Bacteroides caccae
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4410 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A174PHH8|A0A174PHH8_9BACE OmpA family protein OS=Bacteroides caccae OX=47678 GN=DW080_00610 PE=4 SV=1
MM1 pKa = 7.49 LRR3 pKa = 11.84 FKK5 pKa = 10.74 FGAWAVVLMLAALSFGACSDD25 pKa = 4.11 EE26 pKa = 5.83 DD27 pKa = 4.73 DD28 pKa = 5.41 DD29 pKa = 5.4 PAFNPPSNITEE40 pKa = 4.19 ALKK43 pKa = 10.48 QLYY46 pKa = 8.51 PAAQNIEE53 pKa = 3.87 WEE55 pKa = 4.46 MKK57 pKa = 9.9 DD58 pKa = 3.3 VYY60 pKa = 11.02 YY61 pKa = 11.1 VADD64 pKa = 3.77 CWVADD69 pKa = 4.41 DD70 pKa = 4.88 EE71 pKa = 5.31 LEE73 pKa = 4.1 VWFDD77 pKa = 4.44 GNANWLMTEE86 pKa = 4.35 NEE88 pKa = 4.41 LEE90 pKa = 4.67 SIDD93 pKa = 3.97 SLVPAVYY100 pKa = 8.57 TAFNNTKK107 pKa = 8.26 YY108 pKa = 10.69 SSWVVTDD115 pKa = 3.5 VYY117 pKa = 11.58 VLVYY121 pKa = 8.64 PQNPTEE127 pKa = 4.34 SVIQVKK133 pKa = 10.04 QGSRR137 pKa = 11.84 RR138 pKa = 11.84 YY139 pKa = 10.32 SLVFSQDD146 pKa = 2.59 GGLLHH151 pKa = 7.06 EE152 pKa = 5.21 KK153 pKa = 10.31 DD154 pKa = 3.85 ISNGDD159 pKa = 3.5 DD160 pKa = 4.1 TIWPPAEE167 pKa = 3.8
Molecular weight: 18.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.656
ProMoST 3.986
Dawson 3.846
Bjellqvist 4.05
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.901
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A174K0M3|A0A174K0M3_9BACE Anti-sigma factor antagonist OS=Bacteroides caccae OX=47678 GN=btrW_1 PE=3 SV=1
MM1 pKa = 7.17 EE2 pKa = 5.67 RR3 pKa = 11.84 KK4 pKa = 9.32 RR5 pKa = 11.84 NYY7 pKa = 9.3 IEE9 pKa = 5.44 KK10 pKa = 10.04 VRR12 pKa = 11.84 DD13 pKa = 3.22 WADD16 pKa = 3.0 DD17 pKa = 3.52 RR18 pKa = 11.84 LRR20 pKa = 11.84 RR21 pKa = 11.84 MCGRR25 pKa = 11.84 ITPGKK30 pKa = 8.59 RR31 pKa = 11.84 LAVILVMFFFFGGLSIYY48 pKa = 9.37 ITVSSIYY55 pKa = 10.61 NIGKK59 pKa = 9.55 RR60 pKa = 11.84 DD61 pKa = 3.69 GQRR64 pKa = 11.84 LQIEE68 pKa = 5.41 HH69 pKa = 6.74 IRR71 pKa = 11.84 QLPLHH76 pKa = 6.64 GNDD79 pKa = 2.91 TTKK82 pKa = 10.96 NINPLNRR89 pKa = 11.84 PQYY92 pKa = 10.61 GRR94 pKa = 11.84 STEE97 pKa = 4.01 KK98 pKa = 10.93
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.765
IPC_protein 10.613
Toseland 10.672
ProMoST 10.452
Dawson 10.789
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 10.979
Grimsley 10.847
Solomon 10.891
Lehninger 10.862
Nozaki 10.643
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.716
Patrickios 10.716
IPC_peptide 10.906
IPC2_peptide 9.472
IPC2.peptide.svr19 8.538
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4410
0
4410
1589254
29
2262
360.4
40.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.817 ± 0.035
1.24 ± 0.014
5.465 ± 0.021
6.671 ± 0.039
4.594 ± 0.024
6.715 ± 0.032
1.819 ± 0.014
6.983 ± 0.035
6.818 ± 0.03
9.089 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.015
5.237 ± 0.033
3.718 ± 0.019
3.47 ± 0.02
4.617 ± 0.026
6.174 ± 0.03
5.662 ± 0.026
6.363 ± 0.029
1.29 ± 0.015
4.54 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here