Stenotrophomonas phage YB07

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Menderavirus; unclassified Menderavirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IBG6|A0A482IBG6_9CAUD Uncharacterized protein OS=Stenotrophomonas phage YB07 OX=2555548 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.2IIAKK6 pKa = 9.73DD7 pKa = 3.3QAQADD12 pKa = 4.26TLVKK16 pKa = 10.71AFNLSAADD24 pKa = 3.95LEE26 pKa = 4.55NTLVQNGLEE35 pKa = 4.14IEE37 pKa = 4.9LDD39 pKa = 4.02DD40 pKa = 5.57LDD42 pKa = 5.85LDD44 pKa = 3.91IMIDD48 pKa = 3.39GLYY51 pKa = 8.48YY52 pKa = 10.42AGYY55 pKa = 8.57ASEE58 pKa = 4.33VIEE61 pKa = 4.41VLLEE65 pKa = 3.85ALGSYY70 pKa = 9.78DD71 pKa = 3.55HH72 pKa = 7.27NSILVLL78 pKa = 3.88

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IDW6|A0A482IDW6_9CAUD Uncharacterized protein OS=Stenotrophomonas phage YB07 OX=2555548 PE=4 SV=1
MM1 pKa = 7.55LVTHH5 pKa = 6.68LWILLTFIKK14 pKa = 10.12IIPKK18 pKa = 10.01RR19 pKa = 11.84FLPTPSTCPQYY30 pKa = 10.98LSPHH34 pKa = 5.95SLPSIRR40 pKa = 11.84AASPVSISSFRR51 pKa = 11.84KK52 pKa = 10.04SGFCPNLAVTTSCIIASFSSWNKK75 pKa = 8.77GSRR78 pKa = 11.84IASFIWRR85 pKa = 11.84LDD87 pKa = 3.44SPGVQFSMYY96 pKa = 10.0LSNRR100 pKa = 11.84RR101 pKa = 11.84WYY103 pKa = 10.8SGMKK107 pKa = 10.01SGVKK111 pKa = 10.01SISAMMYY118 pKa = 10.38LDD120 pKa = 4.13FAHH123 pKa = 7.16WW124 pKa = 3.45

Molecular weight: 14.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

257

0

257

47195

38

1931

183.6

20.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.628 ± 0.219

0.994 ± 0.06

6.679 ± 0.134

6.759 ± 0.224

4.255 ± 0.115

6.613 ± 0.179

1.769 ± 0.098

5.975 ± 0.13

6.145 ± 0.204

8.037 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.11

4.751 ± 0.136

4.072 ± 0.143

3.89 ± 0.091

5.2 ± 0.13

5.973 ± 0.159

6.062 ± 0.269

6.746 ± 0.135

1.733 ± 0.08

3.907 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski