Fulvimarina manganoxydans
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4454 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W2ARJ9|A0A1W2ARJ9_9RHIZ Isochorismatase OS=Fulvimarina manganoxydans OX=937218 GN=SAMN06297251_10533 PE=4 SV=1
MM1 pKa = 7.61 LKK3 pKa = 10.25 QNRR6 pKa = 11.84 TDD8 pKa = 3.45 DD9 pKa = 4.04 SANLRR14 pKa = 11.84 SPSQSQDD21 pKa = 2.49 WAEE24 pKa = 3.69 AAAILEE30 pKa = 4.07 SDD32 pKa = 4.29 EE33 pKa = 5.09 YY34 pKa = 10.89 KK35 pKa = 10.8 ADD37 pKa = 3.31 WGLAGMNASYY47 pKa = 10.6 AYY49 pKa = 10.18 ALGYY53 pKa = 8.68 TGEE56 pKa = 4.5 GIKK59 pKa = 10.59 LGEE62 pKa = 4.26 VDD64 pKa = 3.43 SGIYY68 pKa = 9.42 AAHH71 pKa = 7.38 PEE73 pKa = 4.05 FADD76 pKa = 4.4 GRR78 pKa = 11.84 IHH80 pKa = 7.17 PLDD83 pKa = 3.25 ISGTYY88 pKa = 10.65 LNDD91 pKa = 3.7 GYY93 pKa = 10.91 RR94 pKa = 11.84 YY95 pKa = 10.37 EE96 pKa = 4.11 NLHH99 pKa = 6.05 WEE101 pKa = 3.74 WDD103 pKa = 3.78 GNSWHH108 pKa = 6.58 LVSEE112 pKa = 4.92 GPWDD116 pKa = 3.74 PSRR119 pKa = 11.84 FEE121 pKa = 4.52 ANEE124 pKa = 4.05 SFDD127 pKa = 3.69 TPGVYY132 pKa = 10.16 DD133 pKa = 3.73 GSINGTHH140 pKa = 5.72 GTHH143 pKa = 5.92 VGGSIAAARR152 pKa = 11.84 DD153 pKa = 3.65 GKK155 pKa = 10.94 GMHH158 pKa = 5.87 GVAYY162 pKa = 10.34 LADD165 pKa = 4.52 LFTANTGGTDD175 pKa = 3.51 GMVCGPSEE183 pKa = 4.24 DD184 pKa = 3.73 YY185 pKa = 11.36 NYY187 pKa = 10.66 FKK189 pKa = 10.86 AAYY192 pKa = 9.05 GAVAEE197 pKa = 4.56 AGARR201 pKa = 11.84 VINTSWGQDD210 pKa = 3.04 AASDD214 pKa = 3.96 DD215 pKa = 3.95 FSTLEE220 pKa = 3.9 GLMTVYY226 pKa = 9.41 STHH229 pKa = 6.5 FGKK232 pKa = 8.8 LTWIDD237 pKa = 3.3 AVADD241 pKa = 3.75 VVKK244 pKa = 10.37 EE245 pKa = 3.93 YY246 pKa = 11.71 GEE248 pKa = 4.14 IQTWAAGNNGCFSPVVEE265 pKa = 4.55 ASLPYY270 pKa = 9.79 FRR272 pKa = 11.84 PEE274 pKa = 5.03 LEE276 pKa = 4.36 RR277 pKa = 11.84 NWIAVGASGDD287 pKa = 3.97 TGGHH291 pKa = 5.85 SPDD294 pKa = 3.02 STYY297 pKa = 11.53 AEE299 pKa = 4.14 SWSTKK304 pKa = 10.58 AGVAKK309 pKa = 9.99 YY310 pKa = 6.74 WTVFAPGINIYY321 pKa = 8.96 STVAHH326 pKa = 6.72 HH327 pKa = 7.06 EE328 pKa = 4.74 DD329 pKa = 3.56 GDD331 pKa = 4.06 VWSPEE336 pKa = 3.57 AWGVDD341 pKa = 3.71 PNNPEE346 pKa = 4.0 YY347 pKa = 10.63 TSVSGTSMAAPHH359 pKa = 6.0 ATGALGLVMQRR370 pKa = 11.84 YY371 pKa = 9.2 AYY373 pKa = 10.53 LGNQEE378 pKa = 4.29 ARR380 pKa = 11.84 DD381 pKa = 3.95 VLLTTAQHH389 pKa = 5.67 MDD391 pKa = 3.5 AVEE394 pKa = 4.08 GTPDD398 pKa = 3.72 DD399 pKa = 3.93 TTGLARR405 pKa = 11.84 NDD407 pKa = 3.74 APNEE411 pKa = 3.55 IWGWGAIDD419 pKa = 5.48 LKK421 pKa = 11.21 AAMNGPGQFLGAFTVDD437 pKa = 3.06 MDD439 pKa = 3.92 EE440 pKa = 4.36 TQSDD444 pKa = 3.18 VWYY447 pKa = 8.66 NTITEE452 pKa = 4.11 DD453 pKa = 5.09 ALIQRR458 pKa = 11.84 HH459 pKa = 5.65 RR460 pKa = 11.84 EE461 pKa = 4.21 DD462 pKa = 3.23 IAEE465 pKa = 4.06 QATWQQTLADD475 pKa = 4.42 KK476 pKa = 10.96 GWDD479 pKa = 3.39 EE480 pKa = 4.4 TPPTEE485 pKa = 4.4 ASSIEE490 pKa = 4.06 DD491 pKa = 3.19 QVAYY495 pKa = 10.09 EE496 pKa = 4.2 VGTARR501 pKa = 11.84 AEE503 pKa = 3.8 AAANRR508 pKa = 11.84 VYY510 pKa = 10.46 EE511 pKa = 4.18 GSLIKK516 pKa = 10.51 MGDD519 pKa = 3.13 GTLALMGEE527 pKa = 4.74 GSTYY531 pKa = 10.94 SGGTVLKK538 pKa = 10.77 GGTLEE543 pKa = 4.15 VGALGAAGTGAIEE556 pKa = 4.41 FTDD559 pKa = 4.55 GAQTLRR565 pKa = 11.84 IDD567 pKa = 3.74 HH568 pKa = 6.79 LALSEE573 pKa = 5.01 DD574 pKa = 4.08 EE575 pKa = 4.53 DD576 pKa = 4.07 HH577 pKa = 7.39 SFANLITDD585 pKa = 4.53 FANDD589 pKa = 3.23 GDD591 pKa = 4.24 VIEE594 pKa = 4.39 VCGIGSNVALSFDD607 pKa = 3.93 YY608 pKa = 11.15 ASDD611 pKa = 4.14 LLQLIDD617 pKa = 3.76 TANNVCQATLQLDD630 pKa = 3.65 GDD632 pKa = 4.25 YY633 pKa = 11.19 GGKK636 pKa = 10.26 LFTASYY642 pKa = 10.07 DD643 pKa = 3.68 GEE645 pKa = 4.27 GTVQIEE651 pKa = 4.11 LQAIEE656 pKa = 4.47 ISDD659 pKa = 4.04 ALRR662 pKa = 11.84 SPDD665 pKa = 5.77 NIDD668 pKa = 3.14 LWAQAAEE675 pKa = 4.17 TWEE678 pKa = 4.21 SAEE681 pKa = 4.58 YY682 pKa = 9.7 EE683 pKa = 4.3 ADD685 pKa = 3.05 WGLAGMNASYY695 pKa = 10.6 AYY697 pKa = 10.18 ALGYY701 pKa = 8.68 TGEE704 pKa = 4.5 GIKK707 pKa = 10.59 LGEE710 pKa = 4.26 VDD712 pKa = 3.43 SGIYY716 pKa = 9.42 AAHH719 pKa = 7.34 PEE721 pKa = 3.99 FAGDD725 pKa = 4.16 RR726 pKa = 11.84 IHH728 pKa = 7.22 PLDD731 pKa = 4.27 ISGTYY736 pKa = 10.7 LNDD739 pKa = 4.04 GYY741 pKa = 11.18 SYY743 pKa = 11.48 EE744 pKa = 4.02 EE745 pKa = 4.33 AGWVFNYY752 pKa = 10.47 DD753 pKa = 2.68 EE754 pKa = 5.17 WKK756 pKa = 10.0 WEE758 pKa = 4.32 WIFKK762 pKa = 10.75 GPIEE766 pKa = 4.12 EE767 pKa = 4.5 SRR769 pKa = 11.84 FSAGDD774 pKa = 3.4 EE775 pKa = 4.27 FEE777 pKa = 4.52 TPGVYY782 pKa = 10.35 SNEE785 pKa = 3.95 YY786 pKa = 9.16 NDD788 pKa = 3.4 PHH790 pKa = 5.91 GTHH793 pKa = 6.06 VGGTIAANRR802 pKa = 11.84 DD803 pKa = 3.28 GEE805 pKa = 4.44 GMHH808 pKa = 6.7 GVAFDD813 pKa = 4.49 ANLFLANTHH822 pKa = 5.31 GTDD825 pKa = 3.24 GKK827 pKa = 11.32 VYY829 pKa = 10.37 GANSDD834 pKa = 3.73 YY835 pKa = 11.34 NYY837 pKa = 10.79 FLAAYY842 pKa = 7.44 GANAEE847 pKa = 4.21 AGARR851 pKa = 11.84 AINTSWGTAPGDD863 pKa = 3.43 HH864 pKa = 7.62 DD865 pKa = 4.66 YY866 pKa = 11.78 DD867 pKa = 4.01 SLQGLTDD874 pKa = 4.05 AYY876 pKa = 11.45 SDD878 pKa = 3.71 FFGSLTHH885 pKa = 6.9 LDD887 pKa = 3.02 AMYY890 pKa = 10.61 DD891 pKa = 3.47 VSEE894 pKa = 4.3 NTGVLQVIAAGNDD907 pKa = 3.45 FASNPDD913 pKa = 3.02 IRR915 pKa = 11.84 NSIPYY920 pKa = 8.85 FRR922 pKa = 11.84 PDD924 pKa = 4.66 YY925 pKa = 8.61 EE926 pKa = 4.29 DD927 pKa = 2.97 HH928 pKa = 6.8 WISVGASHH936 pKa = 7.49 RR937 pKa = 11.84 SDD939 pKa = 3.65 EE940 pKa = 4.5 PSPDD944 pKa = 3.19 STGVGFFSDD953 pKa = 3.24 RR954 pKa = 11.84 AGVAKK959 pKa = 10.17 YY960 pKa = 6.83 WTVFAPGLEE969 pKa = 4.36 IYY971 pKa = 10.63 SAVPPSGTDD980 pKa = 3.59 DD981 pKa = 3.26 LWSPEE986 pKa = 3.97 DD987 pKa = 3.49 WSINPDD993 pKa = 3.5 DD994 pKa = 4.84 PNYY997 pKa = 10.03 TSASGTSMAAPHH1009 pKa = 6.0 ATGALGLVMQRR1020 pKa = 11.84 YY1021 pKa = 9.2 AYY1023 pKa = 10.53 LGNQEE1028 pKa = 4.29 ARR1030 pKa = 11.84 DD1031 pKa = 3.95 VLLTTAQHH1039 pKa = 5.67 MDD1041 pKa = 3.5 AVEE1044 pKa = 4.08 GTPDD1048 pKa = 3.72 DD1049 pKa = 3.93 TTGLARR1055 pKa = 11.84 NDD1057 pKa = 3.74 APNEE1061 pKa = 3.55 IWGWGAIDD1069 pKa = 5.48 LKK1071 pKa = 11.21 AAMNGPGQFLGAFTVDD1087 pKa = 3.06 MDD1089 pKa = 3.92 EE1090 pKa = 4.36 TQSDD1094 pKa = 3.18 VWYY1097 pKa = 8.66 NTITEE1102 pKa = 4.11 DD1103 pKa = 5.09 ALIQRR1108 pKa = 11.84 HH1109 pKa = 5.65 RR1110 pKa = 11.84 EE1111 pKa = 4.21 DD1112 pKa = 3.23 IAEE1115 pKa = 4.06 QATWQQTLADD1125 pKa = 4.42 KK1126 pKa = 10.96 GWDD1129 pKa = 3.39 EE1130 pKa = 4.4 TPPTEE1135 pKa = 4.4 ASSIEE1140 pKa = 4.06 DD1141 pKa = 3.19 QVAYY1145 pKa = 10.09 EE1146 pKa = 4.2 VGTARR1151 pKa = 11.84 AEE1153 pKa = 3.8 AAANRR1158 pKa = 11.84 VYY1160 pKa = 10.46 EE1161 pKa = 4.18 GSLIKK1166 pKa = 10.51 MGDD1169 pKa = 3.13 GTLALMGEE1177 pKa = 4.74 GSTYY1181 pKa = 10.94 SGGTVLKK1188 pKa = 10.77 GGTLEE1193 pKa = 4.15 VGALGAAGTGAIEE1206 pKa = 4.41 FTDD1209 pKa = 4.55 GAQTLRR1215 pKa = 11.84 IDD1217 pKa = 3.74 HH1218 pKa = 6.79 LALSEE1223 pKa = 5.01 DD1224 pKa = 4.08 EE1225 pKa = 4.53 DD1226 pKa = 4.07 HH1227 pKa = 7.39 SFANLITDD1235 pKa = 4.53 FANDD1239 pKa = 3.23 GDD1241 pKa = 4.24 VIEE1244 pKa = 4.39 VCGIGSNVALSFDD1257 pKa = 3.93 YY1258 pKa = 11.15 ASDD1261 pKa = 4.14 LLQLIDD1267 pKa = 3.76 TANNVCQATLQLDD1280 pKa = 3.65 GDD1282 pKa = 4.25 YY1283 pKa = 11.19 GGKK1286 pKa = 10.26 LFTASYY1292 pKa = 10.07 DD1293 pKa = 3.68 GEE1295 pKa = 4.27 GTVQIEE1301 pKa = 4.18 LRR1303 pKa = 11.84 VPVYY1307 pKa = 10.72 SGGAVVTDD1315 pKa = 3.52 NGSDD1319 pKa = 3.41 NVEE1322 pKa = 4.14 DD1323 pKa = 4.65 EE1324 pKa = 4.62 IVTGTAGDD1332 pKa = 4.19 DD1333 pKa = 3.74 VFSDD1337 pKa = 4.0 LSFPSNVAGGDD1348 pKa = 3.54 GFDD1351 pKa = 5.52 AIVLPGNITDD1361 pKa = 5.95 FILTPAPGGFTLTKK1375 pKa = 10.51 ASDD1378 pKa = 3.69 SEE1380 pKa = 4.33 TSIFFSDD1387 pKa = 3.42 VEE1389 pKa = 4.56 AIRR1392 pKa = 11.84 FDD1394 pKa = 5.42 DD1395 pKa = 3.54 ITLEE1399 pKa = 4.0 RR1400 pKa = 11.84 HH1401 pKa = 6.74 DD1402 pKa = 4.99 DD1403 pKa = 3.8 PEE1405 pKa = 5.0 AVSLYY1410 pKa = 10.92 SLYY1413 pKa = 9.32 QTVFGRR1419 pKa = 11.84 AAEE1422 pKa = 4.11 INGLSFWFNEE1432 pKa = 4.11 AEE1434 pKa = 4.62 KK1435 pKa = 10.21 GLSMHH1440 pKa = 7.65 DD1441 pKa = 3.11 IATSFAQSDD1450 pKa = 4.13 EE1451 pKa = 4.16 FQTTYY1456 pKa = 11.11 GVDD1459 pKa = 3.7 PSPEE1463 pKa = 4.01 TLVDD1467 pKa = 4.61 GIYY1470 pKa = 11.01 ANIFGRR1476 pKa = 11.84 EE1477 pKa = 3.91 PDD1479 pKa = 3.65 PAGEE1483 pKa = 4.37 AFWLDD1488 pKa = 3.22 AFQNGLEE1495 pKa = 4.24 TADD1498 pKa = 4.0 FLTAFSDD1505 pKa = 3.36 ATEE1508 pKa = 3.91 LHH1510 pKa = 6.04 TLVEE1514 pKa = 4.28 NAVDD1518 pKa = 4.84 DD1519 pKa = 5.25 GIFLIAA1525 pKa = 4.9
Molecular weight: 163.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.872
IPC_protein 3.91
Toseland 3.694
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.024
Patrickios 1.202
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|A0A1W2DWK3|A0A1W2DWK3_9RHIZ Flp pilus assembly protein TadG OS=Fulvimarina manganoxydans OX=937218 GN=SAMN06297251_11842 PE=4 SV=1
MM1 pKa = 7.13 FRR3 pKa = 11.84 SILKK7 pKa = 10.07 ALFVGWVAKK16 pKa = 10.46 KK17 pKa = 7.75 FTGRR21 pKa = 11.84 RR22 pKa = 11.84 AHH24 pKa = 6.8 RR25 pKa = 11.84 RR26 pKa = 11.84 PASQPAPRR34 pKa = 11.84 RR35 pKa = 11.84 FF36 pKa = 3.23
Molecular weight: 4.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4454
0
4454
1381884
25
5642
310.3
33.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.425 ± 0.053
0.784 ± 0.012
6.051 ± 0.032
6.409 ± 0.033
3.83 ± 0.025
8.613 ± 0.038
1.88 ± 0.021
5.293 ± 0.024
3.232 ± 0.03
9.797 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.018
2.449 ± 0.023
5.016 ± 0.029
2.871 ± 0.024
7.227 ± 0.049
5.808 ± 0.031
5.398 ± 0.031
7.146 ± 0.034
1.219 ± 0.016
2.112 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here