Gordonia phage Kiko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IL25|A0A3T0IL25_9CAUD Uncharacterized protein OS=Gordonia phage Kiko OX=2500571 GN=42 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84NLVAAAMLALLTLTGCSGEE21 pKa = 4.41TADD24 pKa = 4.63PPAAAVSASSTVAATPSSPDD44 pKa = 2.93TSAASEE50 pKa = 4.53TPAGPPALEE59 pKa = 3.69PVVAQFVLDD68 pKa = 4.0SNASIGDD75 pKa = 3.95DD76 pKa = 3.65YY77 pKa = 11.9SALLSQYY84 pKa = 7.45ATPDD88 pKa = 3.46CASTMLSLGDD98 pKa = 4.09TNTSSPTRR106 pKa = 11.84IVSVQQDD113 pKa = 3.79GTTGTSVTALRR124 pKa = 11.84DD125 pKa = 3.8DD126 pKa = 3.95PTSPGATLQWVYY138 pKa = 11.04SDD140 pKa = 3.16RR141 pKa = 11.84WRR143 pKa = 11.84FTCEE147 pKa = 4.9GIFQQ151 pKa = 3.95

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IL42|A0A3T0IL42_9CAUD Terminase large subunit OS=Gordonia phage Kiko OX=2500571 GN=2 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.71PSAVLTPEE10 pKa = 4.63GLDD13 pKa = 3.59SLITTQEE20 pKa = 3.53AAALCGVSAATIRR33 pKa = 11.84TWANRR38 pKa = 11.84GYY40 pKa = 11.04VDD42 pKa = 3.34TQGVGRR48 pKa = 11.84RR49 pKa = 11.84LEE51 pKa = 4.02PKK53 pKa = 10.33GRR55 pKa = 11.84DD56 pKa = 3.14RR57 pKa = 11.84QGRR60 pKa = 11.84NLYY63 pKa = 10.64LLLDD67 pKa = 3.58VAKK70 pKa = 10.67AEE72 pKa = 3.98NATRR76 pKa = 11.84MRR78 pKa = 11.84RR79 pKa = 11.84ARR81 pKa = 11.84RR82 pKa = 11.84VV83 pKa = 3.01

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14307

47

1629

213.5

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.309 ± 0.539

0.993 ± 0.186

6.864 ± 0.388

5.445 ± 0.319

2.901 ± 0.194

8.436 ± 0.445

1.971 ± 0.2

4.592 ± 0.259

3.006 ± 0.184

8.066 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.096

2.663 ± 0.214

5.997 ± 0.336

3.432 ± 0.172

7.584 ± 0.384

5.459 ± 0.232

6.444 ± 0.239

7.472 ± 0.219

2.048 ± 0.128

2.216 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski