Amino acid dipepetide frequency for Methylosinus sp. 3S-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.884AlaAla: 21.884 ± 0.217	1.24AlaCys: 1.24 ± 0.035	7.194AlaAsp: 7.194 ± 0.088	8.801AlaGlu: 8.801 ± 0.119	5.023AlaPhe: 5.023 ± 0.078	11.071AlaGly: 11.071 ± 0.129	2.678AlaHis: 2.678 ± 0.05	6.528AlaIle: 6.528 ± 0.07	4.128AlaLys: 4.128 ± 0.075	16.386AlaLeu: 16.386 ± 0.162	2.968AlaMet: 2.968 ± 0.06	2.767AlaAsn: 2.767 ± 0.047	8.162AlaPro: 8.162 ± 0.114	4.326AlaGln: 4.326 ± 0.058	11.744AlaArg: 11.744 ± 0.139	7.523AlaSer: 7.523 ± 0.095	6.685AlaThr: 6.685 ± 0.082	8.383AlaVal: 8.383 ± 0.091	1.348AlaTrp: 1.348 ± 0.033	2.621AlaTyr: 2.621 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.353CysAla: 1.353 ± 0.032	0.15CysCys: 0.15 ± 0.013	0.572CysAsp: 0.572 ± 0.022	0.506CysGlu: 0.506 ± 0.019	0.341CysPhe: 0.341 ± 0.018	0.972CysGly: 0.972 ± 0.036	0.196CysHis: 0.196 ± 0.014	0.291CysIle: 0.291 ± 0.016	0.216CysLys: 0.216 ± 0.014	0.746CysLeu: 0.746 ± 0.027	0.135CysMet: 0.135 ± 0.009	0.193CysAsn: 0.193 ± 0.012	0.479CysPro: 0.479 ± 0.022	0.167CysGln: 0.167 ± 0.011	0.712CysArg: 0.712 ± 0.025	0.485CysSer: 0.485 ± 0.019	0.321CysThr: 0.321 ± 0.017	0.761CysVal: 0.761 ± 0.029	0.171CysTrp: 0.171 ± 0.017	0.208CysTyr: 0.208 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.139AspAla: 8.139 ± 0.098	0.575AspCys: 0.575 ± 0.022	3.184AspAsp: 3.184 ± 0.055	3.697AspGlu: 3.697 ± 0.063	2.254AspPhe: 2.254 ± 0.036	5.041AspGly: 5.041 ± 0.078	1.244AspHis: 1.244 ± 0.032	2.908AspIle: 2.908 ± 0.048	1.611AspLys: 1.611 ± 0.038	5.927AspLeu: 5.927 ± 0.07	1.089AspMet: 1.089 ± 0.025	1.202AspAsn: 1.202 ± 0.034	3.469AspPro: 3.469 ± 0.051	1.274AspGln: 1.274 ± 0.03	4.24AspArg: 4.24 ± 0.065	2.302AspSer: 2.302 ± 0.039	1.992AspThr: 1.992 ± 0.045	4.009AspVal: 4.009 ± 0.064	0.947AspTrp: 0.947 ± 0.032	1.543AspTyr: 1.543 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	8.922GluAla: 8.922 ± 0.118	0.385GluCys: 0.385 ± 0.02	2.694GluAsp: 2.694 ± 0.055	3.428GluGlu: 3.428 ± 0.066	1.769GluPhe: 1.769 ± 0.041	4.167GluGly: 4.167 ± 0.061	1.159GluHis: 1.159 ± 0.034	3.678GluIle: 3.678 ± 0.055	2.277GluLys: 2.277 ± 0.049	5.653GluLeu: 5.653 ± 0.078	1.302GluMet: 1.302 ± 0.031	1.448GluAsn: 1.448 ± 0.033	3.182GluPro: 3.182 ± 0.058	2.041GluGln: 2.041 ± 0.042	6.227GluArg: 6.227 ± 0.088	2.777GluSer: 2.777 ± 0.052	3.89GluThr: 3.89 ± 0.057	3.317GluVal: 3.317 ± 0.056	0.725GluTrp: 0.725 ± 0.024	0.805GluTyr: 0.805 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	5.141PheAla: 5.141 ± 0.062	0.46PheCys: 0.46 ± 0.021	2.979PheAsp: 2.979 ± 0.048	2.301PheGlu: 2.301 ± 0.051	1.527PhePhe: 1.527 ± 0.038	3.696PheGly: 3.696 ± 0.051	0.74PheHis: 0.74 ± 0.023	1.419PheIle: 1.419 ± 0.036	0.836PheLys: 0.836 ± 0.031	3.552PheLeu: 3.552 ± 0.059	0.661PheMet: 0.661 ± 0.024	0.879PheAsn: 0.879 ± 0.029	1.592PhePro: 1.592 ± 0.04	0.876PheGln: 0.876 ± 0.027	2.468PheArg: 2.468 ± 0.046	2.39PheSer: 2.39 ± 0.047	1.817PheThr: 1.817 ± 0.038	3.193PheVal: 3.193 ± 0.049	0.503PheTrp: 0.503 ± 0.022	0.915PheTyr: 0.915 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	12.324GlyAla: 12.324 ± 0.131	0.787GlyCys: 0.787 ± 0.028	4.467GlyAsp: 4.467 ± 0.072	5.187GlyGlu: 5.187 ± 0.079	3.674GlyPhe: 3.674 ± 0.059	7.74GlyGly: 7.74 ± 0.13	1.664GlyHis: 1.664 ± 0.036	2.473GlyIle: 2.473 ± 0.042	2.791GlyLys: 2.791 ± 0.05	8.647GlyLeu: 8.647 ± 0.09	1.62GlyMet: 1.62 ± 0.039	1.709GlyAsn: 1.709 ± 0.054	3.4GlyPro: 3.4 ± 0.051	2.118GlyGln: 2.118 ± 0.043	6.852GlyArg: 6.852 ± 0.091	4.401GlySer: 4.401 ± 0.093	3.066GlyThr: 3.066 ± 0.064	7.491GlyVal: 7.491 ± 0.082	1.177GlyTrp: 1.177 ± 0.036	2.045GlyTyr: 2.045 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.384HisAla: 2.384 ± 0.053	0.246HisCys: 0.246 ± 0.014	1.148HisAsp: 1.148 ± 0.033	1.139HisGlu: 1.139 ± 0.029	0.849HisPhe: 0.849 ± 0.028	1.919HisGly: 1.919 ± 0.039	0.528HisHis: 0.528 ± 0.028	0.94HisIle: 0.94 ± 0.028	0.453HisLys: 0.453 ± 0.021	1.833HisLeu: 1.833 ± 0.042	0.408HisMet: 0.408 ± 0.02	0.392HisAsn: 0.392 ± 0.018	1.235HisPro: 1.235 ± 0.035	0.398HisGln: 0.398 ± 0.021	1.439HisArg: 1.439 ± 0.031	0.96HisSer: 0.96 ± 0.031	0.67HisThr: 0.67 ± 0.027	1.58HisVal: 1.58 ± 0.038	0.301HisTrp: 0.301 ± 0.014	0.531HisTyr: 0.531 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.453IleAla: 7.453 ± 0.083	0.449IleCys: 0.449 ± 0.019	3.803IleAsp: 3.803 ± 0.054	3.764IleGlu: 3.764 ± 0.058	1.55IlePhe: 1.55 ± 0.031	4.834IleGly: 4.834 ± 0.068	0.795IleHis: 0.795 ± 0.027	1.838IleIle: 1.838 ± 0.036	1.103IleLys: 1.103 ± 0.031	3.906IleLeu: 3.906 ± 0.064	0.738IleMet: 0.738 ± 0.024	0.976IleAsn: 0.976 ± 0.031	1.868IlePro: 1.868 ± 0.04	0.869IleGln: 0.869 ± 0.028	2.886IleArg: 2.886 ± 0.044	2.422IleSer: 2.422 ± 0.047	1.872IleThr: 1.872 ± 0.048	4.388IleVal: 4.388 ± 0.059	0.437IleTrp: 0.437 ± 0.018	1.046IleTyr: 1.046 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.821LysAla: 3.821 ± 0.065	0.203LysCys: 0.203 ± 0.013	1.452LysAsp: 1.452 ± 0.033	1.545LysGlu: 1.545 ± 0.044	0.902LysPhe: 0.902 ± 0.027	2.415LysGly: 2.415 ± 0.049	0.512LysHis: 0.512 ± 0.021	1.787LysIle: 1.787 ± 0.041	1.201LysLys: 1.201 ± 0.042	3.028LysLeu: 3.028 ± 0.058	0.662LysMet: 0.662 ± 0.023	0.783LysAsn: 0.783 ± 0.029	1.764LysPro: 1.764 ± 0.049	0.812LysGln: 0.812 ± 0.027	2.371LysArg: 2.371 ± 0.052	1.785LysSer: 1.785 ± 0.038	1.881LysThr: 1.881 ± 0.045	1.855LysVal: 1.855 ± 0.045	0.33LysTrp: 0.33 ± 0.017	0.562LysTyr: 0.562 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	15.977LeuAla: 15.977 ± 0.159	0.996LeuCys: 0.996 ± 0.027	6.535LeuAsp: 6.535 ± 0.073	5.837LeuGlu: 5.837 ± 0.08	3.663LeuPhe: 3.663 ± 0.059	8.234LeuGly: 8.234 ± 0.088	1.918LeuHis: 1.918 ± 0.044	4.33LeuIle: 4.33 ± 0.064	2.728LeuLys: 2.728 ± 0.055	10.055LeuLeu: 10.055 ± 0.114	1.848LeuMet: 1.848 ± 0.041	1.896LeuAsn: 1.896 ± 0.04	5.411LeuPro: 5.411 ± 0.069	2.616LeuGln: 2.616 ± 0.044	8.503LeuArg: 8.503 ± 0.096	6.26LeuSer: 6.26 ± 0.078	5.565LeuThr: 5.565 ± 0.092	7.634LeuVal: 7.634 ± 0.096	1.153LeuTrp: 1.153 ± 0.035	2.076LeuTyr: 2.076 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.504MetAla: 2.504 ± 0.046	0.127MetCys: 0.127 ± 0.011	0.868MetAsp: 0.868 ± 0.027	0.953MetGlu: 0.953 ± 0.031	0.612MetPhe: 0.612 ± 0.02	1.286MetGly: 1.286 ± 0.035	0.311MetHis: 0.311 ± 0.015	1.111MetIle: 1.111 ± 0.03	0.725MetLys: 0.725 ± 0.026	2.021MetLeu: 2.021 ± 0.04	0.465MetMet: 0.465 ± 0.02	0.607MetAsn: 0.607 ± 0.02	1.001MetPro: 1.001 ± 0.028	0.621MetGln: 0.621 ± 0.02	1.961MetArg: 1.961 ± 0.043	1.499MetSer: 1.499 ± 0.036	1.565MetThr: 1.565 ± 0.036	1.074MetVal: 1.074 ± 0.029	0.175MetTrp: 0.175 ± 0.011	0.189MetTyr: 0.189 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.887AsnAla: 2.887 ± 0.056	0.217AsnCys: 0.217 ± 0.012	1.2AsnAsp: 1.2 ± 0.029	1.18AsnGlu: 1.18 ± 0.031	0.963AsnPhe: 0.963 ± 0.034	2.162AsnGly: 2.162 ± 0.061	0.435AsnHis: 0.435 ± 0.019	1.198AsnIle: 1.198 ± 0.032	0.565AsnLys: 0.565 ± 0.019	2.271AsnLeu: 2.271 ± 0.048	0.444AsnMet: 0.444 ± 0.017	0.607AsnAsn: 0.607 ± 0.03	1.343AsnPro: 1.343 ± 0.037	0.582AsnGln: 0.582 ± 0.022	1.549AsnArg: 1.549 ± 0.036	1.2AsnSer: 1.2 ± 0.038	0.825AsnThr: 0.825 ± 0.031	1.89AsnVal: 1.89 ± 0.044	0.356AsnTrp: 0.356 ± 0.017	0.636AsnTyr: 0.636 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	6.955ProAla: 6.955 ± 0.093	0.373ProCys: 0.373 ± 0.018	3.174ProAsp: 3.174 ± 0.05	3.379ProGlu: 3.379 ± 0.066	2.071ProPhe: 2.071 ± 0.042	4.329ProGly: 4.329 ± 0.061	1.099ProHis: 1.099 ± 0.032	2.494ProIle: 2.494 ± 0.042	1.612ProLys: 1.612 ± 0.04	5.152ProLeu: 5.152 ± 0.061	0.992ProMet: 0.992 ± 0.031	1.206ProAsn: 1.206 ± 0.031	3.352ProPro: 3.352 ± 0.068	1.851ProGln: 1.851 ± 0.037	3.753ProArg: 3.753 ± 0.059	3.216ProSer: 3.216 ± 0.058	2.541ProThr: 2.541 ± 0.051	3.423ProVal: 3.423 ± 0.051	0.623ProTrp: 0.623 ± 0.024	1.109ProTyr: 1.109 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	3.51GlnAla: 3.51 ± 0.058	0.179GlnCys: 0.179 ± 0.012	1.286GlnAsp: 1.286 ± 0.034	1.432GlnGlu: 1.432 ± 0.034	0.99GlnPhe: 0.99 ± 0.037	1.965GlnGly: 1.965 ± 0.038	0.483GlnHis: 0.483 ± 0.021	1.772GlnIle: 1.772 ± 0.054	1.036GlnLys: 1.036 ± 0.032	2.587GlnLeu: 2.587 ± 0.056	0.627GlnMet: 0.627 ± 0.023	0.751GlnAsn: 0.751 ± 0.027	1.409GlnPro: 1.409 ± 0.035	0.971GlnGln: 0.971 ± 0.03	2.328GlnArg: 2.328 ± 0.044	1.759GlnSer: 1.759 ± 0.04	1.612GlnThr: 1.612 ± 0.039	1.555GlnVal: 1.555 ± 0.043	0.421GlnTrp: 0.421 ± 0.017	0.526GlnTyr: 0.526 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	10.797ArgAla: 10.797 ± 0.135	0.661ArgCys: 0.661 ± 0.024	4.417ArgAsp: 4.417 ± 0.072	4.904ArgGlu: 4.904 ± 0.072	3.432ArgPhe: 3.432 ± 0.061	5.622ArgGly: 5.622 ± 0.075	1.703ArgHis: 1.703 ± 0.038	4.098ArgIle: 4.098 ± 0.056	2.352ArgLys: 2.352 ± 0.04	9.751ArgLeu: 9.751 ± 0.112	1.747ArgMet: 1.747 ± 0.04	1.808ArgAsn: 1.808 ± 0.036	4.026ArgPro: 4.026 ± 0.069	2.184ArgGln: 2.184 ± 0.044	7.894ArgArg: 7.894 ± 0.122	4.297ArgSer: 4.297 ± 0.068	2.984ArgThr: 2.984 ± 0.047	5.247ArgVal: 5.247 ± 0.07	1.064ArgTrp: 1.064 ± 0.031	1.764ArgTyr: 1.764 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.272SerAla: 7.272 ± 0.1	0.544SerCys: 0.544 ± 0.027	2.853SerAsp: 2.853 ± 0.044	2.895SerGlu: 2.895 ± 0.054	2.587SerPhe: 2.587 ± 0.041	5.474SerGly: 5.474 ± 0.098	1.006SerHis: 1.006 ± 0.031	2.692SerIle: 2.692 ± 0.054	1.43SerLys: 1.43 ± 0.039	5.867SerLeu: 5.867 ± 0.083	1.057SerMet: 1.057 ± 0.03	1.374SerAsn: 1.374 ± 0.048	3.015SerPro: 3.015 ± 0.05	1.395SerGln: 1.395 ± 0.034	4.109SerArg: 4.109 ± 0.063	3.413SerSer: 3.413 ± 0.075	2.753SerThr: 2.753 ± 0.071	3.91SerVal: 3.91 ± 0.053	0.807SerTrp: 0.807 ± 0.029	1.415SerTyr: 1.415 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	5.9ThrAla: 5.9 ± 0.072	0.371ThrCys: 0.371 ± 0.019	2.246ThrAsp: 2.246 ± 0.044	2.187ThrGlu: 2.187 ± 0.042	1.719ThrPhe: 1.719 ± 0.034	4.263ThrGly: 4.263 ± 0.076	0.962ThrHis: 0.962 ± 0.03	2.824ThrIle: 2.824 ± 0.063	1.47ThrLys: 1.47 ± 0.039	5.785ThrLeu: 5.785 ± 0.08	0.899ThrMet: 0.899 ± 0.027	1.213ThrAsn: 1.213 ± 0.038	3.454ThrPro: 3.454 ± 0.057	1.486ThrGln: 1.486 ± 0.078	3.729ThrArg: 3.729 ± 0.052	2.775ThrSer: 2.775 ± 0.067	2.875ThrThr: 2.875 ± 0.111	3.24ThrVal: 3.24 ± 0.06	0.43ThrTrp: 0.43 ± 0.018	1.054ThrTyr: 1.054 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	10.492ValAla: 10.492 ± 0.102	0.637ValCys: 0.637 ± 0.022	4.579ValAsp: 4.579 ± 0.053	5.023ValGlu: 5.023 ± 0.069	2.63ValPhe: 2.63 ± 0.048	5.549ValGly: 5.549 ± 0.078	1.214ValHis: 1.214 ± 0.028	3.32ValIle: 3.32 ± 0.048	2.202ValLys: 2.202 ± 0.053	6.457ValLeu: 6.457 ± 0.072	1.415ValMet: 1.415 ± 0.035	1.784ValAsn: 1.784 ± 0.043	2.819ValPro: 2.819 ± 0.05	1.648ValGln: 1.648 ± 0.036	4.558ValArg: 4.558 ± 0.056	4.266ValSer: 4.266 ± 0.067	4.259ValThr: 4.259 ± 0.066	5.956ValVal: 5.956 ± 0.08	0.764ValTrp: 0.764 ± 0.026	1.498ValTyr: 1.498 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.139TrpAla: 1.139 ± 0.028	0.122TrpCys: 0.122 ± 0.01	0.512TrpAsp: 0.512 ± 0.024	0.476TrpGlu: 0.476 ± 0.017	0.469TrpPhe: 0.469 ± 0.02	0.889TrpGly: 0.889 ± 0.029	0.233TrpHis: 0.233 ± 0.013	0.577TrpIle: 0.577 ± 0.023	0.378TrpLys: 0.378 ± 0.017	1.489TrpLeu: 1.489 ± 0.044	0.284TrpMet: 0.284 ± 0.014	0.375TrpAsn: 0.375 ± 0.017	0.674TrpPro: 0.674 ± 0.027	0.354TrpGln: 0.354 ± 0.016	1.61TrpArg: 1.61 ± 0.042	0.931TrpSer: 0.931 ± 0.035	0.786TrpThr: 0.786 ± 0.026	0.576TrpVal: 0.576 ± 0.024	0.201TrpTrp: 0.201 ± 0.02	0.197TrpTyr: 0.197 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.606TyrAla: 2.606 ± 0.043	0.25TyrCys: 0.25 ± 0.013	1.483TyrAsp: 1.483 ± 0.045	1.213TyrGlu: 1.213 ± 0.029	0.922TyrPhe: 0.922 ± 0.031	2.006TyrGly: 2.006 ± 0.046	0.471TyrHis: 0.471 ± 0.02	0.719TyrIle: 0.719 ± 0.025	0.557TyrLys: 0.557 ± 0.021	2.12TyrLeu: 2.12 ± 0.047	0.313TyrMet: 0.313 ± 0.015	0.539TyrAsn: 0.539 ± 0.023	1.051TyrPro: 1.051 ± 0.027	0.61TyrGln: 0.61 ± 0.021	1.822TyrArg: 1.822 ± 0.039	1.21TyrSer: 1.21 ± 0.032	0.922TyrThr: 0.922 ± 0.029	1.59TyrVal: 1.59 ± 0.039	0.338TyrTrp: 0.338 ± 0.015	0.56TyrTyr: 0.56 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4187 proteins (1315237 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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