Saimiri sciureus polyomavirus 1
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7QJK3|K7QJK3_9POLY Agnoprotein OS=Saimiri sciureus polyomavirus 1 OX=1236410 PE=4 SV=1
MM1 pKa = 7.31 GALLAVLAEE10 pKa = 4.29 VVEE13 pKa = 4.39 LASVTGLSVEE23 pKa = 4.35 SFLSGEE29 pKa = 4.19 AFATAEE35 pKa = 4.19 LLEE38 pKa = 3.97 AHH40 pKa = 7.15 IANLVTVGGLTEE52 pKa = 4.49 AEE54 pKa = 3.83 ALAATEE60 pKa = 4.38 VSAEE64 pKa = 4.01 AYY66 pKa = 9.14 AALTSLSSTFPQAFTAVAATEE87 pKa = 4.01 LATTGTLTVGATVAAALYY105 pKa = 8.79 PYY107 pKa = 9.81 YY108 pKa = 10.37 YY109 pKa = 10.45 DD110 pKa = 3.48 YY111 pKa = 10.56 STPVANLNRR120 pKa = 11.84 GLNPEE125 pKa = 4.22 MALQLWFPEE134 pKa = 3.59 IDD136 pKa = 3.62 YY137 pKa = 9.26 EE138 pKa = 5.16 FPGLMPFVRR147 pKa = 11.84 FINYY151 pKa = 9.15 IDD153 pKa = 3.59 PTQWATNLFEE163 pKa = 4.62 TIGRR167 pKa = 11.84 YY168 pKa = 7.52 FWEE171 pKa = 4.12 SAQRR175 pKa = 11.84 YY176 pKa = 5.48 GQNLIAHH183 pKa = 6.71 EE184 pKa = 4.3 VRR186 pKa = 11.84 SASRR190 pKa = 11.84 EE191 pKa = 3.93 LATRR195 pKa = 11.84 TAQGFSEE202 pKa = 4.95 AIARR206 pKa = 11.84 YY207 pKa = 9.4 FEE209 pKa = 3.86 NARR212 pKa = 11.84 WAVSMLPRR220 pKa = 11.84 SLYY223 pKa = 10.8 SGLQSYY229 pKa = 9.15 YY230 pKa = 10.58 EE231 pKa = 4.12 QLPSLNPMQVRR242 pKa = 11.84 DD243 pKa = 3.22 LHH245 pKa = 6.68 RR246 pKa = 11.84 RR247 pKa = 11.84 LGQPIPNRR255 pKa = 11.84 IALEE259 pKa = 3.91 EE260 pKa = 3.87 QAIKK264 pKa = 10.32 SAEE267 pKa = 4.05 YY268 pKa = 8.38 VQKK271 pKa = 10.95 VEE273 pKa = 4.42 PPGGANQRR281 pKa = 11.84 IAPDD285 pKa = 3.16 WLLPLILGLYY295 pKa = 10.37 GDD297 pKa = 5.2 ISPSWEE303 pKa = 3.98 STLEE307 pKa = 4.53 DD308 pKa = 3.47 IEE310 pKa = 4.55 EE311 pKa = 4.46 EE312 pKa = 3.8 EE313 pKa = 5.56 DD314 pKa = 3.62 APQKK318 pKa = 10.61 KK319 pKa = 9.26 KK320 pKa = 10.7 RR321 pKa = 11.84 KK322 pKa = 8.58 RR323 pKa = 11.84 SKK325 pKa = 10.89 KK326 pKa = 7.74 NTSRR330 pKa = 11.84 SAA332 pKa = 3.04
Molecular weight: 36.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.808
IPC2_protein 4.876
IPC_protein 4.774
Toseland 4.647
ProMoST 4.838
Dawson 4.685
Bjellqvist 4.838
Wikipedia 4.507
Rodwell 4.622
Grimsley 4.571
Solomon 4.685
Lehninger 4.635
Nozaki 4.8
DTASelect 4.863
Thurlkill 4.635
EMBOSS 4.533
Sillero 4.876
Patrickios 3.783
IPC_peptide 4.698
IPC2_peptide 4.876
IPC2.peptide.svr19 4.84
Protein with the highest isoelectric point:
>tr|K7QK08|K7QK08_9POLY Capsid protein VP1 OS=Saimiri sciureus polyomavirus 1 OX=1236410 PE=3 SV=1
MM1 pKa = 6.96 VAITIKK7 pKa = 10.53 LWLHH11 pKa = 4.92 ARR13 pKa = 11.84 KK14 pKa = 9.5 LKK16 pKa = 10.22 PQMARR21 pKa = 11.84 KK22 pKa = 9.75 LMYY25 pKa = 10.17 RR26 pKa = 11.84 KK27 pKa = 7.66 TPPLVGLDD35 pKa = 3.51 TAQLRR40 pKa = 11.84 VEE42 pKa = 4.78 IIWLTSQMDD51 pKa = 4.24 LFVLTDD57 pKa = 3.27 KK58 pKa = 11.7 GEE60 pKa = 4.23 TRR62 pKa = 11.84 ITAASALRR70 pKa = 11.84 AVTFAMTSVSLKK82 pKa = 10.43 NSCFF86 pKa = 3.51
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 9.604
IPC_protein 9.838
Toseland 10.774
ProMoST 10.277
Dawson 10.833
Bjellqvist 10.438
Wikipedia 10.965
Rodwell 11.301
Grimsley 10.847
Solomon 10.906
Lehninger 10.891
Nozaki 10.745
DTASelect 10.438
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.76
Patrickios 11.082
IPC_peptide 10.921
IPC2_peptide 9.092
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1595
86
657
319.0
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.332 ± 2.102
2.508 ± 0.911
4.577 ± 0.65
7.273 ± 0.868
4.953 ± 0.788
5.141 ± 0.552
1.693 ± 0.306
4.577 ± 0.33
7.712 ± 1.549
11.473 ± 0.915
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.696 ± 0.521
4.828 ± 0.783
5.078 ± 0.847
4.013 ± 0.079
3.386 ± 0.743
6.019 ± 0.443
6.583 ± 0.436
6.332 ± 0.745
1.379 ± 0.319
3.448 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here