Pig stool associated circular ssDNA virus GER2011

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; unclassified Smacoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1TE90|I1TE90_9VIRU Uncharacterized protein OS=Pig stool associated circular ssDNA virus GER2011 OX=1114942 PE=4 SV=1
MM1 pKa = 7.46GWFSDD6 pKa = 5.17GIDD9 pKa = 3.46WYY11 pKa = 11.46NKK13 pKa = 9.21YY14 pKa = 10.66INGASNAGWKK24 pKa = 10.4AVMDD28 pKa = 4.55LVGLDD33 pKa = 3.23QADD36 pKa = 3.7ANRR39 pKa = 11.84AQSLLSDD46 pKa = 3.77VPVIGDD52 pKa = 3.47VLQLQQRR59 pKa = 11.84QEE61 pKa = 3.97QISDD65 pKa = 3.54YY66 pKa = 11.14LHH68 pKa = 6.63NKK70 pKa = 9.25DD71 pKa = 4.6MDD73 pKa = 3.67WSDD76 pKa = 3.33VKK78 pKa = 11.28YY79 pKa = 9.69PALIGGGAGRR89 pKa = 11.84SLTSSKK95 pKa = 11.06NFVSKK100 pKa = 10.82NIEE103 pKa = 3.88KK104 pKa = 10.56LYY106 pKa = 10.8RR107 pKa = 11.84DD108 pKa = 3.51

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1TE89|I1TE89_9VIRU Uncharacterized protein OS=Pig stool associated circular ssDNA virus GER2011 OX=1114942 PE=4 SV=1
MM1 pKa = 7.33TSQHH5 pKa = 6.42RR6 pKa = 11.84SEE8 pKa = 4.19RR9 pKa = 11.84WGLLQYY15 pKa = 10.5TPLLCTVSTVFGADD29 pKa = 3.56LSRR32 pKa = 11.84TSSSLGSSTVMSQWLVHH49 pKa = 6.35PCSQLTLSKK58 pKa = 10.88LEE60 pKa = 3.82QRR62 pKa = 11.84LATLHH67 pKa = 6.78LRR69 pKa = 11.84ICLILSCTRR78 pKa = 11.84RR79 pKa = 11.84FPMTRR84 pKa = 11.84STTSSTVSWFTRR96 pKa = 11.84TILLLLTVLPLFLLTVPISGFLRR119 pKa = 11.84HH120 pKa = 5.9RR121 pKa = 11.84RR122 pKa = 11.84ISTSTILYY130 pKa = 10.22SLIRR134 pKa = 11.84MDD136 pKa = 4.63GRR138 pKa = 11.84SPCLRR143 pKa = 11.84LDD145 pKa = 3.74LAA147 pKa = 5.04

Molecular weight: 16.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

929

104

333

185.8

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.167 ± 0.834

1.399 ± 0.59

5.92 ± 1.296

3.767 ± 1.381

4.629 ± 0.617

5.705 ± 0.921

1.615 ± 0.466

5.167 ± 0.431

4.521 ± 0.886

10.118 ± 1.973

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.906 ± 0.812

3.983 ± 0.958

5.167 ± 0.782

4.198 ± 0.569

6.136 ± 1.421

9.473 ± 1.571

7.427 ± 1.806

6.566 ± 0.546

2.045 ± 0.591

4.09 ± 0.977
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski