Changjiang tombus-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFT6|A0A1L3KFT6_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 12 OX=1922805 PE=4 SV=1
MM1 pKa = 7.78PEE3 pKa = 3.67VSVTATGYY11 pKa = 10.14SKK13 pKa = 10.82KK14 pKa = 10.54APKK17 pKa = 9.73QVLSVLGLPSKK28 pKa = 10.47GQTWTHH34 pKa = 6.26ANDD37 pKa = 3.15VANVVEE43 pKa = 4.83SVHH46 pKa = 5.96EE47 pKa = 4.1RR48 pKa = 11.84VLGRR52 pKa = 11.84TADD55 pKa = 4.05GTWEE59 pKa = 3.71RR60 pKa = 11.84TMQPEE65 pKa = 4.04KK66 pKa = 11.0GAFTGDD72 pKa = 3.21LLVFRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84VGIHH83 pKa = 6.12TGSSSLPWSTEE94 pKa = 3.37QFVDD98 pKa = 4.14HH99 pKa = 6.69YY100 pKa = 10.65RR101 pKa = 11.84GQKK104 pKa = 7.92KK105 pKa = 9.78ARR107 pKa = 11.84YY108 pKa = 8.38QSAAKK113 pKa = 10.19SLEE116 pKa = 3.69EE117 pKa = 4.13RR118 pKa = 11.84PLSRR122 pKa = 11.84KK123 pKa = 9.75DD124 pKa = 3.4SYY126 pKa = 11.6PSVFLKK132 pKa = 10.69AEE134 pKa = 3.84KK135 pKa = 9.47WHH137 pKa = 6.71DD138 pKa = 3.71PKK140 pKa = 11.09AGRR143 pKa = 11.84LISARR148 pKa = 11.84HH149 pKa = 4.91PRR151 pKa = 11.84YY152 pKa = 9.92NLEE155 pKa = 4.03LGKK158 pKa = 10.61FILPLEE164 pKa = 4.48HH165 pKa = 6.9EE166 pKa = 4.74LYY168 pKa = 10.6KK169 pKa = 11.02AIDD172 pKa = 3.81EE173 pKa = 4.8VYY175 pKa = 10.92GSATIMKK182 pKa = 9.88GYY184 pKa = 8.78TPEE187 pKa = 3.41QRR189 pKa = 11.84AAVVEE194 pKa = 4.07QHH196 pKa = 5.82WGAFDD201 pKa = 3.71SPVAIGQDD209 pKa = 3.48YY210 pKa = 11.4SKK212 pKa = 10.95FDD214 pKa = 3.24QHH216 pKa = 7.35ISKK219 pKa = 10.63DD220 pKa = 3.35ALQYY224 pKa = 9.66EE225 pKa = 4.59HH226 pKa = 7.05GFYY229 pKa = 10.63LHH231 pKa = 7.47AYY233 pKa = 9.36GGDD236 pKa = 3.56EE237 pKa = 3.79NLQRR241 pKa = 11.84LLSWQLEE248 pKa = 4.4SRR250 pKa = 11.84CFANVQDD257 pKa = 4.87GVVQYY262 pKa = 8.67PVKK265 pKa = 9.93GGRR268 pKa = 11.84MSGDD272 pKa = 3.18MNTALGNCVISASLIWAYY290 pKa = 10.62ARR292 pKa = 11.84EE293 pKa = 4.16IGVKK297 pKa = 9.59IRR299 pKa = 11.84LVVDD303 pKa = 3.93GDD305 pKa = 3.95DD306 pKa = 3.37SVAFMEE312 pKa = 4.55RR313 pKa = 11.84TDD315 pKa = 3.4APRR318 pKa = 11.84YY319 pKa = 7.53QAGIKK324 pKa = 9.45EE325 pKa = 3.97WMARR329 pKa = 11.84RR330 pKa = 11.84GFRR333 pKa = 11.84LVSEE337 pKa = 4.37EE338 pKa = 3.84PVYY341 pKa = 10.7QINQVEE347 pKa = 4.99FCQCRR352 pKa = 11.84YY353 pKa = 10.58VGTTPPTMVRR363 pKa = 11.84NPLKK367 pKa = 10.77AITQDD372 pKa = 3.64HH373 pKa = 6.43AWVEE377 pKa = 4.55DD378 pKa = 3.57KK379 pKa = 11.0ALRR382 pKa = 11.84WADD385 pKa = 3.36VLAATGMGGLALYY398 pKa = 10.8GNIPLLGAYY407 pKa = 10.45YY408 pKa = 11.12DD409 pKa = 3.65MLARR413 pKa = 11.84ASEE416 pKa = 4.46VSAKK420 pKa = 9.49TLARR424 pKa = 11.84LDD426 pKa = 4.0TRR428 pKa = 11.84SSWLRR433 pKa = 11.84DD434 pKa = 3.2ATFGGVRR441 pKa = 11.84MEE443 pKa = 4.19PSEE446 pKa = 3.87MARR449 pKa = 11.84YY450 pKa = 8.8QFWLSWGMEE459 pKa = 3.71PGAQRR464 pKa = 11.84EE465 pKa = 4.25HH466 pKa = 6.48EE467 pKa = 4.16ARR469 pKa = 11.84FEE471 pKa = 4.63GIDD474 pKa = 3.78LSALKK479 pKa = 10.63AVDD482 pKa = 3.29TTKK485 pKa = 10.32IKK487 pKa = 10.64HH488 pKa = 5.59SSFYY492 pKa = 10.57DD493 pKa = 3.21TDD495 pKa = 3.07QAYY498 pKa = 10.74YY499 pKa = 9.97II500 pKa = 4.55

Molecular weight: 56.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFT6|A0A1L3KFT6_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 12 OX=1922805 PE=4 SV=1
MM1 pKa = 7.06VKK3 pKa = 10.15RR4 pKa = 11.84RR5 pKa = 11.84GSKK8 pKa = 10.17RR9 pKa = 11.84KK10 pKa = 7.64TNAPRR15 pKa = 11.84MRR17 pKa = 11.84NAPLAVRR24 pKa = 11.84APRR27 pKa = 11.84PRR29 pKa = 11.84MVSNEE34 pKa = 3.82EE35 pKa = 4.06KK36 pKa = 10.51LLKK39 pKa = 10.65LIMDD43 pKa = 4.46PCGAEE48 pKa = 3.96MAHH51 pKa = 6.88GYY53 pKa = 10.21ALSTEE58 pKa = 4.42GVVQRR63 pKa = 11.84FTQYY67 pKa = 7.69FTPTATTEE75 pKa = 3.86TSFAYY80 pKa = 10.15AINPAYY86 pKa = 10.06YY87 pKa = 10.55GPGGVNQVLGTGTGAIGTAISAPLPGQTFVDD118 pKa = 4.12ANADD122 pKa = 3.95TVSTLAACVEE132 pKa = 4.12VLYY135 pKa = 10.64TGTVVNRR142 pKa = 11.84KK143 pKa = 9.57GYY145 pKa = 9.7IAVAQGPSEE154 pKa = 4.2QIYY157 pKa = 8.2STITTPTVTFNGLVTLSNAVAPVPSAAVSVKK188 pKa = 7.43WTPPVANFIGSASAIEE204 pKa = 4.27TDD206 pKa = 2.86SHH208 pKa = 5.79YY209 pKa = 10.81QSNSILVTAVGVNPNDD225 pKa = 3.17FVVRR229 pKa = 11.84VTVVYY234 pKa = 9.85EE235 pKa = 3.72YY236 pKa = 11.13APKK239 pKa = 10.63VGLGMPLARR248 pKa = 11.84ATRR251 pKa = 11.84VTPVGVGEE259 pKa = 5.3RR260 pKa = 11.84ITSTLDD266 pKa = 3.34RR267 pKa = 11.84MGHH270 pKa = 4.67WWHH273 pKa = 6.5NLGDD277 pKa = 3.79AAAAASRR284 pKa = 11.84LGNRR288 pKa = 11.84VVYY291 pKa = 10.14GAGQVAGFTRR301 pKa = 11.84GLLRR305 pKa = 11.84TTEE308 pKa = 3.89AATTLLALAGG318 pKa = 3.87

Molecular weight: 33.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

818

318

500

409.0

44.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.002 ± 1.321

0.733 ± 0.063

4.279 ± 1.244

5.257 ± 1.077

2.934 ± 0.251

8.435 ± 0.598

2.2 ± 0.565

3.545 ± 0.052

4.156 ± 0.983

7.579 ± 0.396

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.146

3.056 ± 0.995

5.012 ± 0.765

3.79 ± 0.763

6.601 ± 0.375

6.479 ± 0.49

7.335 ± 2.199

8.802 ± 1.509

1.956 ± 0.607

4.401 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski