Burkholderia sp. DHOD12

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8IH53|A0A4P8IH53_9BURK Peptidase_S9 domain-containing protein OS=Burkholderia sp. DHOD12 OX=2571746 GN=FAZ95_01725 PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 4.9NDD4 pKa = 3.4YY5 pKa = 11.59DD6 pKa = 3.87EE7 pKa = 5.13VNIYY11 pKa = 10.39SEE13 pKa = 4.56EE14 pKa = 4.28PPVCPYY20 pKa = 10.69CDD22 pKa = 3.49EE23 pKa = 5.42LGSCPHH29 pKa = 6.71LLLNVEE35 pKa = 4.19VTFRR39 pKa = 11.84TVEE42 pKa = 3.97SGVLFDD48 pKa = 5.06AFNARR53 pKa = 11.84LLSAAEE59 pKa = 3.98QNGGNPSCDD68 pKa = 2.97GSDD71 pKa = 4.27RR72 pKa = 11.84FDD74 pKa = 3.43QLLSEE79 pKa = 4.27VDD81 pKa = 3.68EE82 pKa = 4.77LADD85 pKa = 3.84AVRR88 pKa = 11.84YY89 pKa = 6.62WTFEE93 pKa = 4.37DD94 pKa = 4.74GPGMSSNFQLYY105 pKa = 9.01YY106 pKa = 10.75AEE108 pKa = 4.43SPEE111 pKa = 4.02RR112 pKa = 11.84TMKK115 pKa = 10.81ALEE118 pKa = 4.33RR119 pKa = 11.84LSAPII124 pKa = 4.1

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8IRB3|A0A4P8IRB3_9BURK Lon protease OS=Burkholderia sp. DHOD12 OX=2571746 GN=lon PE=2 SV=1
MM1 pKa = 7.17HH2 pKa = 7.11TRR4 pKa = 11.84LTTGIKK10 pKa = 9.81RR11 pKa = 11.84VALRR15 pKa = 11.84AARR18 pKa = 11.84PARR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 4.44IVRR26 pKa = 11.84ALRR29 pKa = 11.84HH30 pKa = 4.81HH31 pKa = 7.29AARR34 pKa = 11.84TRR36 pKa = 11.84QLAQQRR42 pKa = 11.84HH43 pKa = 4.76ATSADD48 pKa = 3.18GLRR51 pKa = 11.84AWFQTIVSMLRR62 pKa = 11.84VRR64 pKa = 11.84AGARR68 pKa = 11.84PFSRR72 pKa = 11.84FSLRR76 pKa = 11.84TLLPKK81 pKa = 10.49PPVVLRR87 pKa = 11.84ALTSRR92 pKa = 11.84RR93 pKa = 11.84TPDD96 pKa = 2.76VSLRR100 pKa = 11.84GASRR104 pKa = 11.84RR105 pKa = 11.84PRR107 pKa = 11.84RR108 pKa = 11.84LAASTGWFAFAARR121 pKa = 4.17

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7436

0

7436

2401986

20

5375

323.0

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.696 ± 0.043

0.966 ± 0.008

5.363 ± 0.02

5.235 ± 0.031

3.708 ± 0.023

8.125 ± 0.032

2.304 ± 0.014

4.77 ± 0.019

3.148 ± 0.025

10.156 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.015

2.872 ± 0.024

5.041 ± 0.024

3.566 ± 0.019

6.783 ± 0.032

5.899 ± 0.06

5.488 ± 0.045

7.745 ± 0.028

1.364 ± 0.012

2.456 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski