Streptococcus satellite phage Javan225
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHR0|A0A4D5ZHR0_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan225 OX=2558580 GN=JavanS225_0001 PE=3 SV=1
MM1 pKa = 7.1 KK2 pKa = 10.41 HH3 pKa = 6.2 EE4 pKa = 4.54 EE5 pKa = 4.04 LTLTEE10 pKa = 4.31 LKK12 pKa = 10.64 DD13 pKa = 3.32 WTKK16 pKa = 10.5 SARR19 pKa = 11.84 NLVEE23 pKa = 4.73 HH24 pKa = 6.5 LQDD27 pKa = 5.13 DD28 pKa = 4.2 YY29 pKa = 11.95 HH30 pKa = 7.94 DD31 pKa = 4.23 YY32 pKa = 11.44 LPTGKK37 pKa = 10.28 AEE39 pKa = 4.26 AFGSITNSIYY49 pKa = 11.19 NCLDD53 pKa = 3.3 CLAEE57 pKa = 4.17 MFDD60 pKa = 3.73 NDD62 pKa = 3.49 EE63 pKa = 4.65 LEE65 pKa = 4.24 LVEE68 pKa = 4.15 YY69 pKa = 9.87 TIDD72 pKa = 3.83 PQEE75 pKa = 3.93 TTIDD79 pKa = 3.77 DD80 pKa = 3.9 NQADD84 pKa = 4.66 EE85 pKa = 4.0 IAQYY89 pKa = 10.89 NEE91 pKa = 3.57 LMKK94 pKa = 10.63 EE95 pKa = 4.13 VEE97 pKa = 4.64 DD98 pKa = 3.69 SDD100 pKa = 4.11 KK101 pKa = 10.52 EE102 pKa = 3.93 ARR104 pKa = 11.84 EE105 pKa = 4.15 IVCYY109 pKa = 10.15 EE110 pKa = 3.89 YY111 pKa = 11.07 DD112 pKa = 3.84 DD113 pKa = 5.08 TIHH116 pKa = 6.98 EE117 pKa = 4.72 YY118 pKa = 9.73 IDD120 pKa = 3.8 KK121 pKa = 9.55 RR122 pKa = 11.84 TEE124 pKa = 3.8 QLKK127 pKa = 9.5 EE128 pKa = 3.5 QATFEE133 pKa = 4.0 EE134 pKa = 4.62 LAYY137 pKa = 9.82 KK138 pKa = 9.5 VALYY142 pKa = 10.32 EE143 pKa = 5.07 SEE145 pKa = 4.11 LLDD148 pKa = 3.57 YY149 pKa = 11.28 AEE151 pKa = 5.23 RR152 pKa = 11.84 LLSDD156 pKa = 3.74 EE157 pKa = 4.79 PLNADD162 pKa = 3.49 SEE164 pKa = 4.84 TAIGTLDD171 pKa = 3.63 MLDD174 pKa = 4.84 DD175 pKa = 3.92 EE176 pKa = 6.32 AIDD179 pKa = 3.89 LFKK182 pKa = 11.28 SVDD185 pKa = 3.22 VDD187 pKa = 3.55 NEE189 pKa = 4.36 YY190 pKa = 11.0 QGLEE194 pKa = 4.14 YY195 pKa = 11.23 YY196 pKa = 8.19 NTSLNKK202 pKa = 9.95 EE203 pKa = 4.21 DD204 pKa = 3.57
Molecular weight: 23.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.643
ProMoST 3.948
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.948
Patrickios 1.265
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A4D5ZHS1|A0A4D5ZHS1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan225 OX=2558580 GN=JavanS225_0011 PE=4 SV=1
MM1 pKa = 7.51 NIKK4 pKa = 10.04 EE5 pKa = 4.29 VIKK8 pKa = 10.83 KK9 pKa = 10.43 DD10 pKa = 3.21 GTKK13 pKa = 9.85 VYY15 pKa = 10.42 RR16 pKa = 11.84 SNVYY20 pKa = 10.53 LGVDD24 pKa = 4.04 SITGKK29 pKa = 10.18 KK30 pKa = 10.14 VKK32 pKa = 8.5 TTVTGRR38 pKa = 11.84 TKK40 pKa = 10.89 KK41 pKa = 9.66 EE42 pKa = 4.03 VKK44 pKa = 9.46 TKK46 pKa = 10.56 AQQAQSNFKK55 pKa = 11.0 ANGSTVFKK63 pKa = 10.15 RR64 pKa = 11.84 VEE66 pKa = 3.73 VTTYY70 pKa = 11.13 KK71 pKa = 10.76 EE72 pKa = 4.39 LTDD75 pKa = 3.82 LWLEE79 pKa = 4.13 NYY81 pKa = 10.11 QMTVKK86 pKa = 10.31 PQTLVNTRR94 pKa = 11.84 QFLRR98 pKa = 11.84 NHH100 pKa = 6.87 ILPVFGDD107 pKa = 3.56 MQLDD111 pKa = 4.24 KK112 pKa = 11.2 IHH114 pKa = 6.9 IAHH117 pKa = 6.2 IQSWVNKK124 pKa = 9.54 LAFKK128 pKa = 10.19 IVNYY132 pKa = 10.05 GVAASINKK140 pKa = 9.97 RR141 pKa = 11.84 ILQYY145 pKa = 10.53 GVSMQLIPFNPARR158 pKa = 11.84 EE159 pKa = 4.31 VILPRR164 pKa = 11.84 PQKK167 pKa = 10.82 AGANRR172 pKa = 11.84 IKK174 pKa = 10.79 FIDD177 pKa = 3.91 KK178 pKa = 10.6 EE179 pKa = 4.13 DD180 pKa = 3.62 LKK182 pKa = 10.6 TFLDD186 pKa = 3.69 YY187 pKa = 10.59 MEE189 pKa = 5.45 RR190 pKa = 11.84 LAPTAYY196 pKa = 10.16 NYY198 pKa = 11.09 YY199 pKa = 9.71 YY200 pKa = 11.02 DD201 pKa = 3.73 SVLYY205 pKa = 10.49 KK206 pKa = 10.87 LLLATGCRR214 pKa = 11.84 YY215 pKa = 10.48 GEE217 pKa = 4.05 AVALEE222 pKa = 4.14 WSDD225 pKa = 3.78 IDD227 pKa = 5.0 FDD229 pKa = 3.99 NATINITKK237 pKa = 9.12 TYY239 pKa = 9.96 NRR241 pKa = 11.84 IVKK244 pKa = 9.79 QVGTPKK250 pKa = 10.27 SKK252 pKa = 10.81 AGIRR256 pKa = 11.84 IISIDD261 pKa = 3.43 NKK263 pKa = 9.44 TILMLKK269 pKa = 9.54 QYY271 pKa = 10.83 RR272 pKa = 11.84 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QAFMEE281 pKa = 4.42 IGAPAPALVFSTTISQYY298 pKa = 9.95 PNSDD302 pKa = 2.89 ARR304 pKa = 11.84 TKK306 pKa = 10.38 SLRR309 pKa = 11.84 HH310 pKa = 5.31 RR311 pKa = 11.84 CKK313 pKa = 10.41 EE314 pKa = 3.57 AGIPQFTFHH323 pKa = 7.42 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGIGYY340 pKa = 9.74 KK341 pKa = 9.96 EE342 pKa = 3.9 LQHH345 pKa = 6.67 RR346 pKa = 11.84 LGHH349 pKa = 6.08 ATLAMTMDD357 pKa = 4.61 TYY359 pKa = 11.85 SHH361 pKa = 7.05 LSKK364 pKa = 10.62 EE365 pKa = 4.29 KK366 pKa = 9.9 EE367 pKa = 4.1 KK368 pKa = 11.05 EE369 pKa = 3.73 AVLYY373 pKa = 10.0 YY374 pKa = 10.87 GKK376 pKa = 10.6 ALQNLL381 pKa = 3.91
Molecular weight: 43.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.116
IPC2_protein 9.385
IPC_protein 9.311
Toseland 9.999
ProMoST 9.677
Dawson 10.204
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.701
Grimsley 10.277
Solomon 10.218
Lehninger 10.189
Nozaki 9.984
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.116
Patrickios 10.16
IPC_peptide 10.218
IPC2_peptide 8.39
IPC2.peptide.svr19 8.389
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2236
37
517
159.7
18.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.68 ± 0.818
0.85 ± 0.151
6.172 ± 0.79
8.274 ± 0.994
4.562 ± 0.458
4.517 ± 0.557
1.565 ± 0.229
7.245 ± 0.431
8.855 ± 0.677
10.018 ± 0.689
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.549 ± 0.299
6.574 ± 0.51
3.354 ± 0.496
4.204 ± 0.44
4.07 ± 0.514
4.293 ± 0.327
5.814 ± 0.473
5.367 ± 0.431
1.029 ± 0.219
5.009 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here