Acinetobacter phage Petty
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5PVH4|U5PVH4_9CAUD Uncharacterized protein OS=Acinetobacter phage Petty OX=1406779 GN=Petty_8 PE=4 SV=1
MM1 pKa = 7.43 TFYY4 pKa = 10.93 DD5 pKa = 3.92 YY6 pKa = 11.1 VTTQRR11 pKa = 11.84 ICDD14 pKa = 3.95 AFVDD18 pKa = 4.43 DD19 pKa = 4.85 YY20 pKa = 12.09 GNLVPLNSSTYY31 pKa = 8.27 FTWNLWADD39 pKa = 3.77 FDD41 pKa = 4.31 HH42 pKa = 7.07 YY43 pKa = 11.78 YY44 pKa = 10.7 HH45 pKa = 6.2 GTSRR49 pKa = 11.84 TMLGAA54 pKa = 4.22
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.239
IPC2_protein 4.368
IPC_protein 4.164
Toseland 3.948
ProMoST 4.406
Dawson 4.215
Bjellqvist 4.38
Wikipedia 4.253
Rodwell 4.012
Grimsley 3.872
Solomon 4.202
Lehninger 4.151
Nozaki 4.368
DTASelect 4.698
Thurlkill 4.062
EMBOSS 4.253
Sillero 4.317
Patrickios 0.095
IPC_peptide 4.19
IPC2_peptide 4.279
IPC2.peptide.svr19 4.205
Protein with the highest isoelectric point:
>tr|U5PVS5|U5PVS5_9CAUD Capsid protein OS=Acinetobacter phage Petty OX=1406779 GN=Petty_32 PE=4 SV=1
MM1 pKa = 7.71 LISIIVLAILVVSAVATTVYY21 pKa = 10.21 RR22 pKa = 11.84 SPKK25 pKa = 9.95 IKK27 pKa = 10.47 VAWAEE32 pKa = 3.68 YY33 pKa = 8.64 VALVKK38 pKa = 11.18 SMGTKK43 pKa = 10.4 NFLAEE48 pKa = 4.41 CKK50 pKa = 10.23 DD51 pKa = 3.84 DD52 pKa = 6.1 LKK54 pKa = 11.27 HH55 pKa = 5.67 SWNVTRR61 pKa = 11.84 KK62 pKa = 10.2 AFGNLWQAFKK72 pKa = 11.12 LVGLWLSVPFIVFIGLPLVLLLVCLALTFVPNRR105 pKa = 11.84 RR106 pKa = 11.84 II107 pKa = 3.34
Molecular weight: 11.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.139
IPC2_protein 9.37
IPC_protein 9.355
Toseland 10.292
ProMoST 9.823
Dawson 10.409
Bjellqvist 10.028
Wikipedia 10.526
Rodwell 11.052
Grimsley 10.438
Solomon 10.438
Lehninger 10.423
Nozaki 10.292
DTASelect 9.999
Thurlkill 10.292
EMBOSS 10.672
Sillero 10.321
Patrickios 10.847
IPC_peptide 10.452
IPC2_peptide 8.726
IPC2.peptide.svr19 8.318
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
12393
24
1055
275.4
30.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.594 ± 0.454
1.178 ± 0.163
5.995 ± 0.187
6.213 ± 0.257
3.462 ± 0.203
6.883 ± 0.325
2.171 ± 0.205
5.366 ± 0.218
6.124 ± 0.292
8.65 ± 0.31
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.175
5.027 ± 0.242
3.671 ± 0.246
4.406 ± 0.33
4.995 ± 0.191
5.874 ± 0.245
5.963 ± 0.283
7.044 ± 0.293
1.267 ± 0.136
4.398 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here