0.023
2.323PheArg: 2.323 ± 0.035
2.793PheSer: 2.793 ± 0.035
2.069PheThr: 2.069 ± 0.043
2.906PheVal: 2.906 ± 0.041
0.561PheTrp: 0.561 ± 0.021
0.984PheTyr: 0.984 ± 0.022
0.0PheXaa: 0.0 ± 0.0
Gly
8.476GlyAla: 8.476 ± 0.082
0.72GlyCys: 0.72 ± 0.019
4.416GlyAsp: 4.416 ± 0.06
4.782GlyGlu: 4.782 ± 0.056
3.748GlyPhe: 3.748 ± 0.045
7.163GlyGly: 7.163 ± 0.092
1.842GlyHis: 1.842 ± 0.03
5.099GlyIle: 5.099 ± 0.061
3.853GlyLys: 3.853 ± 0.061
8.478GlyLeu: 8.478 ± 0.073
2.476GlyMet: 2.476 ± 0.04
2.609GlyAsn: 2.609 ± 0.053
3.178GlyPro: 3.178 ± 0.04
2.799GlyGln: 2.799 ± 0.043
5.544GlyArg: 5.544 ± 0.048
4.977GlySer: 4.977 ± 0.072
4.638GlyThr: 4.638 ± 0.105
5.664GlyVal: 5.664 ± 0.052
1.26GlyTrp: 1.26 ± 0.026
2.34GlyTyr: 2.34 ± 0.031
0.0GlyXaa: 0.0 ± 0.0
His
2.141HisAla: 2.141 ± 0.038
0.205HisCys: 0.205 ± 0.01
1.263HisAsp: 1.263 ± 0.025
1.085HisGlu: 1.085 ± 0.025
0.903HisPhe: 0.903 ± 0.024
1.854HisGly: 1.854 ± 0.032
0.547HisHis: 0.547 ± 0.023
0.995HisIle: 0.995 ± 0.022
0.541HisLys: 0.541 ± 0.016
1.983HisLeu: 1.983 ± 0.037
0.545HisMet: 0.545 ± 0.017
0.461HisAsn: 0.461 ± 0.015
1.207HisPro: 1.207 ± 0.025
0.603HisGln: 0.603 ± 0.018
1.23HisArg: 1.23 ± 0.028
1.012HisSer: 1.012 ± 0.021
0.745HisThr: 0.745 ± 0.022
1.482HisVal: 1.482 ± 0.028
0.293HisTrp: 0.293 ± 0.012
0.543HisTyr: 0.543 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.938IleAla: 7.938 ± 0.078
0.562IleCys: 0.562 ± 0.019
3.975IleAsp: 3.975 ± 0.047
3.824IleGlu: 3.824 ± 0.05
2.096IlePhe: 2.096 ± 0.035
5.564IleGly: 5.564 ± 0.06
0.955IleHis: 0.955 ± 0.022
2.756IleIle: 2.756 ± 0.037
1.707IleLys: 1.707 ± 0.036
5.086IleLeu: 5.086 ± 0.059
1.167IleMet: 1.167 ± 0.023
1.623IleAsn: 1.623 ± 0.034
2.522IlePro: 2.522 ± 0.033
1.305IleGln: 1.305 ± 0.027
3.482IleArg: 3.482 ± 0.041
3.744IleSer: 3.744 ± 0.045
3.011IleThr: 3.011 ± 0.052
4.647IleVal: 4.647 ± 0.049
0.664IleTrp: 0.664 ± 0.021
1.285IleTyr: 1.285 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
5.025LysAla: 5.025 ± 0.07
0.151LysCys: 0.151 ± 0.009
2.15LysAsp: 2.15 ± 0.041
1.823LysGlu: 1.823 ± 0.036
1.127LysPhe: 1.127 ± 0.026
3.003LysGly: 3.003 ± 0.046
0.648LysHis: 0.648 ± 0.02
2.238LysIle: 2.238 ± 0.033
1.642LysLys: 1.642 ± 0.035
3.974LysLeu: 3.974 ± 0.051
0.999LysMet: 0.999 ± 0.024
1.159LysAsn: 1.159 ± 0.027
2.459LysPro: 2.459 ± 0.041
1.236LysGln: 1.236 ± 0.03
2.451LysArg: 2.451 ± 0.035
2.357LysSer: 2.357 ± 0.034
2.47LysThr: 2.47 ± 0.04
2.759LysVal: 2.759 ± 0.043
0.382LysTrp: 0.382 ± 0.013
0.687LysTyr: 0.687 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
12.371LeuAla: 12.371 ± 0.093
0.838LeuCys: 0.838 ± 0.022
5.932LeuAsp: 5.932 ± 0.062
5.094LeuGlu: 5.094 ± 0.059
3.707LeuPhe: 3.707 ± 0.046
7.912LeuGly: 7.912 ± 0.071
1.739LeuHis: 1.739 ± 0.031
5.344LeuIle: 5.344 ± 0.066
4.264LeuLys: 4.264 ± 0.053
8.989LeuLeu: 8.989 ± 0.088
2.43LeuMet: 2.43 ± 0.041
2.715LeuAsn: 2.715 ± 0.034
5.197LeuPro: 5.197 ± 0.053
2.785LeuGln: 2.785 ± 0.036
5.874LeuArg: 5.874 ± 0.068
7.304LeuSer: 7.304 ± 0.082
5.565LeuThr: 5.565 ± 0.068
7.278LeuVal: 7.278 ± 0.065
1.076LeuTrp: 1.076 ± 0.025
2.062LeuTyr: 2.062 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.152MetAla: 3.152 ± 0.042
0.138MetCys: 0.138 ± 0.009
1.268MetAsp: 1.268 ± 0.026
1.22MetGlu: 1.22 ± 0.023
0.771MetPhe: 0.771 ± 0.02
1.745MetGly: 1.745 ± 0.032
0.425MetHis: 0.425 ± 0.015
1.604MetIle: 1.604 ± 0.029
1.184MetLys: 1.184 ± 0.021
2.646MetLeu: 2.646 ± 0.036
0.753MetMet: 0.753 ± 0.02
0.875MetAsn: 0.875 ± 0.021
1.613MetPro: 1.613 ± 0.028
0.926MetGln: 0.926 ± 0.023
1.838MetArg: 1.838 ± 0.034
1.872MetSer: 1.872 ± 0.032
2.061MetThr: 2.061 ± 0.033
1.79MetVal: 1.79 ± 0.034
0.181MetTrp: 0.181 ± 0.009
0.331MetTyr: 0.331 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.333AsnAla: 3.333 ± 0.044
0.239AsnCys: 0.239 ± 0.011
1.692AsnAsp: 1.692 ± 0.061
1.413AsnGlu: 1.413 ± 0.03
1.134AsnPhe: 1.134 ± 0.027
2.82AsnGly: 2.82 ± 0.071
0.556AsnHis: 0.556 ± 0.018
1.621AsnIle: 1.621 ± 0.028
0.825AsnLys: 0.825 ± 0.025
2.805AsnLeu: 2.805 ± 0.04
0.707AsnMet: 0.707 ± 0.018
0.82AsnAsn: 0.82 ± 0.021
1.91AsnPro: 1.91 ± 0.034
0.828AsnGln: 0.828 ± 0.019
1.926AsnArg: 1.926 ± 0.031
1.542AsnSer: 1.542 ± 0.031
1.425AsnThr: 1.425 ± 0.032
2.091AsnVal: 2.091 ± 0.033
0.446AsnTrp: 0.446 ± 0.014
0.722AsnTyr: 0.722 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
5.658ProAla: 5.658 ± 0.067
0.245ProCys: 0.245 ± 0.011
3.362ProAsp: 3.362 ± 0.045
3.4ProGlu: 3.4 ± 0.04
2.023ProPhe: 2.023 ± 0.033
3.986ProGly: 3.986 ± 0.048
1.024ProHis: 1.024 ± 0.025
2.529ProIle: 2.529 ± 0.035
1.977ProLys: 1.977 ± 0.035
4.484ProLeu: 4.484 ± 0.048
1.186ProMet: 1.186 ± 0.025
1.4ProAsn: 1.4 ± 0.028
2.082ProPro: 2.082 ± 0.042
1.656ProGln: 1.656 ± 0.031
2.391ProArg: 2.391 ± 0.039
2.851ProSer: 2.851 ± 0.04
2.411ProThr: 2.411 ± 0.036
4.144ProVal: 4.144 ± 0.053
0.579ProTrp: 0.579 ± 0.017
1.191ProTyr: 1.191 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.876GlnAla: 3.876 ± 0.048
0.155GlnCys: 0.155 ± 0.008
1.433GlnAsp: 1.433 ± 0.027
1.503GlnGlu: 1.503 ± 0.034
1.114GlnPhe: 1.114 ± 0.024
2.278GlnGly: 2.278 ± 0.034
0.628GlnHis: 0.628 ± 0.019
2.002GlnIle: 2.002 ± 0.043
1.313GlnLys: 1.313 ± 0.024
2.875GlnLeu: 2.875 ± 0.043
0.927GlnMet: 0.927 ± 0.023
0.965GlnAsn: 0.965 ± 0.022
1.76GlnPro: 1.76 ± 0.035
1.336GlnGln: 1.336 ± 0.037
2.085GlnArg: 2.085 ± 0.036
1.982GlnSer: 1.982 ± 0.036
1.727GlnThr: 1.727 ± 0.031
2.182GlnVal: 2.182 ± 0.039
0.385GlnTrp: 0.385 ± 0.014
0.665GlnTyr: 0.665 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
6.301ArgAla: 6.301 ± 0.07
0.419ArgCys: 0.419 ± 0.014
3.73ArgAsp: 3.73 ± 0.043
3.753ArgGlu: 3.753 ± 0.05
2.72ArgPhe: 2.72 ± 0.039
4.163ArgGly: 4.163 ± 0.053
1.561ArgHis: 1.561 ± 0.028
3.993ArgIle: 3.993 ± 0.046
2.52ArgLys: 2.52 ± 0.036
6.948ArgLeu: 6.948 ± 0.072
1.861ArgMet: 1.861 ± 0.034
1.922ArgAsn: 1.922 ± 0.03
3.076ArgPro: 3.076 ± 0.045
2.489ArgGln: 2.489 ± 0.04
4.747ArgArg: 4.747 ± 0.065
3.776ArgSer: 3.776 ± 0.048
3.036ArgThr: 3.036 ± 0.037
4.126ArgVal: 4.126 ± 0.049
0.857ArgTrp: 0.857 ± 0.02
1.748ArgTyr: 1.748 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.737SerAla: 6.737 ± 0.056
0.424SerCys: 0.424 ± 0.016
3.406SerAsp: 3.406 ± 0.055
3.139SerGlu: 3.139 ± 0.044
2.604SerPhe: 2.604 ± 0.041
5.973SerGly: 5.973 ± 0.062
1.206SerHis: 1.206 ± 0.028
3.341SerIle: 3.341 ± 0.045
1.959SerLys: 1.959 ± 0.033
5.979SerLeu: 5.979 ± 0.062
1.518SerMet: 1.518 ± 0.029
1.623SerAsn: 1.623 ± 0.029
2.856SerPro: 2.856 ± 0.037
1.855SerGln: 1.855 ± 0.031
3.766SerArg: 3.766 ± 0.046
3.632SerSer: 3.632 ± 0.054
3.058SerThr: 3.058 ± 0.059
4.412SerVal: 4.412 ± 0.057
0.763SerTrp: 0.763 ± 0.022
1.48SerTyr: 1.48 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
6.215ThrAla: 6.215 ± 0.08
0.397ThrCys: 0.397 ± 0.016
3.008ThrAsp: 3.008 ± 0.05
2.777ThrGlu: 2.777 ± 0.039
2.221ThrPhe: 2.221 ± 0.044
5.08ThrGly: 5.08 ± 0.074
0.96ThrHis: 0.96 ± 0.023
3.442ThrIle: 3.442 ± 0.072
1.825ThrLys: 1.825 ± 0.035
5.592ThrLeu: 5.592 ± 0.071
1.281ThrMet: 1.281 ± 0.023
1.462ThrAsn: 1.462 ± 0.034
3.025ThrPro: 3.025 ± 0.042
1.41ThrGln: 1.41 ± 0.028
2.996ThrArg: 2.996 ± 0.037
3.207ThrSer: 3.207 ± 0.048
2.919ThrThr: 2.919 ± 0.052
4.58ThrVal: 4.58 ± 0.103
0.64ThrTrp: 0.64 ± 0.021
1.337ThrTyr: 1.337 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
8.606ValAla: 8.606 ± 0.079
0.595ValCys: 0.595 ± 0.018
3.967ValAsp: 3.967 ± 0.044
4.472ValGlu: 4.472 ± 0.049
2.891ValPhe: 2.891 ± 0.042
5.378ValGly: 5.378 ± 0.057
1.329ValHis: 1.329 ± 0.024
4.383ValIle: 4.383 ± 0.05
2.707ValLys: 2.707 ± 0.039
7.043ValLeu: 7.043 ± 0.057
1.974ValMet: 1.974 ± 0.031
2.081ValAsn: 2.081 ± 0.051
3.488ValPro: 3.488 ± 0.047
1.932ValGln: 1.932 ± 0.031
4.29ValArg: 4.29 ± 0.049
4.92ValSer: 4.92 ± 0.06
4.686ValThr: 4.686 ± 0.092
5.671ValVal: 5.671 ± 0.057
0.831ValTrp: 0.831 ± 0.022
1.503ValTyr: 1.503 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.079TrpAla: 1.079 ± 0.022
0.125TrpCys: 0.125 ± 0.008
0.61TrpAsp: 0.61 ± 0.019
0.529TrpGlu: 0.529 ± 0.016
0.531TrpPhe: 0.531 ± 0.017
0.854TrpGly: 0.854 ± 0.02
0.299TrpHis: 0.299 ± 0.013
0.666TrpIle: 0.666 ± 0.017
0.542TrpLys: 0.542 ± 0.016
1.517TrpLeu: 1.517 ± 0.031
0.354TrpMet: 0.354 ± 0.015
0.473TrpAsn: 0.473 ± 0.017
0.644TrpPro: 0.644 ± 0.017
0.559TrpGln: 0.559 ± 0.02
0.969TrpArg: 0.969 ± 0.022
0.821TrpSer: 0.821 ± 0.022
0.697TrpThr: 0.697 ± 0.02
0.739TrpVal: 0.739 ± 0.021
0.21TrpTrp: 0.21 ± 0.013
0.292TrpTyr: 0.292 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.47TyrAla: 2.47 ± 0.033
0.244TyrCys: 0.244 ± 0.012
1.557TyrAsp: 1.557 ± 0.026
1.249TyrGlu: 1.249 ± 0.031
0.998TyrPhe: 0.998 ± 0.023
2.244TyrGly: 2.244 ± 0.032
0.454TyrHis: 0.454 ± 0.016
1.033TyrIle: 1.033 ± 0.023
0.704TyrLys: 0.704 ± 0.019
2.298TyrLeu: 2.298 ± 0.035
0.471TyrMet: 0.471 ± 0.017
0.657TyrAsn: 0.657 ± 0.021
1.129TyrPro: 1.129 ± 0.027
0.769TyrGln: 0.769 ± 0.021
1.712TyrArg: 1.712 ± 0.03
1.381TyrSer: 1.381 ± 0.027
1.179TyrThr: 1.179 ± 0.041
1.657TyrVal: 1.657 ± 0.03
0.356TyrTrp: 0.356 ± 0.013
0.629TyrTyr: 0.629 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6627 proteins (2057094 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski