Bacillus sp. FJAT-27264

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5758 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8W5E3|A0A1B8W5E3_9BACI Phenylalanine--tRNA ligase beta subunit OS=Bacillus sp. FJAT-27264 OX=1850362 GN=pheT PE=3 SV=1
MM1 pKa = 7.23EE2 pKa = 4.22TTVCPWCDD10 pKa = 3.33TEE12 pKa = 4.97IVWDD16 pKa = 3.93EE17 pKa = 3.94EE18 pKa = 4.31FGPEE22 pKa = 4.68EE23 pKa = 4.15YY24 pKa = 10.42CPHH27 pKa = 6.94CNNEE31 pKa = 3.9LKK33 pKa = 10.47GYY35 pKa = 8.16RR36 pKa = 11.84TVNVTSDD43 pKa = 3.61DD44 pKa = 4.27LEE46 pKa = 6.58DD47 pKa = 3.74EE48 pKa = 4.98LEE50 pKa = 4.35VKK52 pKa = 10.44EE53 pKa = 4.34DD54 pKa = 3.57TDD56 pKa = 4.31EE57 pKa = 4.71EE58 pKa = 4.66SLNDD62 pKa = 3.97DD63 pKa = 4.9DD64 pKa = 6.5LWDD67 pKa = 4.54DD68 pKa = 3.88SEE70 pKa = 5.51KK71 pKa = 11.34DD72 pKa = 3.43GVVPIFNTLEE82 pKa = 4.01QFRR85 pKa = 11.84DD86 pKa = 3.83DD87 pKa = 4.6YY88 pKa = 11.87DD89 pKa = 3.74LQKK92 pKa = 11.12YY93 pKa = 6.8EE94 pKa = 4.21QNSSAILSQQEE105 pKa = 4.05EE106 pKa = 4.53APEE109 pKa = 4.38CPQCHH114 pKa = 6.11EE115 pKa = 4.62LLLLAGTQEE124 pKa = 4.32VNQFDD129 pKa = 4.15PSSPAVLGGPVLKK142 pKa = 10.76APFSLNVFVCPSCFHH157 pKa = 5.87VQHH160 pKa = 6.69NLAQKK165 pKa = 10.78DD166 pKa = 3.57RR167 pKa = 11.84VQLVRR172 pKa = 11.84NLSIGNN178 pKa = 3.72

Molecular weight: 20.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8VWM0|A0A1B8VWM0_9BACI DNA polymerase III OS=Bacillus sp. FJAT-27264 OX=1850362 GN=A8L34_21745 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5758

0

5758

1853569

26

4657

321.9

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.068 ± 0.037

0.697 ± 0.009

5.036 ± 0.024

6.7 ± 0.045

4.093 ± 0.023

7.536 ± 0.032

2.031 ± 0.016

6.763 ± 0.032

5.369 ± 0.03

10.417 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.017

3.971 ± 0.024

4.012 ± 0.02

3.822 ± 0.022

4.799 ± 0.029

6.641 ± 0.027

5.571 ± 0.036

7.063 ± 0.028

1.235 ± 0.013

3.494 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski