Rhodobacteraceae bacterium W635

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372IHV2|A0A372IHV2_9RHOB DoxX family protein OS=Rhodobacteraceae bacterium W635 OX=2303578 GN=DZD18_01035 PE=4 SV=1
MM1 pKa = 7.56LKK3 pKa = 10.45KK4 pKa = 10.73FILSEE9 pKa = 4.21SGAVTTDD16 pKa = 3.25YY17 pKa = 11.94VMLSASVVGMGIAVLNSTAAGVEE40 pKa = 4.17TLADD44 pKa = 5.12DD45 pKa = 4.43INTALTGQIVNTNFARR61 pKa = 11.84AAYY64 pKa = 9.96FDD66 pKa = 4.71DD67 pKa = 4.64FEE69 pKa = 6.09SGSGFWVGGRR79 pKa = 11.84TDD81 pKa = 4.01DD82 pKa = 4.64SEE84 pKa = 4.06EE85 pKa = 4.42AYY87 pKa = 10.5GGILGPYY94 pKa = 8.67GGTGGAEE101 pKa = 4.1VVTRR105 pKa = 11.84TYY107 pKa = 11.7DD108 pKa = 3.19VMSDD112 pKa = 3.2YY113 pKa = 11.75DD114 pKa = 3.68HH115 pKa = 7.13AVVEE119 pKa = 4.72FDD121 pKa = 3.6FHH123 pKa = 9.24AIDD126 pKa = 3.26SWDD129 pKa = 3.58NEE131 pKa = 4.13DD132 pKa = 5.04FIVFIDD138 pKa = 3.97GTPVSSHH145 pKa = 5.72NFDD148 pKa = 3.39WQSDD152 pKa = 3.88GTTGGWTTTEE162 pKa = 3.88ANYY165 pKa = 10.68SIAIEE170 pKa = 3.93PSGPRR175 pKa = 11.84QHH177 pKa = 6.91SGYY180 pKa = 10.11NSEE183 pKa = 4.42WTDD186 pKa = 2.83QSYY189 pKa = 10.6SVRR192 pKa = 11.84VEE194 pKa = 3.97VADD197 pKa = 4.83PGPSMSVGFGSTLNQDD213 pKa = 2.84IHH215 pKa = 7.56DD216 pKa = 4.48EE217 pKa = 4.07SWAVDD222 pKa = 3.6NVGVTSTNDD231 pKa = 3.29PNEE234 pKa = 3.96VV235 pKa = 3.06

Molecular weight: 25.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372IDH2|A0A372IDH2_9RHOB ABC transporter ATP-binding protein OS=Rhodobacteraceae bacterium W635 OX=2303578 GN=DZD18_09255 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.51ILNARR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.14SLSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3286

0

3286

1037509

22

2116

315.7

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.932 ± 0.065

0.891 ± 0.014

6.355 ± 0.034

5.892 ± 0.038

3.57 ± 0.027

9.027 ± 0.039

2.164 ± 0.021

4.851 ± 0.033

2.518 ± 0.037

10.134 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.02

2.292 ± 0.022

5.337 ± 0.034

2.983 ± 0.023

7.287 ± 0.04

4.687 ± 0.026

5.442 ± 0.028

7.298 ± 0.035

1.411 ± 0.018

2.13 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski