Chitinophaga terrae Kim and Jung 2007
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5564 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H4G4B4|A0A1H4G4B4_9BACT Peptidase family M28 OS=Chitinophaga terrae Kim and Jung 2007 OX=408074 GN=SAMN05660909_04939 PE=4 SV=1
MM1 pKa = 7.66 ISFNSEE7 pKa = 3.39 TLDD10 pKa = 3.67 FVNGEE15 pKa = 4.11 TFRR18 pKa = 11.84 PYY20 pKa = 10.46 IKK22 pKa = 10.72 VLFVPAGYY30 pKa = 10.66 SLTVDD35 pKa = 3.99 FNVYY39 pKa = 7.75 EE40 pKa = 4.37 TEE42 pKa = 3.98 NPALFFINTNQYY54 pKa = 11.31 LDD56 pKa = 3.59 IQSATDD62 pKa = 3.17 EE63 pKa = 4.42 DD64 pKa = 4.41 AFLIYY69 pKa = 10.15 YY70 pKa = 10.21 NRR72 pKa = 11.84 DD73 pKa = 3.78 FYY75 pKa = 11.14 CIQIHH80 pKa = 6.26 DD81 pKa = 4.62 EE82 pKa = 4.17 EE83 pKa = 4.85 VACDD87 pKa = 3.95 GLLFNNIFEE96 pKa = 4.38 IPKK99 pKa = 9.92 VDD101 pKa = 4.66 LLEE104 pKa = 5.0 DD105 pKa = 3.44 QLKK108 pKa = 8.29 TVSQLFNQINDD119 pKa = 3.56 EE120 pKa = 4.39 LNFRR124 pKa = 11.84 VAPVLHH130 pKa = 6.76 RR131 pKa = 11.84 SSLTKK136 pKa = 10.62
Molecular weight: 15.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 4.151
IPC_protein 4.088
Toseland 3.897
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.215
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.808
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.368
Thurlkill 3.935
EMBOSS 3.973
Sillero 4.202
Patrickios 1.952
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.095
Protein with the highest isoelectric point:
>tr|A0A1H4BYI5|A0A1H4BYI5_9BACT Dihydrolipoyl dehydrogenase OS=Chitinophaga terrae Kim and Jung 2007 OX=408074 GN=SAMN05660909_02334 PE=3 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.4 FYY4 pKa = 11.02 CSITVRR10 pKa = 11.84 PVLTSSPAIYY20 pKa = 10.0 SIKK23 pKa = 10.37 NNRR26 pKa = 11.84 GNGARR31 pKa = 11.84 KK32 pKa = 9.58 SVMEE36 pKa = 4.14 RR37 pKa = 11.84 HH38 pKa = 5.74 QDD40 pKa = 3.07 HH41 pKa = 6.66 PTIPSHH47 pKa = 7.19 PNNPWLLAALISPATLFTLNFCNKK71 pKa = 9.11 FFRR74 pKa = 5.34
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.677
IPC_protein 10.116
Toseland 10.496
ProMoST 10.452
Dawson 10.628
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 11.008
Grimsley 10.672
Solomon 10.687
Lehninger 10.657
Nozaki 10.511
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.54
Patrickios 10.833
IPC_peptide 10.687
IPC2_peptide 9.399
IPC2.peptide.svr19 8.558
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5564
0
5564
1984624
20
3420
356.7
39.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.01 ± 0.033
0.8 ± 0.01
5.182 ± 0.022
5.518 ± 0.034
4.66 ± 0.025
6.977 ± 0.026
1.871 ± 0.015
6.685 ± 0.026
6.448 ± 0.03
9.6 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.015
5.366 ± 0.032
4.22 ± 0.019
4.037 ± 0.021
4.423 ± 0.022
6.075 ± 0.023
5.791 ± 0.033
6.464 ± 0.027
1.302 ± 0.012
4.217 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here