Mycobacterium virus Babsiella

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chebruvirinae; Brujitavirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8I6Q7|G8I6Q7_9CAUD Uncharacterized protein OS=Mycobacterium virus Babsiella OX=1089115 GN=24 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 5.87LFVPCPLCGSDD13 pKa = 3.44VAVPLLSEE21 pKa = 4.46GDD23 pKa = 3.7HH24 pKa = 6.42VRR26 pKa = 11.84APDD29 pKa = 4.25LLPNMNSHH37 pKa = 6.92VISVHH42 pKa = 5.19EE43 pKa = 4.08MDD45 pKa = 3.8VSGMM49 pKa = 3.65

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8I6R7|G8I6R7_9CAUD Uncharacterized protein OS=Mycobacterium virus Babsiella OX=1089115 GN=34 PE=4 SV=1
MM1 pKa = 7.82LLEE4 pKa = 4.52QKK6 pKa = 10.65FSPQSVGSVSRR17 pKa = 11.84VHH19 pKa = 7.21GPMVNRR25 pKa = 11.84WRR27 pKa = 11.84TWQFAQSLSRR37 pKa = 11.84RR38 pKa = 11.84TVEE41 pKa = 3.71EE42 pKa = 3.55RR43 pKa = 11.84VATVRR48 pKa = 11.84RR49 pKa = 11.84MAEE52 pKa = 3.56WCGVSPEE59 pKa = 4.09FAQVEE64 pKa = 4.5QIVEE68 pKa = 3.97WLAEE72 pKa = 4.18GGTWSARR79 pKa = 11.84TRR81 pKa = 11.84WTYY84 pKa = 11.34YY85 pKa = 10.31GALSAWFLWLQQQGYY100 pKa = 8.47RR101 pKa = 11.84QDD103 pKa = 3.5NPMVMIGRR111 pKa = 11.84PKK113 pKa = 10.09RR114 pKa = 11.84PKK116 pKa = 9.75SVPRR120 pKa = 11.84PVSNLDD126 pKa = 3.3MQRR129 pKa = 11.84LLAVRR134 pKa = 11.84AHH136 pKa = 6.47KK137 pKa = 9.25RR138 pKa = 11.84TKK140 pKa = 11.33AMILLAAFQGLRR152 pKa = 11.84VHH154 pKa = 7.42EE155 pKa = 4.17IAQVKK160 pKa = 10.12GEE162 pKa = 4.11HH163 pKa = 6.82LDD165 pKa = 4.08LIEE168 pKa = 4.13RR169 pKa = 11.84TMTVTGKK176 pKa = 11.18GNITATLPLHH186 pKa = 5.86HH187 pKa = 6.81RR188 pKa = 11.84VVEE191 pKa = 4.17MAYY194 pKa = 9.4QMPRR198 pKa = 11.84KK199 pKa = 9.17GHH201 pKa = 6.4WFPGPDD207 pKa = 2.81RR208 pKa = 11.84GHH210 pKa = 5.51QRR212 pKa = 11.84RR213 pKa = 11.84EE214 pKa = 4.31SVSGTIKK221 pKa = 10.28EE222 pKa = 4.01AMIRR226 pKa = 11.84AGVLGSAHH234 pKa = 7.05CLRR237 pKa = 11.84HH238 pKa = 4.94WFGTALLEE246 pKa = 4.69AGVDD250 pKa = 3.56LRR252 pKa = 11.84TVQEE256 pKa = 4.11LMRR259 pKa = 11.84HH260 pKa = 4.7QSLTSTEE267 pKa = 3.62IYY269 pKa = 10.3TRR271 pKa = 11.84VTDD274 pKa = 3.31QRR276 pKa = 11.84RR277 pKa = 11.84AEE279 pKa = 4.72GIQRR283 pKa = 11.84LDD285 pKa = 3.27PFGVARR291 pKa = 11.84QGHH294 pKa = 5.86KK295 pKa = 10.41AADD298 pKa = 3.78IIGEE302 pKa = 4.19MIADD306 pKa = 4.18EE307 pKa = 4.42VAGSGASSAAA317 pKa = 3.37

Molecular weight: 35.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15845

40

1255

203.1

22.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.562 ± 0.499

0.947 ± 0.129

6.854 ± 0.244

6.015 ± 0.275

2.979 ± 0.184

9.183 ± 0.554

2.057 ± 0.176

4.361 ± 0.167

3.238 ± 0.22

7.889 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.133

3.08 ± 0.192

6.191 ± 0.202

3.812 ± 0.247

7.176 ± 0.492

5.308 ± 0.262

5.92 ± 0.298

6.835 ± 0.248

2.045 ± 0.122

2.398 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski