Streptococcus satellite phage Javan289
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJ96|A0A4D5ZJ96_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan289 OX=2558610 GN=JavanS289_0003 PE=4 SV=1
MM1 pKa = 7.54 NEE3 pKa = 5.18 LINITLNEE11 pKa = 3.79 NHH13 pKa = 6.57 EE14 pKa = 4.3 PVVIILDD21 pKa = 3.82 EE22 pKa = 4.39 EE23 pKa = 4.81 DD24 pKa = 5.15 DD25 pKa = 4.1 FDD27 pKa = 5.15 FEE29 pKa = 4.31 EE30 pKa = 4.61 TEE32 pKa = 4.09 EE33 pKa = 4.39 EE34 pKa = 3.89 IQAEE38 pKa = 4.37 YY39 pKa = 9.44 EE40 pKa = 4.08 AEE42 pKa = 4.09 YY43 pKa = 10.7 LADD46 pKa = 4.0 DD47 pKa = 4.11 TQDD50 pKa = 3.12 IINWINEE57 pKa = 3.82 SSNAKK62 pKa = 9.06 IFYY65 pKa = 10.31 RR66 pKa = 11.84 ILQQ69 pKa = 3.72
Molecular weight: 8.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.936
IPC2_protein 3.541
IPC_protein 3.439
Toseland 3.274
ProMoST 3.605
Dawson 3.414
Bjellqvist 3.592
Wikipedia 3.338
Rodwell 3.287
Grimsley 3.198
Solomon 3.376
Lehninger 3.325
Nozaki 3.554
DTASelect 3.656
Thurlkill 3.325
EMBOSS 3.35
Sillero 3.554
Patrickios 1.723
IPC_peptide 3.376
IPC2_peptide 3.528
IPC2.peptide.svr19 3.647
Protein with the highest isoelectric point:
>tr|A0A4D5ZI43|A0A4D5ZI43_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan289 OX=2558610 GN=JavanS289_0010 PE=4 SV=1
MM1 pKa = 7.56 FISEE5 pKa = 3.95 NQARR9 pKa = 11.84 AFRR12 pKa = 11.84 HH13 pKa = 5.25 KK14 pKa = 10.21 RR15 pKa = 11.84 ADD17 pKa = 3.23 MMLTKK22 pKa = 10.44 TEE24 pKa = 4.05 LAKK27 pKa = 10.78 KK28 pKa = 10.06 IGVTRR33 pKa = 11.84 ITLSKK38 pKa = 10.11 IEE40 pKa = 4.16 RR41 pKa = 11.84 GNYY44 pKa = 7.93 KK45 pKa = 9.99 APKK48 pKa = 9.57 RR49 pKa = 11.84 IYY51 pKa = 10.03 QSVMDD56 pKa = 5.03 WLTEE60 pKa = 4.03 DD61 pKa = 3.68 LL62 pKa = 4.87
Molecular weight: 7.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.131
IPC2_protein 9.414
IPC_protein 9.502
Toseland 10.365
ProMoST 9.897
Dawson 10.482
Bjellqvist 10.087
Wikipedia 10.599
Rodwell 11.008
Grimsley 10.526
Solomon 10.526
Lehninger 10.511
Nozaki 10.321
DTASelect 10.087
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.379
Patrickios 10.804
IPC_peptide 10.526
IPC2_peptide 8.521
IPC2.peptide.svr19 8.526
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2305
47
520
164.6
19.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.816 ± 0.291
0.954 ± 0.324
5.293 ± 0.491
7.158 ± 0.971
5.944 ± 0.773
4.425 ± 0.566
2.256 ± 0.395
7.679 ± 0.648
8.113 ± 0.894
11.367 ± 0.661
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.296
5.9 ± 0.546
2.993 ± 0.483
3.644 ± 0.297
4.078 ± 0.497
5.597 ± 0.394
5.597 ± 0.606
5.987 ± 0.325
1.475 ± 0.366
4.425 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here