Paenibacillus sp. Root444D2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7JNV2|A0A0Q7JNV2_9BACL Phosphoglycerate kinase OS=Paenibacillus sp. Root444D2 OX=1736538 GN=pgk PE=3 SV=1
MM1 pKa = 7.17QNIPALSQLPEE12 pKa = 4.26LPNGCEE18 pKa = 3.76AVVATMLLNWAGVPVSKK35 pKa = 11.09EE36 pKa = 3.66EE37 pKa = 3.94VAAALPLGEE46 pKa = 4.79LPYY49 pKa = 11.24VNDD52 pKa = 4.27DD53 pKa = 3.64GAFIGSNPKK62 pKa = 10.36DD63 pKa = 3.46VFVGNPYY70 pKa = 9.99EE71 pKa = 4.05VGYY74 pKa = 10.63GIYY77 pKa = 9.79HH78 pKa = 6.77KK79 pKa = 10.25PIADD83 pKa = 4.73MLDD86 pKa = 2.65QWLPGRR92 pKa = 11.84VKK94 pKa = 10.51DD95 pKa = 3.74LSGIAFEE102 pKa = 5.64DD103 pKa = 3.94LLTTIVQGKK112 pKa = 8.09PAMIWATEE120 pKa = 4.01HH121 pKa = 6.57MDD123 pKa = 3.5TPYY126 pKa = 11.38LEE128 pKa = 5.25MEE130 pKa = 4.15WQDD133 pKa = 3.45EE134 pKa = 4.23EE135 pKa = 4.63GQLVEE140 pKa = 4.84WYY142 pKa = 10.06QPEE145 pKa = 4.16HH146 pKa = 6.64ALLLTGWDD154 pKa = 3.32DD155 pKa = 4.71DD156 pKa = 4.12YY157 pKa = 11.98AYY159 pKa = 10.5MNDD162 pKa = 3.52PMTGKK167 pKa = 9.93QEE169 pKa = 4.06SYY171 pKa = 10.61AISDD175 pKa = 4.25FKK177 pKa = 10.52TVWEE181 pKa = 4.14QMGSQAITVNN191 pKa = 3.49

Molecular weight: 21.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7JJ07|A0A0Q7JJ07_9BACL GGDEF domain-containing protein OS=Paenibacillus sp. Root444D2 OX=1736538 GN=ASD40_02085 PE=4 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6948

0

6948

2269465

26

2316

326.6

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.63 ± 0.034

0.756 ± 0.009

5.066 ± 0.024

6.378 ± 0.033

4.379 ± 0.023

7.096 ± 0.028

2.181 ± 0.014

7.2 ± 0.03

5.756 ± 0.026

10.07 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.013

4.105 ± 0.02

3.981 ± 0.019

4.009 ± 0.021

4.528 ± 0.023

6.49 ± 0.025

5.605 ± 0.026

7.023 ± 0.022

1.345 ± 0.013

3.546 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski