Anncaliia algerae PRA339

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Tubulinosematoidea; Tubulinosematidae; Anncaliia; Anncaliia algerae

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059EWI3|A0A059EWI3_9MICR DDE_Tnp_IS1595 domain-containing protein OS=Anncaliia algerae PRA339 OX=1288291 GN=H312_03202 PE=4 SV=1
MM1 pKa = 7.78PEE3 pKa = 3.78MVIILSTDD11 pKa = 3.21NEE13 pKa = 4.41ILTSHH18 pKa = 7.36AANIKK23 pKa = 10.49DD24 pKa = 4.0EE25 pKa = 4.31EE26 pKa = 4.33EE27 pKa = 3.79MVYY30 pKa = 10.02MNEE33 pKa = 5.17LIDD36 pKa = 3.67EE37 pKa = 4.37TLIMIKK43 pKa = 10.1NAPIPEE49 pKa = 5.0DD50 pKa = 3.61DD51 pKa = 3.39QKK53 pKa = 11.53FFYY56 pKa = 10.53GFNNTSPLVSVYY68 pKa = 10.4ILSDD72 pKa = 3.18GMKK75 pKa = 9.8ILLFTEE81 pKa = 4.94DD82 pKa = 3.58KK83 pKa = 11.32NSDD86 pKa = 3.37EE87 pKa = 4.93VYY89 pKa = 11.01DD90 pKa = 4.6LLVDD94 pKa = 5.72LYY96 pKa = 11.51DD97 pKa = 4.33CFKK100 pKa = 11.23NDD102 pKa = 3.3YY103 pKa = 11.13FMDD106 pKa = 4.37NDD108 pKa = 4.45DD109 pKa = 4.86FPDD112 pKa = 4.0NEE114 pKa = 4.36PSNDD118 pKa = 3.1QGPYY122 pKa = 9.74YY123 pKa = 10.8GVARR127 pKa = 4.44

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059EZ16|A0A059EZ16_9MICR EamA domain-containing protein OS=Anncaliia algerae PRA339 OX=1288291 GN=H312_02452 PE=4 SV=1
NN1 pKa = 7.18ISRR4 pKa = 11.84VPVCRR9 pKa = 11.84RR10 pKa = 11.84LSHH13 pKa = 5.08MQIIRR18 pKa = 11.84KK19 pKa = 8.86RR20 pKa = 11.84LSLIPEE26 pKa = 3.87EE27 pKa = 6.05LIFQDD32 pKa = 5.09KK33 pKa = 9.95IDD35 pKa = 3.69ARR37 pKa = 11.84ATYY40 pKa = 9.77ASEE43 pKa = 4.37IIRR46 pKa = 11.84LLRR49 pKa = 11.84EE50 pKa = 3.69NLVFF54 pKa = 5.12

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3576

0

3576

953739

21

3183

266.7

31.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.968 ± 0.039

1.806 ± 0.019

5.062 ± 0.031

7.71 ± 0.049

6.335 ± 0.047

3.426 ± 0.04

1.783 ± 0.015

9.815 ± 0.042

10.594 ± 0.061

10.092 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.016

8.167 ± 0.056

2.426 ± 0.029

2.586 ± 0.029

3.48 ± 0.026

7.218 ± 0.048

4.525 ± 0.028

4.684 ± 0.03

0.55 ± 0.012

4.696 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski