Murine circovirus
Average proteome isoelectric point is 7.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0SZ83|A0A2S0SZ83_9CIRC ATP-dependent helicase Rep OS=Murine circovirus OX=2171383 PE=3 SV=1
MM1 pKa = 7.51 NEE3 pKa = 3.59 RR4 pKa = 11.84 SITRR8 pKa = 11.84 SLAKK12 pKa = 9.93 RR13 pKa = 11.84 WCGTWNNYY21 pKa = 8.69 GEE23 pKa = 4.26 EE24 pKa = 4.32 EE25 pKa = 4.07 EE26 pKa = 5.34 RR27 pKa = 11.84 LLQRR31 pKa = 11.84 FMEE34 pKa = 4.4 QNCEE38 pKa = 3.75 YY39 pKa = 10.81 ACYY42 pKa = 10.1 GKK44 pKa = 9.98 EE45 pKa = 3.86 QGVQGTPHH53 pKa = 6.06 LQIYY57 pKa = 8.67 MRR59 pKa = 11.84 FAKK62 pKa = 9.95 KK63 pKa = 10.2 CRR65 pKa = 11.84 GSFIKK70 pKa = 10.46 RR71 pKa = 11.84 ALPKK75 pKa = 10.12 IHH77 pKa = 6.99 FEE79 pKa = 4.13 VCKK82 pKa = 10.03 GTEE85 pKa = 3.97 LQNIQYY91 pKa = 10.18 CSKK94 pKa = 9.97 EE95 pKa = 3.81 NKK97 pKa = 9.71 AFEE100 pKa = 4.21 IGEE103 pKa = 4.12 PRR105 pKa = 11.84 NEE107 pKa = 4.09 VCKK110 pKa = 10.58 EE111 pKa = 3.56 RR112 pKa = 11.84 AKK114 pKa = 10.36 VARR117 pKa = 11.84 TRR119 pKa = 11.84 EE120 pKa = 4.06 IIDD123 pKa = 3.89 AYY125 pKa = 9.09 MTMDD129 pKa = 3.4 YY130 pKa = 11.19 LRR132 pKa = 11.84 FRR134 pKa = 11.84 EE135 pKa = 4.27 KK136 pKa = 11.15 YY137 pKa = 8.39 PWEE140 pKa = 4.14 ALNMKK145 pKa = 10.01 KK146 pKa = 10.24 KK147 pKa = 10.36 LEE149 pKa = 3.88 EE150 pKa = 3.96 WRR152 pKa = 11.84 IDD154 pKa = 3.25 TLMVKK159 pKa = 9.65 EE160 pKa = 4.36 PWPGEE165 pKa = 3.98 LKK167 pKa = 10.83 DD168 pKa = 3.55 KK169 pKa = 11.1 NFWVWGPPGTGKK181 pKa = 10.42 SRR183 pKa = 11.84 WARR186 pKa = 11.84 SQCDD190 pKa = 3.11 PTMIYY195 pKa = 10.66 LKK197 pKa = 10.57 AANKK201 pKa = 7.83 WWNGYY206 pKa = 9.25 IDD208 pKa = 4.35 NKK210 pKa = 10.39 HH211 pKa = 6.16 KK212 pKa = 10.89 LVLFEE217 pKa = 5.96 DD218 pKa = 4.88 FPKK221 pKa = 10.65 DD222 pKa = 3.6 AKK224 pKa = 10.91 FLGALMKK231 pKa = 10.32 VWADD235 pKa = 3.27 RR236 pKa = 11.84 YY237 pKa = 8.98 TFTAEE242 pKa = 3.8 IKK244 pKa = 10.4 GGAIFIEE251 pKa = 4.37 PSNWALIVTANYY263 pKa = 9.99 SIQEE267 pKa = 4.39 CFDD270 pKa = 3.68 PTDD273 pKa = 3.35 VEE275 pKa = 3.98 ALRR278 pKa = 11.84 RR279 pKa = 11.84 RR280 pKa = 11.84 FTEE283 pKa = 3.38 IRR285 pKa = 11.84 FEE287 pKa = 4.08 EE288 pKa = 4.52 EE289 pKa = 3.23 EE290 pKa = 5.88 DD291 pKa = 3.27 IIQYY295 pKa = 8.62 TKK297 pKa = 10.64 IKK299 pKa = 10.4 FFEE302 pKa = 4.11
Molecular weight: 36.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.405
IPC2_protein 7.79
IPC_protein 7.644
Toseland 7.234
ProMoST 8.185
Dawson 8.39
Bjellqvist 8.697
Wikipedia 8.317
Rodwell 8.419
Grimsley 7.19
Solomon 8.478
Lehninger 8.478
Nozaki 8.916
DTASelect 8.434
Thurlkill 8.507
EMBOSS 8.595
Sillero 8.829
Patrickios 4.469
IPC_peptide 8.463
IPC2_peptide 7.541
IPC2.peptide.svr19 7.841
Protein with the highest isoelectric point:
>tr|A0A2S0SZ83|A0A2S0SZ83_9CIRC ATP-dependent helicase Rep OS=Murine circovirus OX=2171383 PE=3 SV=1
MM1 pKa = 7.51 NEE3 pKa = 3.59 RR4 pKa = 11.84 SITRR8 pKa = 11.84 SLAKK12 pKa = 9.93 RR13 pKa = 11.84 WCGTWNNYY21 pKa = 8.69 GEE23 pKa = 4.26 EE24 pKa = 4.32 EE25 pKa = 4.07 EE26 pKa = 5.34 RR27 pKa = 11.84 LLQRR31 pKa = 11.84 FMEE34 pKa = 4.4 QNCEE38 pKa = 3.75 YY39 pKa = 10.81 ACYY42 pKa = 10.1 GKK44 pKa = 9.98 EE45 pKa = 3.86 QGVQGTPHH53 pKa = 6.06 LQIYY57 pKa = 8.67 MRR59 pKa = 11.84 FAKK62 pKa = 9.95 KK63 pKa = 10.2 CRR65 pKa = 11.84 GSFIKK70 pKa = 10.46 RR71 pKa = 11.84 ALPKK75 pKa = 10.12 IHH77 pKa = 6.99 FEE79 pKa = 4.13 VCKK82 pKa = 10.03 GTEE85 pKa = 3.97 LQNIQYY91 pKa = 10.18 CSKK94 pKa = 9.97 EE95 pKa = 3.81 NKK97 pKa = 9.71 AFEE100 pKa = 4.21 IGEE103 pKa = 4.12 PRR105 pKa = 11.84 NEE107 pKa = 4.09 VCKK110 pKa = 10.58 EE111 pKa = 3.56 RR112 pKa = 11.84 AKK114 pKa = 10.36 VARR117 pKa = 11.84 TRR119 pKa = 11.84 EE120 pKa = 4.06 IIDD123 pKa = 3.89 AYY125 pKa = 9.09 MTMDD129 pKa = 3.4 YY130 pKa = 11.19 LRR132 pKa = 11.84 FRR134 pKa = 11.84 EE135 pKa = 4.27 KK136 pKa = 11.15 YY137 pKa = 8.39 PWEE140 pKa = 4.14 ALNMKK145 pKa = 10.01 KK146 pKa = 10.24 KK147 pKa = 10.36 LEE149 pKa = 3.88 EE150 pKa = 3.96 WRR152 pKa = 11.84 IDD154 pKa = 3.25 TLMVKK159 pKa = 9.65 EE160 pKa = 4.36 PWPGEE165 pKa = 3.98 LKK167 pKa = 10.83 DD168 pKa = 3.55 KK169 pKa = 11.1 NFWVWGPPGTGKK181 pKa = 10.42 SRR183 pKa = 11.84 WARR186 pKa = 11.84 SQCDD190 pKa = 3.11 PTMIYY195 pKa = 10.66 LKK197 pKa = 10.57 AANKK201 pKa = 7.83 WWNGYY206 pKa = 9.25 IDD208 pKa = 4.35 NKK210 pKa = 10.39 HH211 pKa = 6.16 KK212 pKa = 10.89 LVLFEE217 pKa = 5.96 DD218 pKa = 4.88 FPKK221 pKa = 10.65 DD222 pKa = 3.6 AKK224 pKa = 10.91 FLGALMKK231 pKa = 10.32 VWADD235 pKa = 3.27 RR236 pKa = 11.84 YY237 pKa = 8.98 TFTAEE242 pKa = 3.8 IKK244 pKa = 10.4 GGAIFIEE251 pKa = 4.37 PSNWALIVTANYY263 pKa = 9.99 SIQEE267 pKa = 4.39 CFDD270 pKa = 3.68 PTDD273 pKa = 3.35 VEE275 pKa = 3.98 ALRR278 pKa = 11.84 RR279 pKa = 11.84 RR280 pKa = 11.84 FTEE283 pKa = 3.38 IRR285 pKa = 11.84 FEE287 pKa = 4.08 EE288 pKa = 4.52 EE289 pKa = 3.23 EE290 pKa = 5.88 DD291 pKa = 3.27 IIQYY295 pKa = 8.62 TKK297 pKa = 10.64 IKK299 pKa = 10.4 FFEE302 pKa = 4.11
Molecular weight: 36.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.405
IPC2_protein 7.79
IPC_protein 7.644
Toseland 7.234
ProMoST 8.185
Dawson 8.39
Bjellqvist 8.697
Wikipedia 8.317
Rodwell 8.419
Grimsley 7.19
Solomon 8.478
Lehninger 8.478
Nozaki 8.916
DTASelect 8.434
Thurlkill 8.507
EMBOSS 8.595
Sillero 8.829
Patrickios 4.469
IPC_peptide 8.463
IPC2_peptide 7.541
IPC2.peptide.svr19 7.841
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
302
302
302
302.0
36.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.623 ± 0.0
2.98 ± 0.0
3.974 ± 0.0
10.927 ± 0.0
5.629 ± 0.0
5.298 ± 0.0
0.993 ± 0.0
6.623 ± 0.0
9.603 ± 0.0
5.96 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.98 ± 0.0
4.636 ± 0.0
3.974 ± 0.0
3.311 ± 0.0
7.285 ± 0.0
2.649 ± 0.0
4.967 ± 0.0
3.311 ± 0.0
3.974 ± 0.0
4.305 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here