Brumimicrobium salinarum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Crocinitomicaceae; Brumimicrobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0QYS6|A0A2I0QYS6_9FLAO Uncharacterized protein OS=Brumimicrobium salinarum OX=2058658 GN=CW751_14995 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.42NLYY5 pKa = 10.42LFFAATILTFTANTQNFNEE24 pKa = 4.36VNPSPFVDD32 pKa = 3.09IGRR35 pKa = 11.84GKK37 pKa = 10.66SVMADD42 pKa = 3.12VDD44 pKa = 3.9NDD46 pKa = 3.4GHH48 pKa = 8.74LDD50 pKa = 3.66IIIAGNSYY58 pKa = 10.97EE59 pKa = 5.12NSTQTDD65 pKa = 3.54TVIVYY70 pKa = 10.64RR71 pKa = 11.84NDD73 pKa = 3.05GTGNYY78 pKa = 8.74TEE80 pKa = 4.04ITGNSFLGVYY90 pKa = 10.02RR91 pKa = 11.84PAFDD95 pKa = 4.53IADD98 pKa = 3.69IDD100 pKa = 4.3DD101 pKa = 5.41DD102 pKa = 5.08GDD104 pKa = 3.54IDD106 pKa = 4.94ILITGANNGSLTAKK120 pKa = 10.53LYY122 pKa = 10.63INDD125 pKa = 3.52GTGIFTEE132 pKa = 4.51VVTTIFEE139 pKa = 4.17AVEE142 pKa = 3.91NGTVNFADD150 pKa = 3.64VDD152 pKa = 3.75NDD154 pKa = 3.28GDD156 pKa = 4.06QDD158 pKa = 3.87VLLTGRR164 pKa = 11.84NNNATSISKK173 pKa = 10.41LYY175 pKa = 10.23INNGSGNFTEE185 pKa = 4.47NTGSPFTGVRR195 pKa = 11.84NGVVEE200 pKa = 4.3FADD203 pKa = 3.53INGNGSLDD211 pKa = 3.67LLINGEE217 pKa = 4.35LYY219 pKa = 10.62INDD222 pKa = 3.51GNGNFSLQTPANLTYY237 pKa = 10.53FGYY240 pKa = 11.06GDD242 pKa = 4.06AAFEE246 pKa = 5.82DD247 pKa = 4.06IDD249 pKa = 4.55NDD251 pKa = 3.68GDD253 pKa = 4.15LDD255 pKa = 4.96LIITGSMPNQIQPIYY270 pKa = 9.58TKK272 pKa = 10.71LYY274 pKa = 9.6LNDD277 pKa = 3.5GTGVFTEE284 pKa = 3.92ITGTLFTNLRR294 pKa = 11.84YY295 pKa = 10.17SSVDD299 pKa = 3.97FIDD302 pKa = 4.59FNGDD306 pKa = 3.39SYY308 pKa = 11.89PDD310 pKa = 3.78LLIGGVDD317 pKa = 3.6TNGDD321 pKa = 2.9KK322 pKa = 10.99HH323 pKa = 6.08ATFFINDD330 pKa = 3.23GSGNFTIAPQQPIEE344 pKa = 4.11AFSNGSLSIGDD355 pKa = 3.47VDD357 pKa = 4.5GDD359 pKa = 3.75LDD361 pKa = 4.51KK362 pKa = 11.54DD363 pKa = 3.9VLITGLYY370 pKa = 7.53EE371 pKa = 4.14KK372 pKa = 10.28PSVFGGTVTARR383 pKa = 11.84ITRR386 pKa = 11.84LYY388 pKa = 10.14KK389 pKa = 10.57LCNLEE394 pKa = 4.76LDD396 pKa = 3.92QPTLNPISAICSLDD410 pKa = 3.6EE411 pKa = 4.33PQAPTATDD419 pKa = 3.11NCSS422 pKa = 2.77

Molecular weight: 45.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0R2R8|A0A2I0R2R8_9FLAO S9 family peptidase OS=Brumimicrobium salinarum OX=2058658 GN=CW751_06830 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.56HH16 pKa = 3.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.87GRR39 pKa = 11.84KK40 pKa = 9.0RR41 pKa = 11.84LTVSSNKK48 pKa = 9.31LAKK51 pKa = 10.16RR52 pKa = 3.58

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2930

0

2930

972125

25

2495

331.8

37.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.027 ± 0.043

0.801 ± 0.018

5.637 ± 0.037

6.855 ± 0.05

5.227 ± 0.037

6.467 ± 0.052

1.879 ± 0.02

7.891 ± 0.043

7.675 ± 0.064

8.995 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.024

6.216 ± 0.045

3.424 ± 0.027

3.543 ± 0.03

3.494 ± 0.034

6.627 ± 0.042

5.737 ± 0.057

6.187 ± 0.033

1.028 ± 0.015

4.054 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski