Plasmodium falciparum FCH/4

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania); Plasmodium falciparum

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024VR15|A0A024VR15_PLAFA Uncharacterized protein OS=Plasmodium falciparum FCH/4 OX=1036724 GN=PFFCH_01788 PE=4 SV=1
MM1 pKa = 8.65LEE3 pKa = 3.94EE4 pKa = 4.27RR5 pKa = 11.84NEE7 pKa = 3.97NNEE10 pKa = 3.93NNEE13 pKa = 4.01DD14 pKa = 3.92NEE16 pKa = 4.49NNGYY20 pKa = 8.4NQNNEE25 pKa = 3.51YY26 pKa = 10.56DD27 pKa = 3.53QNNEE31 pKa = 3.35YY32 pKa = 10.64DD33 pKa = 3.51QNNEE37 pKa = 3.35YY38 pKa = 10.64DD39 pKa = 3.51QNNEE43 pKa = 3.35YY44 pKa = 10.64DD45 pKa = 3.51QNNEE49 pKa = 3.35YY50 pKa = 10.64DD51 pKa = 3.51QNNEE55 pKa = 3.37YY56 pKa = 10.72DD57 pKa = 3.43QNNDD61 pKa = 3.01YY62 pKa = 10.88NQNNDD67 pKa = 3.17YY68 pKa = 10.78NQNNDD73 pKa = 3.17YY74 pKa = 10.78NQNNDD79 pKa = 3.17YY80 pKa = 10.78NQNNDD85 pKa = 3.17YY86 pKa = 10.78NQNNDD91 pKa = 3.06YY92 pKa = 10.96NLNSGYY98 pKa = 10.55NLNSGYY104 pKa = 10.36NLNSGYY110 pKa = 10.45SLNSGYY116 pKa = 10.98SLNNVYY122 pKa = 10.32NQYY125 pKa = 11.36SNNIEE130 pKa = 3.87NSGNILNNGFFGSSTNNNNRR150 pKa = 11.84SILGGVRR157 pKa = 11.84EE158 pKa = 4.58PIQLEE163 pKa = 4.0SRR165 pKa = 11.84GFFINGRR172 pKa = 11.84SNNVNRR178 pKa = 11.84ILVNVDD184 pKa = 3.25HH185 pKa = 7.1NNVPINSLNGTNHH198 pKa = 6.96ILGIPNNINMDD209 pKa = 4.0SLIQTILLASSLIINTSARR228 pKa = 11.84AVATSNRR235 pKa = 11.84NTRR238 pKa = 11.84NSNALTSDD246 pKa = 3.54GEE248 pKa = 4.28EE249 pKa = 4.05TMEE252 pKa = 4.89EE253 pKa = 4.03IVDD256 pKa = 4.02DD257 pKa = 4.22GMEE260 pKa = 4.32EE261 pKa = 3.7IDD263 pKa = 4.67LNEE266 pKa = 4.22NSDD269 pKa = 4.44FIHH272 pKa = 7.4DD273 pKa = 4.13GNPLGEE279 pKa = 4.28YY280 pKa = 10.96SNMEE284 pKa = 4.0YY285 pKa = 10.9EE286 pKa = 4.48LLNEE290 pKa = 4.48VSASTDD296 pKa = 3.38FSDD299 pKa = 3.5YY300 pKa = 11.01HH301 pKa = 7.75SVVDD305 pKa = 3.89EE306 pKa = 4.68DD307 pKa = 5.63LINFNDD313 pKa = 3.99DD314 pKa = 3.11VTLEE318 pKa = 4.2TQSMIAHH325 pKa = 6.92GGSLSEE331 pKa = 4.27IEE333 pKa = 4.17EE334 pKa = 4.39TGDD337 pKa = 3.66LSSDD341 pKa = 3.24VDD343 pKa = 3.74RR344 pKa = 11.84LLSSIEE350 pKa = 3.84TTPRR354 pKa = 11.84DD355 pKa = 3.48DD356 pKa = 3.26VVYY359 pKa = 10.56IRR361 pKa = 11.84GDD363 pKa = 3.33IILGEE368 pKa = 4.14DD369 pKa = 3.84EE370 pKa = 5.19IIIEE374 pKa = 4.18NGEE377 pKa = 4.12IILNNTDD384 pKa = 3.64EE385 pKa = 4.7LFEE388 pKa = 5.13DD389 pKa = 4.03VPEE392 pKa = 4.08MLEE395 pKa = 4.01NANLLEE401 pKa = 4.49DD402 pKa = 3.8RR403 pKa = 11.84EE404 pKa = 4.66HH405 pKa = 7.44VFEE408 pKa = 4.42YY409 pKa = 10.54NISVANEE416 pKa = 3.48NTTNQNSYY424 pKa = 10.71NFNAPQDD431 pKa = 4.02NTLTNYY437 pKa = 10.32NISRR441 pKa = 11.84FLQRR445 pKa = 11.84YY446 pKa = 6.69GTTTPNTQHH455 pKa = 6.75NISIHH460 pKa = 6.53RR461 pKa = 11.84DD462 pKa = 3.18GHH464 pKa = 5.2SLRR467 pKa = 11.84SDD469 pKa = 3.19TNIYY473 pKa = 10.86DD474 pKa = 4.61RR475 pKa = 11.84INGPSIRR482 pKa = 11.84EE483 pKa = 3.67NSLYY487 pKa = 10.28PGSFEE492 pKa = 3.77MAQYY496 pKa = 11.03ADD498 pKa = 4.72RR499 pKa = 11.84YY500 pKa = 10.15QMGHH504 pKa = 6.64TIAEE508 pKa = 4.43GFLYY512 pKa = 10.73

Molecular weight: 58.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024VQE8|A0A024VQE8_PLAFA Uncharacterized protein OS=Plasmodium falciparum FCH/4 OX=1036724 GN=PFFCH_01616 PE=4 SV=1
MM1 pKa = 7.34VFPLTLLKK9 pKa = 10.59CSQNQPVMVEE19 pKa = 4.13LKK21 pKa = 10.8NGEE24 pKa = 4.4TYY26 pKa = 10.84SGFLVFCDD34 pKa = 2.98RR35 pKa = 11.84FMNLHH40 pKa = 6.2MKK42 pKa = 10.65NIICTSKK49 pKa = 11.12DD50 pKa = 2.75GDD52 pKa = 4.07KK53 pKa = 10.29FWKK56 pKa = 10.1ISEE59 pKa = 4.46CYY61 pKa = 10.25VRR63 pKa = 11.84GNSIKK68 pKa = 10.42YY69 pKa = 10.05IRR71 pKa = 11.84VQDD74 pKa = 3.68QAIEE78 pKa = 3.9QAIEE82 pKa = 3.64EE83 pKa = 4.28TAEE86 pKa = 3.88QKK88 pKa = 10.3ARR90 pKa = 11.84NMGRR94 pKa = 11.84GRR96 pKa = 11.84GGRR99 pKa = 11.84GRR101 pKa = 11.84GRR103 pKa = 11.84GGMNRR108 pKa = 11.84GTYY111 pKa = 9.58GRR113 pKa = 11.84GRR115 pKa = 11.84RR116 pKa = 11.84GSMRR120 pKa = 11.84RR121 pKa = 11.84GGRR124 pKa = 11.84GQQ126 pKa = 3.02

Molecular weight: 14.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5744

18

5762

3694402

9

10252

641.2

75.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.899 ± 0.022

1.79 ± 0.019

6.389 ± 0.027

6.961 ± 0.041

4.398 ± 0.025

2.766 ± 0.027

2.431 ± 0.015

9.357 ± 0.035

11.684 ± 0.042

7.597 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.014

14.731 ± 0.116

1.931 ± 0.02

2.739 ± 0.016

2.628 ± 0.019

6.396 ± 0.023

4.072 ± 0.019

3.727 ± 0.022

0.489 ± 0.008

5.776 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski