Lactobacillus phage JNU_P11
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M3BEU5|A0A6M3BEU5_9CAUD Antirepressor OS=Lactobacillus phage JNU_P11 OX=2686381 PE=4 SV=1
MM1 pKa = 8.2 PDD3 pKa = 3.88 LLLEE7 pKa = 4.36 IYY9 pKa = 10.31 KK10 pKa = 10.27 EE11 pKa = 3.88 QLDD14 pKa = 4.15 WGWITLDD21 pKa = 3.8 DD22 pKa = 4.41 LKK24 pKa = 11.72 ANVNSGLLAPDD35 pKa = 4.38 DD36 pKa = 4.04 FEE38 pKa = 7.17 KK39 pKa = 10.69 IAGQTYY45 pKa = 8.94 VAA47 pKa = 4.9
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.254
IPC2_protein 3.884
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 3.592
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A6M3BEU7|A0A6M3BEU7_9CAUD M23 family peptidase OS=Lactobacillus phage JNU_P11 OX=2686381 PE=4 SV=1
MM1 pKa = 7.67 RR2 pKa = 11.84 VNFTIEE8 pKa = 3.93 GPPIGKK14 pKa = 9.4 ARR16 pKa = 11.84 PRR18 pKa = 11.84 VTRR21 pKa = 11.84 TVTYY25 pKa = 9.63 TPAKK29 pKa = 7.16 TARR32 pKa = 11.84 YY33 pKa = 8.04 EE34 pKa = 3.93 DD35 pKa = 3.92 LVRR38 pKa = 11.84 YY39 pKa = 6.81 TAINSFKK46 pKa = 11.02 GIFDD50 pKa = 3.55 KK51 pKa = 11.36 DD52 pKa = 3.27 EE53 pKa = 4.55 PLDD56 pKa = 4.02 VKK58 pKa = 10.87 IMAYY62 pKa = 10.43 FEE64 pKa = 4.51 VPKK67 pKa = 10.8 SLSKK71 pKa = 10.38 KK72 pKa = 10.06 RR73 pKa = 11.84 KK74 pKa = 9.02 ALCLSNQEE82 pKa = 4.65 LPTKK86 pKa = 10.58 KK87 pKa = 9.86 PDD89 pKa = 3.42 ADD91 pKa = 3.63 NVGKK95 pKa = 10.45 IIMDD99 pKa = 3.8 GMNPKK104 pKa = 9.3 MKK106 pKa = 9.49 RR107 pKa = 11.84 DD108 pKa = 3.49 KK109 pKa = 10.41 RR110 pKa = 11.84 LHH112 pKa = 6.12 KK113 pKa = 9.64 MVEE116 pKa = 4.02 VMRR119 pKa = 11.84 GVYY122 pKa = 10.07 HH123 pKa = 7.97 DD124 pKa = 4.84 DD125 pKa = 3.64 KK126 pKa = 11.4 QVTTLLVKK134 pKa = 10.3 KK135 pKa = 10.07 RR136 pKa = 11.84 YY137 pKa = 9.48 AKK139 pKa = 10.2 RR140 pKa = 11.84 ARR142 pKa = 11.84 VDD144 pKa = 3.25 VRR146 pKa = 11.84 IKK148 pKa = 10.82 RR149 pKa = 11.84 DD150 pKa = 3.18 MGDD153 pKa = 2.8
Molecular weight: 17.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.138
IPC2_protein 9.443
IPC_protein 9.458
Toseland 10.379
ProMoST 9.911
Dawson 10.482
Bjellqvist 10.087
Wikipedia 10.613
Rodwell 11.052
Grimsley 10.526
Solomon 10.511
Lehninger 10.496
Nozaki 10.335
DTASelect 10.087
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.394
Patrickios 10.774
IPC_peptide 10.526
IPC2_peptide 8.551
IPC2.peptide.svr19 8.556
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11261
43
1134
204.7
23.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.14 ± 0.62
0.613 ± 0.098
7.22 ± 0.423
5.745 ± 0.367
3.739 ± 0.206
6.136 ± 0.338
1.492 ± 0.22
6.98 ± 0.357
8.862 ± 0.401
7.921 ± 0.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.415 ± 0.144
6.287 ± 0.311
2.904 ± 0.222
4.6 ± 0.218
3.659 ± 0.243
6.056 ± 0.289
6.03 ± 0.314
6.403 ± 0.289
1.705 ± 0.19
4.094 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here