Sugarcane white streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5CJJ1|X5CJJ1_9GEMI Replication-associated protein OS=Sugarcane white streak virus OX=1492296 PE=3 SV=1
MM1 pKa = 7.12STDD4 pKa = 3.37SSNQGIAAQRR14 pKa = 11.84LSPGLEE20 pKa = 3.98EE21 pKa = 5.47FTTSWPDD28 pKa = 3.11PRR30 pKa = 11.84AGSTSQPRR38 pKa = 11.84VFQFKK43 pKa = 10.01AQNIFLTYY51 pKa = 8.57PRR53 pKa = 11.84CDD55 pKa = 2.95ISVDD59 pKa = 3.38VAARR63 pKa = 11.84TLLTLCHH70 pKa = 6.94RR71 pKa = 11.84FQPLYY76 pKa = 9.76ILCSQEE82 pKa = 3.54HH83 pKa = 6.34HH84 pKa = 7.11ADD86 pKa = 3.66GSNHH90 pKa = 5.74LHH92 pKa = 7.08ILLQTDD98 pKa = 2.66KK99 pKa = 11.23TMYY102 pKa = 9.06TRR104 pKa = 11.84NPHH107 pKa = 5.75YY108 pKa = 10.61FDD110 pKa = 3.64ICGHH114 pKa = 6.5HH115 pKa = 7.23PNIQPAKK122 pKa = 10.16SPDD125 pKa = 3.26NVRR128 pKa = 11.84AYY130 pKa = 9.73ILKK133 pKa = 10.55DD134 pKa = 3.64PITSFEE140 pKa = 4.25EE141 pKa = 5.17GSFQPRR147 pKa = 11.84GSRR150 pKa = 11.84SNTSAIPRR158 pKa = 11.84SGNSGTKK165 pKa = 9.95DD166 pKa = 2.96SLMRR170 pKa = 11.84DD171 pKa = 3.99IINTSTSKK179 pKa = 11.0DD180 pKa = 3.43DD181 pKa = 3.36YY182 pKa = 10.09LTRR185 pKa = 11.84VRR187 pKa = 11.84NTFPFDD193 pKa = 2.74WATRR197 pKa = 11.84LQQFEE202 pKa = 4.32YY203 pKa = 10.29SASKK207 pKa = 10.56LFPEE211 pKa = 4.71PVRR214 pKa = 11.84EE215 pKa = 4.09YY216 pKa = 11.38VNPFPPSEE224 pKa = 4.4PDD226 pKa = 3.33LFCRR230 pKa = 11.84EE231 pKa = 4.09IIDD234 pKa = 3.23RR235 pKa = 11.84WVYY238 pKa = 11.35DD239 pKa = 4.32EE240 pKa = 6.07LDD242 pKa = 3.38MDD244 pKa = 3.53ITDD247 pKa = 4.87AFDD250 pKa = 3.37AAQRR254 pKa = 11.84RR255 pKa = 11.84RR256 pKa = 11.84SLYY259 pKa = 9.46IVGPTRR265 pKa = 11.84TGKK268 pKa = 8.25STWARR273 pKa = 11.84SLGRR277 pKa = 11.84HH278 pKa = 5.9NYY280 pKa = 5.92WQHH283 pKa = 5.35MVDD286 pKa = 4.24FTAYY290 pKa = 7.98DD291 pKa = 3.45TLAKK295 pKa = 10.76YY296 pKa = 10.66NILDD300 pKa = 3.74DD301 pKa = 4.38VPFKK305 pKa = 10.89FCPNWKK311 pKa = 9.47QLVGCQRR318 pKa = 11.84DD319 pKa = 4.19FIVNPKK325 pKa = 7.51YY326 pKa = 10.72AKK328 pKa = 10.08RR329 pKa = 11.84KK330 pKa = 7.85EE331 pKa = 4.07IPGGIPCIILQNPDD345 pKa = 4.37DD346 pKa = 4.28DD347 pKa = 4.39WLPVLSPSQMDD358 pKa = 3.6YY359 pKa = 11.01FVNNCDD365 pKa = 3.53VYY367 pKa = 11.31VMKK370 pKa = 10.46PGEE373 pKa = 4.24KK374 pKa = 10.03FFGGDD379 pKa = 3.28TPVPEE384 pKa = 4.48AQEE387 pKa = 4.21DD388 pKa = 4.45VPDD391 pKa = 4.1GTGSSS396 pKa = 3.61

Molecular weight: 45.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5CZY0|X5CZY0_9GEMI Capsid protein OS=Sugarcane white streak virus OX=1492296 PE=3 SV=1
MM1 pKa = 7.72AGPMRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 8.98PARR11 pKa = 11.84SSRR14 pKa = 11.84RR15 pKa = 11.84IVPWPAEE22 pKa = 3.79ALRR25 pKa = 11.84RR26 pKa = 11.84GYY28 pKa = 8.29TPRR31 pKa = 11.84NSVLRR36 pKa = 11.84GTRR39 pKa = 11.84SGRR42 pKa = 11.84SDD44 pKa = 3.46RR45 pKa = 11.84RR46 pKa = 11.84PSLQVINYY54 pKa = 7.44RR55 pKa = 11.84WSGQSAPIFKK65 pKa = 9.86TGGATYY71 pKa = 10.55LSSTFARR78 pKa = 11.84GNGEE82 pKa = 3.81DD83 pKa = 3.41QRR85 pKa = 11.84RR86 pKa = 11.84GAEE89 pKa = 3.96TIIYY93 pKa = 9.59KK94 pKa = 10.09IAFKK98 pKa = 10.85LVFAVTSSQLKK109 pKa = 8.11YY110 pKa = 10.57VSRR113 pKa = 11.84GMCVLWLVYY122 pKa = 9.95DD123 pKa = 4.3AQPTGIKK130 pKa = 9.87PEE132 pKa = 4.21PKK134 pKa = 10.22DD135 pKa = 3.1IFDD138 pKa = 4.34YY139 pKa = 9.6DD140 pKa = 3.72TGLSQSPMTWSVQRR154 pKa = 11.84ALCNRR159 pKa = 11.84FVVKK163 pKa = 10.42RR164 pKa = 11.84RR165 pKa = 11.84WKK167 pKa = 10.81FEE169 pKa = 3.82LSVNGVSAADD179 pKa = 4.14DD180 pKa = 3.64SSDD183 pKa = 3.34AQLSGKK189 pKa = 7.61TIPYY193 pKa = 9.25SRR195 pKa = 11.84RR196 pKa = 11.84GFDD199 pKa = 3.35KK200 pKa = 10.24FCKK203 pKa = 10.0RR204 pKa = 11.84LATRR208 pKa = 11.84TEE210 pKa = 3.82WKK212 pKa = 8.49NTEE215 pKa = 4.14GGDD218 pKa = 3.86IGDD221 pKa = 3.81IQKK224 pKa = 10.89GALYY228 pKa = 10.58VIAAPGNAMEE238 pKa = 4.8FQAIGNIRR246 pKa = 11.84VYY248 pKa = 10.24FKK250 pKa = 11.13SVGLQQ255 pKa = 3.03

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1012

97

396

253.0

28.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.83 ± 0.708

1.779 ± 0.239

6.719 ± 0.909

3.557 ± 0.255

5.138 ± 0.301

6.126 ± 1.049

2.174 ± 0.676

5.83 ± 0.236

3.854 ± 0.747

6.818 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.779 ± 0.087

3.854 ± 0.363

7.411 ± 0.693

4.447 ± 0.204

7.905 ± 0.82

8.893 ± 0.791

6.423 ± 0.288

5.632 ± 1.064

1.779 ± 0.228

4.051 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski